摘要:

The in vitro effect of chloramphenicol (CAP) on ferrochelatase (heme synthetase) activity was tested on bone marrow obtained from patients with and without evidence of CAP toxicity. In the control group of 24 randomly selected patients who had not previously received CAP, inhibition of heme synthesis by CAP (30 μg/ml) was found in 2 and stimulation in 4 instances. In the majority, i.e. 18 patients, heme synthesis was not influenced by CAP. Studies on bone marrow obtained from 8 patients who tolerated CAP without adverse effects revealed stimulation of heme synthesis in 2 instances and inhibition in none. Transient inhibition of ferrochelatase was found in only 1 out of 4 patients recovering from aplastic anemia caused by CAP. However, inhibition of ferrochelatase was repeatedly observed in samples obtained from 3 out of 5 patients with reversible, sideroblastic anemia due to CAP. The intensity and incidence of in vitro alterations of heme synthesis by CAP increased at higher CAP concentrations (300 μg/ml). We conclude that a direct, dose-dependent interference of CAP with ferrochelatase may inhibit heme synthesis in vitro and erythroid iron utilization in susceptible individual

ISSN:0001-5792    

DOI:10.1159/000208534

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

In 42 normal and 65 perinicious anaemia (PA) sera, no significant difference has emerged between the B12 binding capacity. However, the range of B12 binding in PA sera has been found to be much wider, and it is suggested that this may be a source of error in detecting intrinsic factor (IF) antibodies. To eliminate this error, a new modification of the charcoal adsorption method of IF antibody detection is described.

ISSN:0001-5792    

DOI:10.1159/000208535

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Quantitative studies revealed losses of appreciable amounts of iron in 2 patients with the nephrotic syndrome. Increased urinary iron losses amounting to as much as 20 to 100 times the normal values were observed, but the amount of iron excretion did not exceed 0.5 mg/day. Therefore an anaemia cannot be explained by this iron loss. Furthermore, it is postulated that the ratio iron: transferrin in the serum influencesthe urinary iron loss in these patients.

ISSN:0001-5792    

DOI:10.1159/000208536

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

In vitro labelling of circulating blasts with 3H-thymidine was followed by their autotransfusion in a case of steady state acute lymphoblastic leukaemia. Labelling index and mean grain values were then determined autoradiographically at various intervals on both peripheral and marrow blood.The results showed that circulating blasts in S-phase returned to the marrow and then passed back to the circulating compartment after division. Their half-live values was 22.1 h.

ISSN:0001-5792    

DOI:10.1159/000208542

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Polymorphonuclear (PMN) leukocytes from patients with sickle cell anemia and a history of repeated infection failed to show a stimulation of respiratory CO2, O2 consumption, oxidation of formate and reduction of nitroblue tetrazolium during phagocytosis. The abovementioned abnormalities were not found in PMN leukocytes from patients with sickle cell trait, splenectomized patients and normal individuals. The results of this investigation indicate the presence of an additional predisposing factor to infection in some patients with sickle cell anemia.

ISSN:0001-5792    

DOI:10.1159/000208537

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Three cases of hemolytic anemia and pancytopenia with glutathione reductase (GR) deficiency are described. Since flavin adenine dinucleotide is the prosthetic group of GR, riboflavin was administered in order to increase the enzyme activity. Despite the return of the GR activity to normal values, the hemolytic anemia and pancytopenia did not improve. It is conceivable that our cases might represent a latent form of leukemia.

ISSN:0001-5792    

DOI:10.1159/000208538

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

The effect of insulin, glucagon and triiodothyronin (T3) on platelet aggregation was tested. It was shown that insulin and T3 rendered the platelets hyperesponsive to all aggregating agents used. The 2nd phase of aggregation was especially potentiated. Glucagon had an inhibitory effect on platelet aggregation, mainly on the 2nd wave of aggregation with adrenalin. Hypothyroid patients showed abnormal pattern of aggregation which was restored to normal after a week’s treatment with T3

ISSN:0001-5792    

DOI:10.1159/000208539

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

A cytochemical method has been described for the specific and direct staining of glucose-6-phosphate dehydrogenase (G-6-PD) in red blood cells (RBC), by using the tetrazolium salt Nitro-BT (NBT). This method allows a semiquantitative estimation of G-6-PD activity in single cells. The results of such estimations were found to be correlated with spectrophotometric determination of G-6-PD activity in RBC lysates. The activity of G-6-PD was shown to decline with aging of the cell. In cases of severe G-6-PD deficiency, where the spectrophotometric assay revealed no activity, staining of ‘young’ RBC showed appreciable activity, while the ‘old’ cells showed no a

ISSN:0001-5792    

DOI:10.1159/000208540

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Clinical and haematological findings of a Sardinian family are described. Three members were carriers of Hb J Sardegna, 5 were heterozygous for β-thalassaemia and 2 were double heterozygous for Hb J Sardegna and β-thalassaemia. The presence of Hb J Sardegna did not give rise to appreciable changes in the haematological picture: heterozygous carries displayed normal values, while double Hb J/β -thalassaemia heterozygotes presented clinical and haematological signs typical for β -thalassaemia. In the propo-situs, the relative rate of synthesis of «- and β-chains and oxygen dissociation curve were studied. The interaction between β -thalassaemia and α-chain variants is di

ISSN:0001-5792    

DOI:10.1159/000208541

出版商:S. Karger AG    

年代:1972

数据来源: Karger