摘要:

HLA-A and HLA-B antigens were determined in 16 unrelated subjects orginating from Veneto affected by idiopathic haemochromatosis (IH). HLA-A3 was found in 13/16 patients vs. 300/1,348 controls (p < 0.00005). Prevalences of A3, B35 haplotype were 0.4375 in patients vs. 0.0816 in controls (p < 0.0005). Linkage disequilibrium analysis proved the existence of a positive third-order linkage disequilibrium among IH, HLA-A3 and HLA-B35 alleles. Our data confirm the close association of IH and HLA-A3 and prove the peculiar association of the disease with A3, B35 haplotype in north-eastern regions of Italy. The positive third-order linkage disequilibrium suggests a remote event (mutation, recombination or immigration) as origin for IH and A3, B35 association.

ISSN:0001-5792    

DOI:10.1159/000205184

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Twenty-three patients admitted for treatment of refractory or relapsed acute leukemias (12 acute nonlymphocytic leukemias, 4 acute lymphocytic leukemias, 1 acute undifferentiated leukemia, 6 chronic myeloid leukemias in blast crisis) received a regimen employing a 4-day continuous intravenous infusion of epirubicin followed by a 4-day continuous infusion of vincristine. The remission rate (complete and partial) was 52%. This treatment was associated with minimal extrahematologic toxicity, particularly cardiac and gastrointestinal toxicity. This method of administration should be considered a reasonable therapeutic approach to pretreated acute leukemia patients, particularly in elderly patients wishing to avoid chemotherapy-related side effects.

ISSN:0001-5792    

DOI:10.1159/000205185

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The records of 48 patients with homozygous β -thalassemia were reviewed for evidence of cholelithiasis by abdominal ultrasonography or plain abdominal X-ray. The presence of cholelithiasis was reported in 25 patients (52%). The incidence increased with age to 83% in patients over 31 years of age. Gallstones were more frequent among patients with β-thalassemia intermedia and with less blood transfusion requirements. Eleven patients with cholelithiasis (44%) became symptomatic. Eight received operations on an elective or semielective basis. Another patient had incidental cholecystectomy during splenectomy. The preoperative evaluation included abdominal ultrasonography, nucleotide biliary scan and evalulation of the cardiopulmonary status. Due to the longer survival of patients with homozygous β-thalassemia, the problem of cholelithiasis is becoming more freque

ISSN:0001-5792    

DOI:10.1159/000205186

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The immunological phenotypes of lymphocytes and myeloma cells in 48 patients with multiple myeloma (MM) were analyzed using a panel of monoclonal antibodies (mAbs). Myeloma cells were positive for OKT10, BL3, PCA1 and BA2. In a few cases, they were also positive for the B cell-associated antigens J5, B1 and I2. Eight of 48 cases had more than 15% J5-positive lymphocytes, and some lymphocytes in MM expressed plasma cell-associated antigens (PCA1, BL3, OKT10), suggesting a possible clonal involvement. These observations demonstrate the heterogeneity of surface antigen expression of myeloma cells and suggest that BL3, PCA1, BA2 and J5 may be useful mAbs for purging myeloma cells from bone marrow for autologous transplantation.

ISSN:0001-5792    

DOI:10.1159/000205187

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Levels of plasma selenium (Se) and glutathione peroxidase were measured in 20 sickle cell anemia (SCA) patients not in crisis and in 14 nonanemic control subjects. The results show that the levels of Se and glutathione peroxidase were significantly (p < 0.005) lower than those of controls in both plasma and whole blood. These data are consistent with the previous reports that there is increased oxidative stress in SCA. Low blood Se levels and glutathione peroxidase activity observed in this research suggest that a weakened antioxidant potential may be associated with SCA patients. The low Se status in SCA patients may also affect the phenotypic expression of these patients.

ISSN:0001-5792    

DOI:10.1159/000205188

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The effects of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) on the cell growth of the myeloma cell line KHM-1A were studied. TNF-α strongly induced KHM-1A proliferation, while IL-6 weakly enhanced growth only at low cell densities. When TNF-α was combined with IL-6, TNF-α-induced growth enhancement was reduced. According to Northern blotting analysis, the m-RNA of both TNF-α and IL-6 were detected in KHM-1A. Moreover, monoclonal antibody capable of neutralizing the cytotoxic activity of TNF-α inhibited the proliferation of this cell line. These findings suggest that this cell line operates under an autocrine growth mechanism with respect to these two cytokines, IL-6 and

ISSN:0001-5792    

DOI:10.1159/000205189

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Two Chinese patients with hemoglobin (Hb) H disease without a history of blood transfusion developed iron overload at the age of 45 and 53 years, respectively. Human leukocyte antigen (HLA) system types of these 2 patients were A19, A11, B13, B62 and A2, A24, BW55, respectively, which are not related to the common haplotypes for idiopathic hemochromatosis. Since severe iron loading is a rare clinical manifestation in untransfused patients with Hb H disease, the iron overload in both patients may be due to environmental or underlying genetic factors.

ISSN:0001-5792    

DOI:10.1159/000205190

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

A 29-year-old female in the 20th week of pregnancy was admitted because of a change in the ABO blood group and bleeding tendency. Acute myelogenous leukemia was diagnosed with a weak reaction of red blood cells with anti-A antibody and a decreased level of A-transferase activity. Though the patient tolerated intensive chemotherapy and achieved complete remission, thrombocytopenia persisted after consolidation chemotherapy. Since platelet-associated IgG was elevated, thrombocytopenia was considered to be immunemediated. In the third trimester, premature separation of the normally implanted placenta developed and cesarean section was performed. The male baby was also thrombocytopenic, but successfully treated with γ-globulin

ISSN:0001-5792    

DOI:10.1159/000205191

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

To investigate the origin of cells observed in acute myelomonocytic leukemia with eosinophilia (M4E0), we carried out morphological and cytogenetic studies of colonies derived from leukemic precursors. Marrow cells from a patient with M4E0 at second relapse revealed an abnormal karyotype; 47, XY, +8, inv(16)(p13q22), 17p-. Cytogenetic studies of leukemic colonies showed the same karyotypic abnormality. The primary and secondary colonies consisted of monocytes, eosinophils and basophils. These results indicated that the neoplastic process in this patient originated in a stem cell capable of differentiation into at least monocytes, eosinophils and basophils.

ISSN:0001-5792    

DOI:10.1159/000205192

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

A patient whose chronic myelogenous leukemia (CML) was treated with interferon alpha (IFN-α) is described. The disease showed karyotypic evolution during the chronic phase and the later myeloid acceleration. Both of these secondary clonal phenomena responded to IFN-α dose escalation. The case illustrates the dose dependence of CML responses to IFN-α. The phenomenon of clonal evolution is discussed in the context of this patient’s dis

ISSN:0001-5792    

DOI:10.1159/000205193

出版商:S. Karger AG    

年代:1990

数据来源: Karger