摘要:

It has been found possible to test ferritin concentrations in the reticulum and hemopoietic cells from human bone marrow by an immunoradiometric assay. Ferritin concentration in healthy test persons amounts to 0.92 ± 0.38 ng/μg protein in the reticulum and 0.084 ± 0.031 ng/μg protein in hemopoietic cells. In healthy test persons about 90% of the ferritin is localized in the reticulum and about 10% in the hemopoietic cells. The ferritin concentration in the reticulum is decreased in patients having iron deficiency anemia. In these patients only 50–70% of the bone marrow ferritin is localized in the reticulum. Bone marrow ferritin was characterized by a combination of anion-exchange chromatography using Sephadex A-50 and an immunoradiometric assay. Marrow ferritin from healthy persons is eluted at chloride concentrations between 200 and 300 mM. The anion-exchange chromatographic properties of ferritin in the reticulum and hemopoietic cells are iden

ISSN:0001-5792    

DOI:10.1159/000207030

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

New pyroglobulin variants were found in a patient with a typical plasma cell leukemia. One was found in the urine and proved to be a variant of Bence-Jones’ protein with a molecular weight of approximately 68,000. The other was found in the serum and appears to be the myeloma protein of IgD. Furthermore, an ultrastructural study disclosed myeloma cells in both peripheral blood and bone marrow to have cytoplasmic fibrils which might be unique for plasma cell leukemi

ISSN:0001-5792    

DOI:10.1159/000207031

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Inferior venacavography was used for revealing enlarged retro- or paracaval lymph nodes, some of which are not visualized by lymphography as they lay above L 2. As criteria for involvement of lymph nodes the dislocation or narrowing of the vena cava and the reflux of contrast medium in the lumbar veins were studied. Venacavography was carried out in 48 consecutive patients with lymphoma: 23 Hodgkin’s disease and 25 non-Hodgkin’s lymphoma. Signs of enlargement of paracaval lymph nodes were seen in 52% of patients with Hodgkin’s disease and in 80% of the cases of non-Hodgkin’s lymphoma. A fairly good agreement was found between lymphograms and cavograms. Cavography proved to be an easy, inexpensive and minimally invasive technique that can be repeated several times without inconveniencing the patients too much, thus enabling restaging of patients after therapy; moreover, it produced fairly good urograms as the contrast medium is promptly eliminated through the kidney, thus eliminating the need for intravenous pyel

ISSN:0001-5792    

DOI:10.1159/000207032

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A 17-year-old male patient with aplastic anemia underwent bone marrow transplantation and succumbed 4 days after marrow infusion from sudden myocardial failure. Fever of unknown origin (FUO) had accompanied the patients course from admission until death. The cause of death was fungus myocarditis, which had escaped detection in vivo, in spite of a daily culture program for bacteria and fungi, and a close monitoring of the patients circulation and ventricular performance. Commonly applied diagnostic criteria for systemic mycosis, such as topical colonization, malfunction of invaded organs and positive fungus cultures failed to provide a timely diagnosis. With regard to the problems in diagnosing systemic mycosis, the potential stem cell toxicity of antifungal drugs and the need for immunosuppressive therapy prior to marrow infusion, we strongly recommend not to start the transplantation procedure unless FUO has been treated successfully.

ISSN:0001-5792    

DOI:10.1159/000207033

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The adaptation of cell physiological parameters of erythrocytes of the newborn to erythrocytes in later life was studied by cell separation in an isopycnic dextran density gradient. The cell characteristics during the first trimester of life were followed by repeated determinations. The mean cellular haemoglobin concentration of the lightest cells is remarkably less than that of the cells of median density. This is probably due to the ongoing synthesis of haemoglobin in reticulocytes, which are highly concentrated in the top fractions. It was shown that the young cells released from the bone marrow become progressively smaller during this period with a decrease of mean cellular volume (MCV) from 104 μm3 at birth to 85 μm3 at the end of the first trimester. It has been established that this process commences during the first week of life. No difference concerning the alterations of cell parameters, especially MCV, in premature and mature infants was found. It is therefore probable that these alterations are due to changes in the external milieu following birt

ISSN:0001-5792    

DOI:10.1159/000207034

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Using an improved method of determination, urinary trypsin inhibitor (UTI) activities were assayed in relation to urokinase (UK) activities in a large group of patients with various renal diseases. In normal subjects (n = 50), the mean 24-hour values of the UTI and UK activities were 4.29 ± 1.44 U/ml and 9.80 ± 3.81 IU/ml, respectively. Data for renal diseases such as renal stone, hydronephrosis, renal cancer, and chronic glomerulo-nephritis (UTI, 5.51 ± 2.29 U/ml (p < 0.005) and UK, 6.88 ± 2.64 IU/ml (p < 0.001); n = 40), and particularly uremia (UTI, 9.90 ± 5.68 U/ml (p < 0.001) and UK, 3.85 ± 2.36 IU/ml (p < 0.001); n = 30), showed that the UTI level was increased whereas the UK level was decreased. The UTI/UK ratio more clearly demonstrated the difference between these dis

ISSN:0001-5792    

DOI:10.1159/000207035

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

α-Tocopherol (vitamin E) inhibited the activity of urokinase on fibrin plates and on the amidolytic substrate S-2444 in a concentration- and time-dependent manner. Inhibition was not removed by dialysis. In the presence of dilutions of plasma, protein-containing fractions of gel-filtered plasma, or bovine serum albumin, the inhibition of urokinase by α-tocopherol was abolished. Urokinase activity was not inhibited by phytol, menadione or hydroquinon

ISSN:0001-5792    

DOI:10.1159/000207036

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A patient with thrombotic thrombocytopenic purpura was treated with 8 U of fresh frozen plasma, resulting in a rapid improvement in her condition and subsequently followed by a prolonged clinical remission. The transmission and scanning electron microscopic examinations of the platelets performed before treatment demonstrated marked anomalies of the platelet morphology, namely, an increased number of abnormally elongated pseudopods with terminal knobs, a decreased number of platelet granules and excess of glycogen and marked anisocytosis, These anomalies remained unchanged when reexamined 2 months after the achievement of the clinical remission. The question whether these platelet abnormalities are an integral part of thrombotic thrombocytopenic purpura and play a role in its pathogenesis, is raised.

ISSN:0001-5792    

DOI:10.1159/000207037

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Patients with liver disease have abnormal platelet function. We have investigated the retention of platelets to glass beads in fulminant hepatic failure, cirrhosis and normal controls. Glass bead retention was significantly increased in fulminant hepatic failure (91.5 ± 4.8%, n = 10) compared with controls (80.2 ± 8.5%, n = 10) whereas it was significantly reduced in cirrhosis (46.2 ± 26.8%, n = 10). The increased platelet adhesiveness might predispose to the formation of platelet aggregates in the blood observed during charcoal haemoperfusion in patients with fulminant hepatic failu

ISSN:0001-5792    

DOI:10.1159/000207038

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A circulating anticoagulant against factor XII was detected in a patient with smoldering leukemia. Despite severe associated thrombocytopenia the patient suffered two thromboembolic episodes, besides mucosal bleeding. The circulating anticoagulant was demonstrated not only in the plasma but also in the serum. Its activity was not affected by heating at 56° C for 30 min and it was not adsorbed by S04Ba or A1(OH)3. The circulating anticoagulant was not dializable and demonstrated to be an IgG. This is apparently the first reported association of smoldering leukemia and a circulating anticoagulant against factor XII

ISSN:0001-5792    

DOI:10.1159/000207039

出版商:S. Karger AG    

年代:1982

数据来源: Karger