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1. |
T Lymphocyte Subpopulations in Myelodysplastic Syndromes |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 173-175
Giovanni Carpani,
Alberto Rosti,
Nadia Vozzo,
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摘要:
T cell subpopulations, defined by monoclonal antibodies (OKT3, OKT4 and OKT8), were assessed on 13 patients with myelodysplasia (MDS). The percentage and numbers of OKT3- and OKT4-positive lymphocytes were significantly lower (p < 0.025) than in normal controls, whereas those of OKT8 were not. In the group of patients with refractory anemia with excess of blasts (RAEB) and in those with chronic myelomonocytic leukemia, the percentage and absolute numbers of OKT8 lymphocytes were significantly lower (p < 0.025) than in patients with refractory anemia or with primary acquired sideroblastic anemia, while those of OKT3 and OKT4 did not differ significantly. Quantitative impairment of T cell subpopulations may be part of the myelodysplastic situation as a result of the dyslymphopoiesis, according to the hypothesis that MDS originate from pluripotent stem cells. The decrease of OKT8 in RAEB and chronic myelomonocytic leukemia could be related to the previously shown insufficient erythropoietic activity in these patients.
ISSN:0001-5792
DOI:10.1159/000205556
出版商:S. Karger AG
年代:1989
数据来源: Karger
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2. |
Changes in Peripheral Blood Mononuclear Cell Subpopulations during Antithymocyte Globulin Therapy for Severe Aplastic Anemia |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 176-180
X. López-Karpovitch,
M.E. Zarzosa,
M.R. Cárdenas,
J. Piedras,
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摘要:
The short-term effect of a domestically produced equine antithymocyte globulin (ATG) was analyzed in 6 patients with acquired severe aplastic anemia (AA). All patients received 5 doses of ATG every other day in a 60-min intravenous infusion. Five peripheral blood immunoregulatory mononuclear cell (MNC) subsets, defined by monoclonal antibodies, were enumerated before and 24 h after each application of ATG. Following the first dose of ATG there was a significant and transient reduction in the absolute number of helper T lymphocytes. There was no statistically significant difference pre-ATG to post-ATG in the absolute number of MNC expressing activation antigen Tac (interleukin-2 receptor). However, Tac+ cells, which were significantly increased before ATG therapy, decreased to nearly normal levels after the fourth dose of ATG. A significant and sustained increment in the absolute number of monocytes (CD14+ cells) occurred following the third dose of ATG. The absolute numbers of MNC, suppressor T lymphocytes and cells bearing HLA-DR antigen remain without significant change along ATG treatment. These results suggest that: (a) Tac+ cells are probably involved in the pathogenesis of AA; (b) the target of ATG may be a Ta+ cell, and (c) ATG may stimulate monopoiesis.
ISSN:0001-5792
DOI:10.1159/000205557
出版商:S. Karger AG
年代:1989
数据来源: Karger
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3. |
Beta-2-Microglobulin Is a Reliable Tumor Marker in Chronic Lymphocytic Leukemia |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 181-185
Samuele Di Giovanni,
Gaetano Valentini,
Paolo Carducci,
Paolo Giallonardo,
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摘要:
Serum β2-microglobulin (β2M) was measured in 22 patients with B chronic lymphocytic leukemia (CLL) and in 15 healthy age-matched control subjects. The patients were allocated in stages in accordance with the Rai and Binet systems. The β2M mean value in the CLL group was significantly higher than in the control group. There was a positive correlation between β2M and the clinical stage in both staging systems. The mean serum levels of β2M in stages A, B and C of Binet’s system showed statistical differences while in stage A they did not differ from those found in the control group. No correlation was demonstrated between β2M and the peripheral lymphocyte concentration. A slight correlation was found between β2M and the degree of bone marrow lymphocyte infiltration, whereas a stronger relationship was observed when the type of infiltration (non-diffuse versus diffuse) was considered. Patients with bulky disease had mean β2M values much higher than t
ISSN:0001-5792
DOI:10.1159/000205558
出版商:S. Karger AG
年代:1989
数据来源: Karger
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4. |
Hypersegmented Neutrophils and Vitamin B12Deficiency |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 186-191
Warren G. Thompson,
Cara Cassino,
Lisa Babitz,
Thomas Meola,
Russell Berman,
Mack Lipkin, Jr.,
Michael Freedman,
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摘要:
The sensitivities and specificities of the mean cell volume (MCV), the red cell distribution width (RDW), and blood smear hypersegmentation for B12 deficiency were reviewed in 515 patients whose B12 levels were determined. 61 patients had B12 levels less than 200 pg/ml. 43 patients were defined as B12 deficient (n = 13) or non- B12 15% (54%) in detecting B12 deficiency. The MCV and the RDW should not be relied on when screening for B12 deficiency; examination of the blood smear for hypersegmentation is essential.
ISSN:0001-5792
DOI:10.1159/000205559
出版商:S. Karger AG
年代:1989
数据来源: Karger
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5. |
Primary (Essential) Thrombocythemia versus Initial (Hyperplastic) Stages of Agnogenic Myeloid Metaplasia with Thrombocytosis – A Critical Evaluation of Clinical and Histomorphological Data |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 192-202
Jürgen Thiele,
Rudolf Zankovich,
Thomas Steinberg,
Barbara Kremer,
Robert Fischer,
Volker Diehl,
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摘要:
In 40 patients (17 male, 23 female, median age 57 years) with the presumptive diagnosis of primary (essential) thrombocythemia (PTH) according to the diagnostic requirements of the Polycythemia-Vera- Study-Group (PVSG) a follow-up study and a histological evaluation of initial trephine biopsies of the bone marrow were performed. Thorough review of the hematological data during the lengthy course of disease (observation time ranging from 1.5–10.5 years) and the histomorphology of the bone marrow implied a discrimination into two groups of patients. Group I patients (n = 26; 10 male, 16 female) were compatible with PTH according to our follow-up studies. Group II patients consisted of 14 cases (7 male, 7 female) which suggested retrospectively early hyperplastic stages of agnogenic myeloid metaplasia (AMM) with concomitant thrombocytosis. In PTH (group I patients) there was a sustained elevation of the platelet count lasting for several years accompanied by stable other blood values. Early AMM (group II patients) was characterized by an insidious decline of the initially elevated thrombocyte count, starting in a few patients already 4–6 months after admission. In AMM there was further an increase in hepatosplenomegaly observable together with the level of LDH and the score of the leukocyte alkaline phosphatase, and finally an evolution of a leukoerythroblastic blood picture could be noticed. Initial histopathology of the bone marrow revealed a profound proliferation of a not severely dysplastic megakaryopoiesis in group I patients (PTH) and a normal content of reticulin fibers. In early thrombocythemic AMM (group II patients) conspicuous abnormalities of megakaryocytes were accompanied by a slight to moderate increase in argyrophilic fibers and a left-shifted neutrophilic granulocyto as well as erythropoiesis. These differences of certain histomorphological features could be substantiated by morphometric analysis. Our findings suggest that even the rigid requirements for the diagnosis of PTH as proposed by the PVSG may not be sufficiently restrictive to exclude patients with early hyperplastic stages of thrombocythemic
ISSN:0001-5792
DOI:10.1159/000205560
出版商:S. Karger AG
年代:1989
数据来源: Karger
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6. |
Vacuolated Plasma Cell: Ultrastructural Distribution of Acid Phosphatase and Intracellular Immunoglobulin |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 203-207
Norimasa Yasuda,
Tadashi Kanoh,
Haruto Uchino,
Kazuo Ogawa,
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摘要:
In two cases of vacuolated plasma cells, one of which was associated with primary macroglobulinemia and the other was with κ-chain Bence Jones multiple myeloma, we examined the immunopathological features of the vacuoles in order to know whether the Ig-secreting system or the lysosomal system is of importance in the process of vacuole formation. Immunofluorescent studies detected no Ig in the vacuoles. Detection of intracellular Ig by immunoelectron microscopic technique revealed that Ig was localized only in a small portion of the vacuoles but not in most vacuoles. Even when Ig was included in the vacuoles, only the contents of the vacuoles were positive for Ig but their demarcating membrane was negative for Ig. In contrast, electron microscopic studies of acid phosphatase activity revealed the presence of its activity in all vacuoles. These findings suggest that the lysosomal system but not the Ig-secreting system may play a major role in vacuolation of these myeloma cells
ISSN:0001-5792
DOI:10.1159/000205561
出版商:S. Karger AG
年代:1989
数据来源: Karger
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7. |
Aspirin-Induced Acute Haemolytic Anaemia in Glucose-6-Phosphate Dehydrogenase-Def icient Children with Systemic Arthritis |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 208-209
Tullio Meloni,
Gavino Forteleoni,
Augusto Ogana,
Vannina Franca,
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摘要:
In a 4-year 7-month-old boy with glucose-6-phosphate dehydrogenase deficiency and systemic arthritis a severe haemolytic anaemia occurred after the administration of acetylsalicylic acid. Erythrocyte fragmentation, with haemoglobin condensation zones next to clear zones, was observed on peripheral blood smears. Since viral or bacterial infections were excluded on the basis of the laboratory data, the anaemia was ascribed to aspirin.
ISSN:0001-5792
DOI:10.1159/000205562
出版商:S. Karger AG
年代:1989
数据来源: Karger
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8. |
In vivo Dose-Response Effect of Recombinant Human Granulocyte Colony-Stimulating Factor on Increase in Granulocytes after Peripheral Blood Stem Cell Autotransplantation |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 210-212
Yoichi Takaue,
Tetsuya Koyama,
Tsutomu Watanabe,
Yoshifumi Kawano,
Tsuneo Ninomiya,
Yasuhiro Kuroda,
Akira Hiraoka,
Tohru Masaoka,
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摘要:
The in vivo dose-response potential of recombinant human granulocyte colony-stimulating factor (rG-CSF) in facilitating reconstitution of granulopoiesis was evaluated in an 11-year-old girl who received autotransplantation of peripheral blood stem cells (PBSC) after marrow-ablative therapy for relapsing acute lymphoblastic leukemia. The recovery of hematopoiesis was slow when a small number of progenitors were collected and reinfused into the patient, but administration of rG-CSF led to significant dose-dependent increase in peripheral blood granulocytes.
ISSN:0001-5792
DOI:10.1159/000205563
出版商:S. Karger AG
年代:1989
数据来源: Karger
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9. |
Circulating Mononuclear Cells from Pure Red Cell Aplasia of Chronic Lymphocytic Leukemia Suppress in vitro Erythropoiesis |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 213-216
Zeev Estrov,
Alain Berrebi,
Gustavo Kusminsky,
Peretz Resnitzky,
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摘要:
In vitro studies were performed in a patient with B-cell chronic lymphocytic leukemia who developed pure red cell aplasia (CLL-PRCA). The patient’s irradiated circulating mononuclear blood cells and supernatant markedly inhibited normal marrow erythroid (but not granulocyte-monocyte) progenitor colony proliferation. In contrast, irradiated peripheral blood mononuclear cells and supernatant obtained from a B-CLL patient (Rai stage III) and from a hematologically normal donor, did not affect hematopoietic progenitor colony growth. These findings suggest that the anemia of CLL-PRCA evolves different mechanisms of those causing anemia in CLL, and is mediated through cellular and secretory mechanism
ISSN:0001-5792
DOI:10.1159/000205564
出版商:S. Karger AG
年代:1989
数据来源: Karger
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10. |
Philadelphia Chromosome (Ph1-Positive Acute Lymphoblastic Leukemia (ALL) Is Resistant to Effective Therapy for Ph1Negative ALL |
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Acta Haematologica,
Volume 81,
Issue 4,
1989,
Page 217-218
Zalmen A. Arlin,
Eric J. Feldman,
Tauseef Ahmed,
David Liebowitz,
Lawrence R. Shapiro,
Patrick L. Wilmoi,
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摘要:
Amsacrine with high-dose cytarabine is effective therapy for Philadelphia chromosome (Ph1-negative acute lymphoblastic leukemia (ALL). We examined the effectiveness of this regimen in 19 patients with Ph1-positive lymphoblastic leukemia. Four had an antecedent chronic phase of chronic myelogenous leukemia and 15 presented with ALL. There were no complete responders in either group. All 14 patients whose bone marrow could be assessed after completion of therapy showed persistent leukemia. We conclude that patients with Ph1-positive lymphoblastic leukemia have a disease that is resistant to treatment that is highly effective in patients with Ph1-negative ALL.
ISSN:0001-5792
DOI:10.1159/000205565
出版商:S. Karger AG
年代:1989
数据来源: Karger
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