摘要:

Bone marrow samples of 28 individuals with clinically benign and of 41 patients with malignant monoclonal gammopathy were analyzed for the total number of lymphoplasmocellular elements containing cytoplasmic immunoglobulins and for the monoclonal fraction of these cells. Monoclonal immunoglobulin components were determined in sera. A discriminant analysis was performed on the data: the variables were transformed and in a stepwise procedure used for the construction of a discriminant function which by a dividing point allowed a good distinction between the two groups of patients. By use of this discriminant function, 91% of the patients in the sample were correctly classified.

ISSN:0001-5792    

DOI:10.1159/000207709

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Cytoplasmic immunoglobulins in human bone marrow plasma cells and lymphoid cells were characterized by direct immunofluorescence with fluorochrome-labelled reagents specific for immunoglobulin heavy and light chains. The percentage distribution of cells containing IgA, IgG or IgM and χ- or λ-immunoglobulins was determined in bone marrow samples from 168 immunologically normal individuals, in 11 patients with polyclonal increase of bone marrow plasma cells and in 80 patients with benign or malignant monoclonal gammopathies. A clear differentiation between monoclonal and polyclonal cell populations could be obtained in all case

ISSN:0001-5792    

DOI:10.1159/000207710

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

146 serial determinations of spontaneous and phytohemagglutinin-induced lymphocyte transformation as measured by tritiated thymidine incorporation, were performed in 24 healthy individuals. 9 persons were studied intensively during a mean period of 40 days and the other 15 underwent random investigations during a period up to 10 months. Transient episodes of significant decreased lymphocyte transformation were revealed in 14 persons (58.4%) with regard to the spontaneous blastogenesis and in 11 subjects (45.8%) with regard to PHA-induced reactivity. The occurrence of the decreased values in PHA responsiveness was significantly higher in the intensively studied group compared to the randomly investigated one (p = 0.001). The probability of detecting one low PHA-related response in a normal subject was 11%. In only half of the cultures with low spontaneous blastogenic response did a simultaneous decrease in PHA-induced lymphocyte transformation occur. In addition to the currently used stimulation index, a new index termed the Blastogenic Cumulative Index is proposed. It is defined as the sum of the logarithms of the dpm values of the spontaneous and the PHA induced lymphocyte transformation. The CI is suggested to describe lymphocyte blastogenic function more accurately, and due to its simplicity warrants further study. Other indices for lymphocyte blastogenesis determination are discussed.

ISSN:0001-5792    

DOI:10.1159/000207711

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

The ethnic distribution of 288 patients with chronic lymphocytic leukemia (CLL) from five medical centers in Israel during the decade 1960–1970 was investigated as well as other features of the disease. 80.5% of the patients were of East European descent, 14.5% originated from Asia and Africa and 5% were born in Israel. Based on the ethnic distribution of the elderly population of Israel during the above period, it is suggested that CLL occurs more frequently in European-born Jews than in Asian and African Jews. A low occurrence of Coombs positive hemolytic anemia was revealed, amounting to 7.2% of the patients. Of 68 patients who underwent serum immunoglobulin studies, up to 74% exhibited deficiency of at least one type of immunoglobulin. Low levels of IgA were encountered in 61.2%, IgM in 51.5% and IgG in 29.5% of the patients. 26 of 206 patients with CLL (12.5%), all of East European origin, had an additional primary malignant tumor, cancer of the skin and breast being the most frequent associated malignancies. In 6 patients of the 26, two additional neoplasms were diagnosed. Hypogammaglobulinemia was more frequent in patients with additional primary malignant tumors and in patients with advanced disease. The possible causes for the ethnic distribution of CLL in Israel as well as of other tumors are discusse

ISSN:0001-5792    

DOI:10.1159/000207712

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

The presence of in vitro colony-forming units (CFUc) was demonstrated in leukocyte suspensions collected by continuous flow leukaphereses performed serially in dogs. The CFUc yield of one leukapheresis was usually between 104 and 106. Higher yields were obtained in the 2nd to 5th leukaphereses than in the first one in the same dog. The number of CFUc collected during one leukapheresis was about four times the number of CFUc present in the circulating blood at the beginning of the leukapheresis. After a diminution during leukapheresis, the circulating CFUc pool was restored in 2 or 3 days with an increase in the ratio of CFUc to mononuclear cells. These results indicate that CFUc entered the circulating pool during and after leukapheresis and that the population size regulation of canine-circulating CFUc is different from that of the bulk of the mononuclear leukocytes.

ISSN:0001-5792    

DOI:10.1159/000207713

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

A precise, although technically complicated method is described for the estimation of iron absorption. A steel chamber is used to measure the total radioactivity of the patients. A dose of 59Fe is administered orally and the retention of radioactivity is measured on the 14th day, this amount being equivalent to the amount absorbed. In our male group, the mean absorption of inorganic iron was 22.5 ± 8%, and in females it was 43.3 ± 11%. In 10 patients with iron deficiency anemia, the mean was 92.2 ± 6%, in 10 with gastric achylia it was 4.6 ± 4%, and in 12 chronic alcoholics a value of 13.9 ± 7% was f

ISSN:0001-5792    

DOI:10.1159/000207714

出版商:S. Karger AG    

年代:1978

数据来源: Karger