摘要:

In untreated pernicious anaemia, the binding of folic acid activity (FAA) to protein zone II of the serum (transferrin) had increased distinctly and to zone I (γ-globulin) slightly. A decrease, on the other hand, was noted in zones IV-V (α2-macroglobulin and albumin). During specific treatment, the changes return to normal, with FAA diminishing in zone II and increasing in zones IV-V. There were still some changes present after 7 weeks of treatment, when the anaemia had already been cured. In a pernicious anaemia patient with neuropathy the chromatogram was not much different from normal, and a slight increase in FAA was noted during treatment in protein zones, I, II and I

ISSN:0001-5792    

DOI:10.1159/000208294

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

Plasma from pig, sheep, goat, cattie, horse, rabbit, dog, rat, guinea pig, and chicken was examined for the presence of specific binders for folic acid. It was found that only pig plasma contains unsaturated avid specific binders of folic acid. The maximum binding capacity of pig plasma for folic acid was found to be 14.0–26.5 ng/ml of plasma. These binders have a lower affinity for other folates and folate analogues. They are partly saturated by endogenous folates and they move electrophoretically in veronal buffer, pH 8.6, in an area including β-globulin and most of the γ-globulin fraction. Specific binders of folic acid with high binding capacity were also found in one female sheep out of the 16 tes

ISSN:0001-5792    

DOI:10.1159/000208295

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

In fava bean extracts the proteins were separated and characterized by gel filtration on Sephadex G-25 columns, cellogel electrophoresis and analytical ultracentrifugation. The dried extract and its chromatographic fractions were used to detect serum antibodies to fava beans by passive haemagglutination tests. 75 patients affected by acute favism were studied by this method. Haemagglutination tests were positive in 5 patients (6.6%) at the onset of the haemolytic crisis but a rapid fall of the titres was observed in the subsequent days, β1c/β1A and immunoglobulin levels showed no significant changes. Our results do not support the view that hypersensitivity to fava bean may be involved in the haemolysis of acute favis

ISSN:0001-5792    

DOI:10.1159/000208296

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

Tritiated thymidine was injected into normal rats at 6-hour intervals for 5.5 days, and the labelling of blood monocytes was recorded. Monocytes were identified on the basis of their cytochemical lysozyme activity, demonstrated by lysis of bacteria around the cells. No labelled monocytes were found one hour after the first injection, but a few labelled lysozyme-negative mononuclear blood cells were seen. 58% of the monocytes were labelled on the first day and 100% on the fourth day. Both unlabelled and heavily labelled monocytes left the circulation at random with a half-time of 12–13 h. The grain count data suggest that monocytes are the progeny of at least 2 succeeding precursor cells with generation times not exceeding 1.6 days and that part of the monocytes stay in a storage pool before being released into the bloo

ISSN:0001-5792    

DOI:10.1159/000208297

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

Thymectomy of neonatal mice, which had been treated with Graffi virus, caused an inhibition of the leukemogenesis. The effect was dependent on the hematological character of the primary leukoses used as virus source. The greater the lymphatic proportion in the primary leukosis, the stronger was the inhibitory effect on leukemogenesis. The smallest inhibition of leukemogenesis was observed in thymectomized mice following injection of virus from chloroleukemias.

ISSN:0001-5792    

DOI:10.1159/000208298

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

Folic acid deficiency has been reported to produce a substantial change in the ratio of hemoglobin S to hemoglobin A in the blood of individuals having sickle cell trait. Synthesis of the specific β-chains of these hemoglobins was studied before and after folate antagonism was produced by methotrexate administration. Incorporation of L-leucine-14C into β0 and βSby bone marrow cells was unchanged as a result of the treatment with methotrexate, indicating that synthesis of these globin chains was not selectively affected by the dr

ISSN:0001-5792    

DOI:10.1159/000208299

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

A new G-6-PD variant has been discovered in a 6-year-old boy, who is a carrier of a severe enzyme deficiency, with a typical favism background. The patient is of South Italian ancestry, and the enzymatic anomaly has been transmitted by the maternal grandfather. The enzyme is characterized by an elecrophoretic mobility slightly slower than that of the Mediterranean group variants (nearly 100%) with a normal affinity for G-6-P and NADP. The activity in red blood cells is very low (3% of the normal). The mutant enzyme is very unstable and its optimum pH is slightly biphasic. It shows an increased utilization of deami-no-NADP and 2-deoxyglucose-6-phosphate. Since this variant is different from those previously described, it has been named G-6-PD Toulouse.

ISSN:0001-5792    

DOI:10.1159/000208300

出版商:S. Karger AG    

年代:1974

数据来源: Karger

摘要:

A case of heterozygosis for Hb E (β26 [B 8] glu→lys) in a 39-year-old woman with iron deficiency anaemia and hypothyroidism is described. Iron therapy raised the Hb E content from 17 to 33%. Hb E is frequent in Southeast Asia and occasionally reported in Europe. This case is the first observed in Ita

ISSN:0001-5792    

DOI:10.1159/000208301

出版商:S. Karger AG    

年代:1974

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000208302

出版商:S. Karger AG    

年代:1974

数据来源: Karger