摘要:

Autotransfusion of 3H-cytidine-labelled blood lymphocytes followed by autoradiographic evaluation as studied in 11 patients with Hodgkin’s disease and 3 other patients seems to be an appropriate method to estimate migration kinetics and pool sizes of lymphocytes. However, it is complicated by two major pitfalls: (1) The free (extracellular) activity of 3H-cytidine transfused along with the cell-bound label is sufficient to cause DNA labelling of newly produced lymphocytes in the body. (2) The soluble intracellular pool of 3H-labelled compounds causes an increase of labelling intensity of lymphocyte RNA in the first hours after cell transfusion. Methods to correct for these sources of error are describe

ISSN:0001-5792    

DOI:10.1159/000208184

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The dilution in the circulating blood of lymphocytes labelled in vitro with 3H-cytidine was examined after autotransfusion in 9 patients with Hodgkin’s disease (HD) stage II A-IV B, 5 of whom were untreated; in 2 untreated patients with carcinoma, and in 1 treated patient with scleroderma. The blood transit time of exchangeable lymphocytes was 37+18 min in the patients with HD and 26+6 min in the other patients. The proportion of exchangeable (recirculating) small blood lymphocytes was 39–84% in HD and 81-91% in the carcinoma patients. The relation between the size of the circulating pool of small blood lymphocytes and the total exchangeable (recirculating) lymphocyte pool was 1:20 to 1:30 in HD and 1:29 to 1:34 in the other patients. The absolute size of the recirculating pool of lymphocytes was 46-90 × 109 cells in HD and 100-150 x109 cells in the carcinoma pati

ISSN:0001-5792    

DOI:10.1159/000208185

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The cellular distribution of intravenously administered 3H-heme, 125I-hemopexin and 125I-albumin was studied in rabbits. Radioautography of the tissue slides of several organs was examined by light and electron microscopy. All experiments were terminated 60 min after injection of the isotope-labeled materials. 3H-heme and native monomeric 125I-hemopexin (heme saturated) were found to be associated with the endoplasmic reticulum and microbodies of the hepatocytes. Aggregated hemopexin was also taken up by macrophages of lung alveoli, splenic pulp, interstitial cells of the kidney and Kupffer cells. 125I-albumin (heme saturated) could not be located in liver, spleen, bone marrow, lung, or kidney tissues.

ISSN:0001-5792    

DOI:10.1159/000208186

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Hematopoietic stem cells (CFU) found in the peripheral blood of mice might be, functionally, a heterogenous cellular population. A comparison of our data with that of others leads to the conclusion that peripheral blood CFU belong to two or more marrow CFU subpopulations, one of which consists of erythrocytic committed CFU.

ISSN:0001-5792    

DOI:10.1159/000208187

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

A 53-year-old dock labourer, presented with massive splenomegaly and subsequently developed pancytopenia. Complete haematological remission was observed following splenectomy. Histological examination of the liver and spleen revealed sarcoid granulomata. A year after splenectomy, he died in an acute haemolytic crisis, a very rare complication of sarcoidosis. Evidence of generalized sarcoidosis was found at autopsy. The literature on haematological complications in sarcoidosis is reviewed.

ISSN:0001-5792    

DOI:10.1159/000208188

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The authors report a case of acute myelo-monocytic leukaemia occurring in a patient, 3 years after the beginning of a typical paroxysmal nocturnal haemoglobinuria (PNH). An intermediate phase characterized by worsening of the anaemia was observed, with disappearance of the in vitro haemolysis tests. The kinetic studies showed a replacement of the early peripheral haemolysis by a bone marrow insufficiency with intra-medullary cell death. A review of the five previous reported cases of this association show that PNH is generally a typical one, so that acute leukaemia must be considered as an unfrequent but possible complication of any PNH.

ISSN:0001-5792    

DOI:10.1159/000208189

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

We describe a case of IgM paraproteinemia, whose macroglobulin reacted anomalously with heparin. Anti-heparin activity of the paraprotein was suggested by insensibility of the patient’s plasma to heparin in heparin-thrombin clotting time. Moreover, the presence of heparin in the medium markedly reduced the inhibitory effect of the paraprotein on platelet aggregation by AD

ISSN:0001-5792    

DOI:10.1159/000208190

出版商:S. Karger AG    

年代:1975

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000208191

出版商:S. Karger AG    

年代:1975

数据来源: Karger