摘要:

Granulocytes were harvested from hematologically normal individuals using continuous flow centrifugation (CFC) or filtration leukapheresis (FL). The isolated granulocytes were labeled in vitro by 3H-diisopropyl fluorophosphate (3H-DFP) and autotransfused. Their intravascular fate was analyzed by autoradiography. Immediately after autotransfusion the majority of granulocytes administered, collected in the marginal granulocyte pool. Margination was particularly prominent in granulocytes isolated by FL. The distribution of transfused granulocytes between the circulating and the marginal granulocyte pool showed wide and irregular fluctuations in time. Margination of transfused granulocytes was counterbalanced, and its fluctuation between the two intravascular pools was stabilized by prednisone treatment. The transit of transfused granulocytes from blood to tissue seemed to be governed by a random process, the half-disappearance time being either normal or prolonged. Compatible granulocytes administered to hematologically normal recipients circulated for at least 20 h.

ISSN:0001-5792    

DOI:10.1159/000208014

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

Five cytochemical reactions in current use (MPO, PAS, LAP, SB and EST) were operated on 180 children aged 1 month to 6 years, divided into three age groups (less than 12 months, 1–3 years and 3–6 years). No significant differences are apparent from one group to another, and the mean values are identical with those of adults. On the other hand, certain variations are evident in comparison with the scores of newborn children: important decrease in LAP, increase in SB, EST and PAS. These tests should be done in investigations of inborn metabolic errors, chromosome anomalies, blood diseases, and defects in defense mechani

ISSN:0001-5792    

DOI:10.1159/000208015

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

In ductus thoracicus lymphocytes of the rat the localization of specific phosphatases was studied by means of ultrahistochemistry. Thiaminepyrophosphatase was found in the outer lamellae of the Golgi-field as well as in lysosomes, whereas nucleosiddiphosphatases could be localized on the plasma membrane at low pH. These findings are correlated with the phaenotype of B- and T-lymphocytes. Their functional significance is discussed.

ISSN:0001-5792    

DOI:10.1159/000208016

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

In 33 children with cyanotic congenital heart disease the platelet function has been studied. The most significant changes were reduced platelet adhesiveness to glass and impaired availability of platelet factor 3 in nearly 50% of the patients. Although clot retraction was poor in 84% of them, thrombocytopenia and prolonged bleeding time were not significant features.

ISSN:0001-5792    

DOI:10.1159/000208017

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

Growth of haematopoietic cells from fetal mouse liver of 14- to 16-day gestation was studied with the diffusion chamber technique. The culture period varied from 1 to 8 days. Cell proliferation and haemoglobin synthesis were seen in erythroblasts during the first days of culture. However, the nearly pure erythroblastic population of the primary inoculum changed to vigorously growing granulocytic cells and macrophages during the 8-day assay period. It seems likely that end-product inhibition is operative within the monocyte-macrophage and granulocyte cell lineages, respectively, when cells grow in diffusion chambers in vivo.

ISSN:0001-5792    

DOI:10.1159/000208018

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

The levels of components of the fibrinolytic enzyme system in 7 patients with anorexia nervosa were compared with those of healthy young women. Plasminogen activator levels were significantly higher and the mean fibrinogen concentration was lower in the anorexia nervosa patients.

ISSN:0001-5792    

DOI:10.1159/000208019

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

A patient with combined factor V and factor VIII deficiency is presented. The bleeding manifestations were mild. The main laboratory feature was a prolonged partial thromboplastin time which was corrected by the addition of adsorbed normal plasma but not by the addition of normal serum, hemophilia A plasma or plasma of another patient with combined factor V and factor VIII deficiency. TGT was also clearly abnormal and was corrected by the addition of adsorbed normal plasma but not by the addition of normal serum. Prothrombin consumption was mildly defective. The prothrombin time was slightly prolonged. Factor VIII was 12% and factor V 55% of normal. Factor- VIII -associated antigen was normal. The father and a sister of the propositus revealed mild factor V deficiency but normal factor VIII activity and antigen. The parents were not consanguineous. A tentative classification of combined deficiency of factors V and VIII in two groups is proposed. The hereditary transmission of the two types of deficiencies is discussed.

ISSN:0001-5792    

DOI:10.1159/000208020

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

Brucellosis was diagnosed in two polytransfused, thalassaemic patients who had received blood containing brucella agglutinins from an asymptomatic donor. In one of the patients the onset of the disease simulated infectious mononucleosis. Despite the fact that both cases were splenectomized, the course of illness was not life threatening. However, a tendency for recurrence of the infection and persistence of high brucella agglutinin titres were noted.

ISSN:0001-5792    

DOI:10.1159/000208021

出版商:S. Karger AG    

年代:1976

数据来源: Karger

摘要:

The simultaneous occurrence of two serum and urine M components in a 75-year-old woman affected by Hodgkin’s disease is reported. The patient developed two IgG-M components of different light-chain types after an episode of pneumococcal pneumoni

ISSN:0001-5792    

DOI:10.1159/000208022

出版商:S. Karger AG    

年代:1976

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000208023

出版商:S. Karger AG    

年代:1976

数据来源: Karger