摘要:

The effect of L-asparaginase on various acute human lymphoblastic leukemia cell populations was evaluated in an investigation of its mechanism of action with respect to the blast cell cycle and the growth of the leukemic cell population. Immediately following the injection of the drug, the number of cells in S increased, while those in G2 and M decreased. The percentage in G2 showed no appreciable change. Later a destructive effect of L-asparaginase on cells in S phase was noted, together with reduction of the size of the proliferating compartment. Reference is made to recently proposed proliferation kinetic models in acute leukemia in support of the view that the action of the drug may also be directed towards cells in transition from G0 to G1

ISSN:0001-5792    

DOI:10.1159/000208358

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Bone marrow cells of 8 patients with acute lymphocytic leukemia were studied by cytochemical and autoradiographic methods for the synthesis of nucleic acids in the presence or absence of L-asparaginase. The cultures included thymidine for DNA or uridine for RNA metabolism. In the absence of the enzyme the leukemic lymphoblasts reacted more intensely for DNA and RNA synthesis. The addition of the enzyme revealed depressed incorporation of the labeled precursors by the primitive cells

ISSN:0001-5792    

DOI:10.1159/000208359

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Peripheral blood lymphocytes of 18 normal controls and 32 patients with lymphoproliferative disorders were examined for the presence of surface markers. T cell frequency was decreased in chronic lymphocytic leukemia (CLL), Hodgkin’s disease and myeloma; and was normal or decreased in acute lymphocytic leukemia (ALL). Lymphocytes with surface membrane receptors specific for all Ig were markedly increased in lymphosarcoma and cryomacroglobulinemia, extremely decreased in ALL, normal in Hodgkin’s disease; only lymphocytes specific for IgM were increased in CLL. Receptors for C3 were diminished or absent in CLL and ALL. In the remission phase of ALL, EAC and E rosettes returned to normal and slg were almost normal. The approach presented in this article suggests a classification of these diseases in relation to the T and B cell frequency which may be useful for diagnostic and therapeutic implicat

ISSN:0001-5792    

DOI:10.1159/000208360

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

In the serum chromatographies of healthy subjects folic acid activity (FAA) was eluted from the column in the same fractions as α2- macroglobulin, transferrin and albumin, in this order quantitatively. Diphenylhydantoin markedly increased the binding of FAA to serum proteins, especially transferrin. A similar pronounced increase took place in myeloma, hyperthyreosis and hepatitis. In osteomyelitis the binding had decreased clearly in all protein fractions. Whenever the serum FAA had fallen below 3.0 ng/ml, all activity was bound to serum proteins. The chromatographic aberrations in FAA distribution following various pathological conditions, were seen mainly in the transferrin and α2-macroglobulin area. No conclusions can as yet be drawn on the basis of the present study concerning the specificity of these aberratio

ISSN:0001-5792    

DOI:10.1159/000208361

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

15 haemophiliacs including one patient with von Willebrand’s disease and 8 multiple-transfused patients (7 homozygous sickle cell anaemia, 1 refractory anaemia in pregnancy) were examined by the immuno-electro-osmodiffusion (IOD) method for the prevalence of Australia antigen (Au(1)) and antibody among them. No Au(1) antigen or antibody was detected among the haemophiliacs.A 21/2-year-old child with sickle cell anaemia had Au(1) antibody. Possible explanations for these results are giv

ISSN:0001-5792    

DOI:10.1159/000208362

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

A normal 46, XX karyotype was demonstrated in an Indian woman who has 2 approximately equal populations of red cells and of leucocytes. She also has skin mosaicism. She is a normal female and has borne 4 healthy children. The ABO, Ss, Rh and Fy groups of her 2 red cell populations differed, but both were Le(a+b-) although she is a secretor of B and H substances. The relationship between this and the 7 examples of dispermywith blood group mosaicism reported in the literature is discussed briefly

ISSN:0001-5792    

DOI:10.1159/000208363

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Coagulation studies in 2 patients revealed decreased factor IX activity. Family history of factor IX deficiency or bleeding disorder was not present and the past history of the patients did not disclose any evidence of congenital hemorrhagic disorder. They did not require any plasma or plasma product for the control of their bleeding diathesis and decreased factor IX activity returned to normal in a relatively short period of time. Factor IX deficiency was due to a circulating anticoagulant. With corticosteroid treatment, marked clinical improvement occurred in the second patient with some improvement of her coagulation studies, but it did not seem to effect the circulating anticoagulant level in the first patient

ISSN:0001-5792    

DOI:10.1159/000208364

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

A Cuban child carried 2 abnormal slow moving haemoglobins and suffered from severe haemolytic anaemia. Haematological studies of the family and chemical analysis of the abnormal haemoglobins indicated that he was a case of sickle-cell-haemoglobin D disease, which is produced by the interaction between Hb S and Hb D Punjab. This is the first case of Hb D Punjab found in Cuba. The clinical picture of the patient is discussed

ISSN:0001-5792    

DOI:10.1159/000208365

出版商:S. Karger AG    

年代:1973

数据来源: Karger