摘要:

A solid-phase immunoradiometric assay for serum ferritin has been developed and applied to 30 patients with chronic myeloid leukemia (CML), including 13 patients with a typical chronic phase, 12 with bone marrow fibrosis without evidence of an acute phase and 5 with an acute phase. Patients were divided in to three subgroups according to the erythrokinetic data as studied by the 59iron kinetic test. Serum ferritin was clearly correlated with the quality of erythropoiesis but not with blast cell mass nor white cell count. Given the prognostic value of dyserythropoiesis in the course of CML, serum ferritin determination may be a helpful and simple parameter in the follow-up of CML patients.

ISSN:0001-5792    

DOI:10.1159/000207046

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

G-banded cytogenetic studies of 3 male patients in the terminal phase of chronic myeloid leukemia showed the following abnormalities: in the first case, the presence of a medullar cell line with 51 chromosomes and 3 Ph1; in the second case, a clone with 65 chromosomes and 4 Ph1, and in the third patient a clone with 53 chromosomes and 3 Ph1. In all 3 cases, G-banding revealed the Ph1 translocation to be of the usual type: t(9;22) (q34;q11) and there was discordance between the number of Ph1 and 9q+. There was no obvious correlation between the presence of multiple Ph1 and the clinical or cytological features. These 3 cases were detected as part of a recent G-banded cytogenetic survey of 9 individuals in the blastic phase studied by the authors. The frequency of multiple Ph1 observed in this sample of blastic-phase leukemia is unusually elevated, raising the question of the origin of such a high incidence.

ISSN:0001-5792    

DOI:10.1159/000207047

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Bone marrow and blood smears from patients with acute lymphoblastic and myeloblastic leukaemia prior to therapy were stained for tetrahydrofolate dehydrogenase. Compared with other cytochemical tests this reaction seems to discriminate clearly between cells with myeloid and lymphoid features. Lymphoblasts were intensely positive, the reaction being located asymmetrically at one pole of the cell, while myeloid malignant cells were slightly positive with evenly diffused granules in the cytoplasm. Thus, tetrahydrofolate dehydrogenase reaction should be an effective marker of blast cells indistinguishable by other characteristics, as found in unclassifiable leukaemia.

ISSN:0001-5792    

DOI:10.1159/000207048

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

12 out of 160 cases of acute lymphoblastic leukemias (ALL) when studied for their cell surface markers demonstrated the presence of dual surface markers. Cytochemical analysis showed a strong acid phosphatase staining in these dual marker ALL. Clinically, all these cases resemble T cell type ALL with poor prognosis.

ISSN:0001-5792    

DOI:10.1159/000207049

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

150 patients with acute myeloblastic leukemia were admitted from 1971 to 1980; 59 were more than 55 years old. For remission induction, three polychemotherapeutic schedules including different doses of anthracyclines were used. Complete remission was obtained in about 50% of patients less than 55 years, whereas in the elderly people complete remission rate ranged from 12 to 32%. The median duration of complete remission was, in this latter group of patients, 3 months and did not appear to be related to the aggressive regimen employed during the induction phase.

ISSN:0001-5792    

DOI:10.1159/000207050

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

In order to evaluate the validity of 3H-diisopropyl fluorophosphate (DFP) as a label for neutrophil kinetic studies, the blood and skin chamber kinetics of autologous neutrophils doubly labeled with 3H-DFP and 32P-DFP were determined in 6 normal subjects. Blood disappearance time and skin chamber accumulation of label was slightly but significantly decreased using 3H values, indicating mild elution of the isotope from the cells. The degree of elution was small in comparison to the previously observed rate of elution of 32P-DFP and suggests that 3H-DFP is an acceptable alternative label.

ISSN:0001-5792    

DOI:10.1159/000207051

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Neutrophil myeloperoxidase (MPO) activity was analyzed by a semi-quantitative cytochemical method in 268 subjects divided into several groups. 17 subjects with significantly reduced MPO activity were found: 11 of 23 in the preleukemia group, 2/14 AMLs, 1/20 myeloproliferative syndrome, 1/7 carcinoma with bone marrow metastases, 1/33 diabetes mellitus and 1/50 normals. Only in the preleukemia group, was MPO significantly reduced in comparison to the normal group (p < 0.005). The high frequency of acquired MPO deficiency in preleukemia represents a useful criterium for this diagnosis. Furthermore, in these patients, as well as in the other subjects studied, no apparent correlation between MPO level and infection could be demonstrated.

ISSN:0001-5792    

DOI:10.1159/000207052

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Osteopetrosis is a congenital disease in which the bone marrow is replaced by osteoid, resulting in secondary extrameduUary hematopoiesis. In 3 patients with osteopetrosis, circulating myeloid and erythroid progenitors were assayed. Erythroid burst-forming units were increased above normal by a factor of five and the myeloid colony-forming units were increased by a factor of ten. Myeloid cluster-forming units and erythroid colony-forming units, the more differentiated progenitor cells normally found only in the bone marrow, could also be detected in the peripheral blood of the 3 patients. The appearance of the differentiated progenitors in the peripheral blood suggests that extrameduUary hematopoietic sites, in contrast to the bone marrow, may release progenitor cells more readily into the circulation. If this is the case, culturing progenitor cells may provide a sensitive noninvasive laboratory test to determine the presence of extrameduUary hematopoiesis.

ISSN:0001-5792    

DOI:10.1159/000207053

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Five radioactive peaks can be identified when hemolysates prepared from 59Fe-labelled guinea pig reticulocytes are subjected to molecular sieve chromatography. The third peak, hemoglobin, is followed by another heme-containmg peak eluting with an apparent molecular weight of 17,000. We now demonstrate that this 17,000 MW peak consists of α-chains. The high specific activity (59Fe/heme) of these α-chains suggests that they may be the primary site of heme entry into the hemoglobin molecul

ISSN:0001-5792    

DOI:10.1159/000207054

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The serum levels, the biological activity and the phenotypes of α1-antitrypsin (α1AT) were determined in 158 /β-thalassaemic patients and in 504 healthy controls. The concentration and the activity of α1AT were found within normal limits. An increased frequency of the F gene, a decreased frequency of the M and a normal frequency of the Z gene were observed in thalassaemic patients. It can be suggested that the quantity and/or the activity of serum α1AT are not responsible for the respiratory infections encountered in many of the β-thalassaemic pat

ISSN:0001-5792    

DOI:10.1159/000207055

出版商:S. Karger AG    

年代:1982

数据来源: Karger