摘要:

We have studied the growth of erythroid bursts in methylcellulose cultures from bone marrow (7 cases) and peripheral blood cells (17 cases) of patients with primary acquired refractory anemias. In most cases, erythroid burst-forming units (BFU-E) were either absent or present in lower than normal numbers, and these levels remained low to absent in sequential studies. In 2 patients, circulating BFU-E were initially higher than normal, but subsequently declined to normal levels. 1 patient had low-normal numbers of circulating BFU-E initially, but these declined to zero in a later study. No clinical or routine hematological features permitted distinction between patients with initially high BFU-E and those with initially low or absent BFU-E. These findings parallel those reported for granulocyte-macrophage progenitors in refractory anemias, and support the concept that erythroid progenitor cells in these disorders are influenced by the disordered hematopoiesis.

ISSN:0001-5792    

DOI:10.1159/000206682

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Colony formation in vitro from bone marrow haemopoietic progenitors was studied in a group of patients with acute myeloblastic leukaemia and acute lympho-blastic leukaemia at presentation of the disease and, in a few cases, during complete remission. Both granulocytic-macrophagic (CFU-GM) and erythropoietic (CFU-E) colonies were studied. A sharp contrast was observed between CFU-GM and CFU-E formation at presentation of the disease: while the former was markedly depressed, with considerable increase of the cluster colony ratio, CFU-E production was not significantly affected, with only a reduced sensitivity to low-dose erythropoietin. CFU-GM formation returned to normal in the early stages of complete remission, but showed a progressive decline in the course of time; the process of cell differentiation was not significantly impaired, although minor changes were observed. It appears that the leukaemic process has much greater impact altogether on CFU-GM than on CFU-E colony formation, the latter being only marginally affected, even in the presence of a high proportion of blast cells.

ISSN:0001-5792    

DOI:10.1159/000206683

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Total and differential leucocyte countings, mobilization of the marrow granulocyte reserve with hydrocortisone and growth of blood colony-forming cells (CFU-C) in agar culture have been evaluated in 17 patients with Graves’ disease before therapy. Moreover, the myeloid differentiation of blood CFU-C in liquid culture has been studied in 4 patients. Neutropenia was found in only 1 patient (5.9%), while the marrow granulocyte reserve was reduced in 7 out of 15 patients (46.7%). Blood concentration of CFU-C and myeloid differentiation in liquid culture were normal in all patients studied. These data show an abnormal granulopoiesis in a high percentage of patients with untreated Graves’ disease. The mechanisms accounting for the reduction of the marrow granulocyte reserve in patients with Graves’ disease are disc

ISSN:0001-5792    

DOI:10.1159/000206684

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

A human lymphoblastoid cell line (Raji) developed macrocytosis after 5–7 days when cultured in the presence of 100, 250 and 500 mg ethanol/dl. The degree of macrocytosis was least with 100 mg/dl and greatest with 500 mg/dl. The macrocytosis was associated with a proportionate increase in the total protein content per cell, was reversed after culture in the absence of ethanol and was uninfluenced by the supplementation of the culture medium with 50 μg folic or folinic acids per millilitre. Ethanol also caused a substantial prolongation of the cell doubling time at concentrations of 250 and 500 mg/dl (but not 100 mg/dl) and this was associated with some increase in the proportion of non-viable cells in the cultures. Furthermore, ethanol increased the incorporation of 3H-leucine into protein per femtolitre of cell volume. It is proposed that the etha-nol-induced macrocytosis may have developed as a consequence of the stimulation of the rate of protein synthesis within a normal or prolonged cell cyc

ISSN:0001-5792    

DOI:10.1159/000206685

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Proteolytic activity against native hemoglobin polypeptide chains is demonstrated, under strictly physiological conditions, in human reticulocytes of both normal subjects and individuals suffering from a variety of pathologic conditions involving erythrocytes, including β-thalassemia. Two thirds of the activity are found in the cytoplasm and the remainder of it is associated with the reticulocyte membrane. That this proteolytic activity is due to contamination by WBC is excluded. The activity preferentially degrades the α-hemoglobin chains. An increase in this substrate within the eryth-roid cells, as observed in β-thalassemia, does not enhance proteolysis. Protease inhibitors produce a variable decrease in proteolysis. None inhibit completely, thus showing that several enzymes, with different specificities, are involv

ISSN:0001-5792    

DOI:10.1159/000206686

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

We describe 2 elderly patients with splenomegaly and progressive bone marrow failure due to infiltration by leukemic prolymphocytes with a single prominent nucleolus. In each case the leukemia cells had a unique helper T cell phenotype with complement receptors and contained coarse blocks of acid alpha-naphthyl butyrate. A 14q+ chromosome was among the abnormalities that marked one abnormal T cell clone. Histology of the spleen showed mainly red pulp infiltration merging with periarteriolar regions and different from the pseudonodular pattern described in many cases of B cell prolymphocytic leukemia (PLL). Ultrastructurally, leukemia cells contained dense lyso-somes, perinuclear clusters of 10-nm microfilaments but no distinctive cytoplasmic inclusions previously described in (B cell) PLL. We suggest that early phenotyping of leukemia cells may enable consideration of monoclonal antibody therapy in elderly patients with bone marrow failure resistant to chemotherapy.

ISSN:0001-5792    

DOI:10.1159/000206687

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Of 34 patients with chronic inflammatory bowel disease (CIBD), 4 developed well-documented episodes of deep-venous thrombosis. All 4 patients had active disease at the time of thrombosis. This group was studied to determine if the tendency to deep-venous thrombosis in patients with CIBD was associated with reduced antithrombin activity by measuring the concentration of three thrombin inhibitors, antithrombin III (AT III), α2-macroglobulin (α2M) and α1-antitrypsin. 2 patients had low AT III levels and 10 had low α2M levels. 2 patients who developed deep-venous thrombosis had significantly low levels of both AT III and α2M. It is suggested that in patients with diseases predisposing to thrombosis and associated with low AT III levels, the measurement of α2M in addition to AT III may predict those particularly at

ISSN:0001-5792    

DOI:10.1159/000206688

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

A case is reported of an elderly patient with generalized lymphadenopathy who, at presentation, showed a blood and bone marrow picture suggestive of plasma cell leukemia, polyclonal hypergammaglobulinemia restricted almost exclusively to IgA, and severe renal insufficiency. Treatment with melphalan and prednisone produced a complete remission that lasted only 1 month. A second partial remission was obtained, but the patient eventually died of heart failure. The pathological picture of the lymph nodes and spleen was intermediate between that of angioimmunoblastic lymphadenopathy (AIL) and of the plasma cell type of giant lymph node hyperplasia (Castleman’s disease). The hypothesis is presented that AIL, Castleman’s disease and many other polyclonal lymphadenopathies recently described in immunodeficient or elderly patients, including the present case, represent a continuous spectrum of lymphoprolifer-ative disorders due to abnormal responses to various stim

ISSN:0001-5792    

DOI:10.1159/000206689

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

The use of splenic radiotherapy in the treatment of hairy-cell leukaemia is discussed. Reference is made to the case of an elderly female whose cardiorespiratory state precluded splenectomy, and who responded well to splenic irradiation on two occasions.

ISSN:0001-5792    

DOI:10.1159/000206690

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

A case of idiopathic severe aplastic anemia with spontaneous complete remission is described. Hematologic parameters normalized spontaneously 94 days after onset. However, the ability of the patient’s bone marrow cells to form granulocytic-macrophagic colony-forming units (CFU-GM) or erythroid burst-forming units (BFU-E) was depressed until the 288th day, in spite of the normalization of blood counts. Incubation of the patient’s bone marrow cells with antilymphocytic globulin prior to the culture experiment normalized the number of CFU-GM and BFU-E in correlated studies. Coculture of a target marrow with several concentrations of the patient’s lymphocytes or serum resulted in a complete inhibition of CFU-GM. BFU-E growth was inhibited by the patient’s lymphocytes, but not by serum, which rather showed burst-promoting activity. The inhibitory effect on target bone marrow persisted for 288 days, when it disappeared concomitantly with the restoration of spontaneous CFU-GM and BFU-E

ISSN:0001-5792    

DOI:10.1159/000206691

出版商:S. Karger AG    

年代:1983

数据来源: Karger