摘要:

When leukaemic lymphoblasts from acute lymphatic leukaemia were reacted with animal anti-human lymphocyte globulin (AHLG), and subsequently with the appropriate, fluorescein-isothiocyanate-conjugated antibodies, a brilliant membrane fluorescence was observed, which persisted up to 5 log titres, breaking from rings into spots at higher dilutions. The best results were obtained with fresh suspensions and incident illumination. Cross-reactions with mature and immature myeloid cells with the unabsorbed antisera could be abolished by repeated preabsorbtion of AHLG with packed leukocyte preparations from chronic and acute myeloid leukaemias. Thus, it appears that this approach is susceptible of improving the identification of leukaemic lymphoblasts, and may be extended, by means of appropriate antisera, to the acute leukaemias in general.

ISSN:0001-5792    

DOI:10.1159/000208064

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

In 54 patients with leukaemia a raised incidence of HL-A9 was noted as well as a markedly increased association between this antigen and HL-A2. This occurred most frequently in patients with chronic myeloid leukaemia. As HL-A2 and HL-A9 are both antigens of the first series it has been suggested that the predisposition to develop leukaemia is controlled by a recessive gene closely linked to the first HL-A locus and in a linkage disequilibrium with HL-A2 and HL-A9. 5 patients also showed definite changes between antigens of the same series, whilst others suffered a partial or total loss in antigenicity. Lymphocytes from 145 controls did not behave in this way, though other patients receiving radiotherapy also ‘lost’ antigens. So it was postulated that such changes resulted from the treatment of the disease rather than the disease its

ISSN:0001-5792    

DOI:10.1159/000208065

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

An attempt was made to study preleukemic changes in bone marrow cell proliferation. Seven patients with hypercellular marrows and aregenerative anemia were studied. Five of them could be followed to autopsy, several years after the kinetic studies; all died with a picture of leukemia. Total bone marrow cell numbers, erythroblast generation times, and erythrocyte production were estimated with 59Fe. Despite hypercellularity, the total erythroblast number was not significantly increased. Erythrocyte production and life span were both decreased, and erythroblast generation times were significantly longer than normal.

ISSN:0001-5792    

DOI:10.1159/000208066

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Both hemodialysis and renal allograft recipients have a significantly reduced number of total T lymphocytes per microliter of blood. Simultaneous in vitro lymphocyte responsiveness to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) revealed in the normal subjects, a positive correlation (r = +0.427) between percent T lymphocytes and PHA and a negative correlation (r = -0.525) between percent T lymphocytes and PWM. Such trends were not observed in the hemodialysis patients and transplant recipients. Thus, the enumeration of lymphocytes as T cells appears to provide no clear indication of their functional capacity to respond to mitogenic stimulation in these two categories of patients.

ISSN:0001-5792    

DOI:10.1159/000208067

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The effect of 3 different detergents (Triton X-100, Lubrol WX and Nonidet) on brain thromboplastin was examined and compared with the solubilisation due to deoxycholate. The effect of deoxycholate is superior over that of the other examined detergents. Deoxycholate extracts thromboplastic activity from brain tissue to a large extent. Furthermore it has no interfering absorbance at 280 nm. The aminopeptidase of brain tissue may be separated from thromboplastin. It does not have any coagulation activity.

ISSN:0001-5792    

DOI:10.1159/000208068

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

On the occasion of a double heterozygote case of D haemoglobinopathy /β-thalassaemia (D thalassaemia) from Epirus (Greece), a family study was performed. It comprised 18 members, belonging to 3 generations, and revealed the presence of an additional D thalassaemia case, 4 D haemoglobinopathy heterozygotes, 5 β-thalassaemía heterozygotes and 7 normal persons. The D thalassaemia cases were initially considered as Hb D homozygotes, according to their electrophoretic phenotype; the family study, however, showed the true nature of their stigmata. These patients presented with mild jaundice, splenomegaly and moderate anaemia, while the Hb D heterozygotes were asymptomat

ISSN:0001-5792    

DOI:10.1159/000208069

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Hematological and biochemical findings in a family with hemoglobin (Hb) Beograd interacting with β-thalassemia are presented. Hb Beograd (α2β2121 Gul→Val) was found in 3 members. In two members it interacted with β-thalassemia. These two double heterozygotes had anemia of intermediate severity and splenomegaly. Studies with 51Cr and 59Fe showed a shortened life span of red cells and ineffective erythropoiesis. The abnormal Hb amounted to 86–87%, and Hb F to 5–7%. No Hb A was present. One subject of the family was heterozygous for Hb Beograd. He showed normal clinical and hematological findings. The abnormal hemoglobin was 38%. Four members of the family were heterozygotes for β-thalassemia. The interaction between β-thalassemia and β-chain variants

ISSN:0001-5792    

DOI:10.1159/000208070

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

A female patient with chronic myeloid leukemia in acute phase was found to have trisomy 11 in her bone marrow cells. The contemporary existence of different clones suggests the following clonal evolution: 46, XX→46, XX, Ph′→ 47, XX, Ph′, + ll→48, XX, Ph′, + ll, + Ph′. It is suggested, that the cytogenetic events leading to trisomy C in advanced cases of chronic myeloid leukemia are of a different character than those which often results in trisomy C in other myel

ISSN:0001-5792    

DOI:10.1159/000208071

出版商:S. Karger AG    

年代:1975

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000208072

出版商:S. Karger AG    

年代:1975

数据来源: Karger