摘要:

A case of acute leukaemia with Ph1-chromosome and monosomy 7 is reported, in which the peripheral blood contained three types of blast cell as distinguished by light and electron microscopy and immunological phenotyping. The first blast-cell type originated from the granulocytic lineage; the cells contained per-oxidase-positive granules, and had an HLA-DR+Tdt-CALLA-phenotype. The second blast-cell type was more difficult to define, but had many characteristics of the monocytic series. The phenotype of these blast cells was HLA-DR+Tdt-CALLA-BA-2+ or HLA-DR+/–TA-1+63D3+. Finally, the third type of blast cell was clearly of lymphocytic origin. These cells were peroxidase-negative, and were CALLA+ as studied by electron microscopy using immunogold labelling and fluorescence microscopy. Their phenotype was HLA-DR+Tdt+CALLA+. Cell sorting and double fluorescence assays showed that these three populations were separate; no cells of mixed myeloid/lymphoid phenotype were found.This case suggests the neoplastic transformation of an immature progenitor cell and subsequent differentiation of the neoplastic cells in various direction

ISSN:0001-5792    

DOI:10.1159/000206304

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The results obtained by using the protein A-rosette assay for human peripheral blood lymphocytes treated with a series of monoclonal antibodies were very close to the estimates obtained by indirect fluorescence. The percentages of T3-, T4-, T8- and DR-positive cells (T3 = T lymphocytes, T4 = helper T lymphocytes, T8 = suppressor T lymphocytes, DR = B, L and null cells) in normal and pathological peripheral blood were found to vary within the limits reported by many other authors using different techniques for the identification of cells binding the specific monoclonal antibody.

ISSN:0001-5792    

DOI:10.1159/000206305

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Ferritin secretion in patients with hematological malignancies, particularly malignant lymphomas, were evaluated in terms of circulating ferritin-secreting cells (FSC). There was a positive correlation between the tumor cell mass and the number of FSC, particularly monocytic FSC, but lymphoma cell suspensions contained very few FSC in comparison with the expected number. These results indicate that lymphoma cells per se may not be responsible for ferritin synthesis and secretion and suggest that ferritin synthesis and secretion in the reticuloendothelial system may be increased as a nonspecific response.

ISSN:0001-5792    

DOI:10.1159/000206306

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Ultrasonically guided fine-needle aspiration biopsy of abdominal focal lesions was performed on 15 patients with non-Hodgkin’s lymphoma, in 14 cases for pathological staging, in 1 case for differential diagnosis between lymphoma recurrence and other malignancy. The biopsy was performed using a transperitoneal approach, guided by a dynamic scanner. A correct diagnosis was obtained in punctures performed on 14 patients (93%); 12 patients had true positive results and 2 had true negative results. One patient had a false negative result; no false positive diagnoses were found. There were no complications related to the biopsie

ISSN:0001-5792    

DOI:10.1159/000206307

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A woman affected by acute myeloblastic leukemia was grafted with HLA A, B and D compatible rhesus-positive bone marrow from her brother. Before grafting, she had anti-D alloantibodies (1/512 IAT, 2.9 μg/ml). To prevent the destruction of donor red blood cells, four plasma exchanges and a conditioning regimen (total-body irradiation 800 rad, cyclophosphamide, methotrexate) were carried out to decrease anti-D from 2.9 to < 0.02 μg/ml on day 0. The anti-D level was 0.8 μg/ml on day 12 and was decreased to 0.2 μg/ml by eight plasma exchanges until day 35. Anti-D antibodies were undetectable with Lalezari’s technique on day 45. Engraftment was obtained on day 25 (3,000 leukocytes/mm3 and 50% erythroblasts in bone marrow). The patient died from aspergillosis and graft-versus-host disease on day 54. This observation shows that an engraftment of rhesus-positive bone marrow in a recipient with anti-D antibody is pos

ISSN:0001-5792    

DOI:10.1159/000206308

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Two pregnancies at risk for Fanconi’s anemia have been monitored by a cytogenetic method in the first trimester of gestation. The rate of chromosome breakage was evaluated in spontaneous mitoses from a direct preparation of trophoblasts in one case and from mitoses obtained from standard cultures and from mitoses treated with diepoxybutane in both cases. Cytogenetic studies were carried out also at 16 weeks from amniotic fluid cells in one case, and in fibroblasts sampled from the aborted fetus in the other. In all the experimental conditions the mean frequency of breaks/cell was in the range of controls, suggesting that the fetuses were unaffected by Fanconi’s anemia. In one case the results have been confirmed by chromosome analysis at bi

ISSN:0001-5792    

DOI:10.1159/000206309

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The cord blood of 1,207 randomly selected neonates from the Cape Coloured population of South Africa was analysed for the presence of Hb Barts. 40 individuals (3.3 %) had detectable Hb Barts levels with values ranging from 1.1 to 7.3%. Restriction enzyme analysis of DNA from subjects with Hb Barts showed that 85% of the cases studied had the genotypes – α3.7/αα r– α3·7/–α3–7. The observed frequency of the –α/αα genotype was much lower than the expected frequency which suggests that this genotype is often not associated with detectable levels of Hb Barts. Mapping of the g-globin locus in three subjects with HbH disease revealed the presence of the – –SEA/α-thalassaemia determi

ISSN:0001-5792    

DOI:10.1159/000206310

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Cytogenetic analysis of bone marrow cells in a 40-year-old man revealed a mosaic of two cell lines during blast crisis: one diploid with typical Ph1 chromosome (46 XY Ph1) and one with hyperdiploidy of 54 chromosomes with double Ph1 chromosome, trisomy 4, 6, 8, 18, 21 and duplication of the sex chromosomes X and Y. Immunological characterization showed common acute lymphoblastic leukemia antigen in 90% of the blasts. In spite of intensive chemotherapy the patient died in a third blast crisis 15 months after the first blast crisis, 3 months after successfully treated central nervous system relapse.

ISSN:0001-5792    

DOI:10.1159/000206311

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

In neutrophils from a 75-year-old woman suffering from chronic leukemia, an abnormal alkaline phosphatase (ALP) was observed, characterized by a significant increase in neutrophil alkaline phosphatase (NAP) activity, associated with an ectopic expression of two isoenzymes: a thermolabile ALP similar to the early placental form (or non-Regan variant), and a heat-stable isoenzyme closely related to the Nagao isoenzyme. The presence of these oncodevelopmental NAP isoenzymes in a myeloproliferative syndrome has previously not been reported.

ISSN:0001-5792    

DOI:10.1159/000206312

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A 61-year-old female patient presented with osteolytic skeletal lesions. Open rib biopsy disclosed tumor tissue which consisted of two types of cells: massive infiltrates of poorly differentiated lymphoplasmacytic cells were surrounded by large deposits of differentiated plasma cells. Serum immunoglobulins were normal. No light chains could be found in the urine. An immunoperoxidase technique was employed to detect intracellular immunoglobulins. While the partially differentiated cells were negative, the plasma cells were stained strongly positive for kappa light chains.

ISSN:0001-5792    

DOI:10.1159/000206313

出版商:S. Karger AG    

年代:1985

数据来源: Karger