摘要:

T, B and Null lymphocyte-enriched subpopulations were isolated by means of sheep red blood cell (E SRBC) sedimentation and nylon wool adherence. In these subsets the proportion of Fc receptor-bearing cells was determined by the antibody-coated human and ox erythrocyte rosette assays (EA Hu and EA Ox). In the T-cell fraction the proportion of Fc-bearing lymphocytes was dependent on the procedure by which the sheep erythrocytes were removed from the E SRBC rosette-forming cells. Mechanical vibration resulted in considerably higher percentages of EA rosette-forming cells (EA-RFC) than osmotic shock or lysis with ammonium chloride. In all three procedures the number of EA Hu-RFC was slightly higher than the number of EA Ox-RFC.In the B-cell fraction the proportion of EA Ox-RFC was higher than that of EA Hu-RFC, mean values 43 and 33%, respectively. In the Null cell fraction the reversed was seen: mean values 54% EA Hu-RFC and 45% EA Ox-RFC. In all three lymphocyte fractions the majority of the EA-RFC contained parallel tubular structures and/or associated amorphous granules. Non-Fc receptor-bearing lymphocytes only occasionally showed parallel tubular structures or the amorphous granules.

ISSN:0001-5792    

DOI:10.1159/000207069

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Twenty-two haematological parameters were measured in 30 patients with systemic lupus erythematosus (SLE) at 110 patient attendances. Using a 10,000 cells per sample automated differential counter, the major abnormalities demonstrated were: lympho-penia, monocytopenia, eosinophilopenia (each of which showed strong correlation with steroid therapy) and increased numbers of cells of high peroxidase activity. Despite the common lymphocytopenia elevation of large unstained cells was noted in 20% of patients. There was no correlation between disease activity and any single laboratory measurement. However, no patient with severe disease had a lymphocyte count above 1.9 × 109/1 or a haemoglobin above 11.7 g/dl

ISSN:0001-5792    

DOI:10.1159/000207070

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

14 patients with chronic myelocytic leukemia were evaluated immunologically; no difference was found in mean lymphocyte percentage and absolute number between patients and healthy subjects. 4 cases (28.5%) showed decreased percentage of T lymphocytes, while only 2 cases (14.2%) had decreased absolute T lymphocyte values. PHA transformation was decreased in 57% of the patients, Spontaneous transformation in the short-term cultures exceeded the normal range in 65% of the cases. All patients skin tested were found to be reactive. Most of the patients had defective cellular immune response in vitro, probably related with a qualitative defect in T lymphocyte subpopula-tions. It cannot be completely excluded that part of the observed lymphocyte depression was due to the busulfan.

ISSN:0001-5792    

DOI:10.1159/000207071

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

107 determinations of α1-acid-glycoprotein (α1S), haptoglobin (Hp) and complement fraction 3 (C3) were made in 85 consecutive patients with Hodgkin’s disease, both in acute phase (77 measurements) and in complete remission (30 measurements). Only α1S and Hp showed much higher levels in untreated disease than in remission, and elevation of α1S in activity of the disease is correlated to advanced stages and – less significantly – to severe histology. The ability of α1S, Hp and C3 to discriminate between activity and remission was compared with that of erythrocyte sedimentation rate (ESR), α1S-globulinaemia (α2), fibrinogen-aemia (Fb), plasma copper (Cu) and iron (Fe), all data being collected at the same point in time in each patient. The well-known discrimination ability of combined Cu and Fe (75%) can be further improved by α1S (81%) much more than by Hp, C3, ESR, α2 and Fb singly computed and nearly up to the maximum allowed by the eight indexes

ISSN:0001-5792    

DOI:10.1159/000207072

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The immunochemical properties of high and low molecular forms of urokinase (HMW-UK, MW 53,000, 124,000 IU/mg protein; LMW-UK, MW 32,000, 230,000 IU/mg protein) were studied with specific antisera against the functionally active heavy chain (H chain, MW 31,000, 201,000 IU/mg protein) and the light chain (L chain, MW 18,000) of HMW-UK. Using a double-immunodiffusion technique, LMW-UK did not demonstrate L chain antigenicity in the molecule. Anti-L-chain serum exerted no effect on LMW-UK and the H chain, but anti-H-chain serum strongly inhibited the fibrinolytic activity of all the active enzymes (HMW-UK, LMW-UK, and H chain). Anti-L-chain serum was found to exert an antifibrinolytic effect on HMW-UK.

ISSN:0001-5792    

DOI:10.1159/000207073

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Peripheral blood monocytes isolated from patients with congenital hemolytic anemias, hereditary xerocytosis and spherocytosis, demonstrated in vivo engulfment of red cell and platelet fragments. In addition, morphometric studies performed on these monocytes showed an increase in cytoplasmic/nuclear ratio as well as lysosome and phagosome volumes. The production of carbon dioxide from glucose-1-14C in abnormal monocytes was increased (15–80%) but the intracellular values of beta-glucuronidase and esterase activity were similar to control monocytes. Monocyte locomotion assessed in the presence of chemotactic stimuli was found significantly increased (73 ± 12 monocytes/oil immersion fields vs. 46 ± 5 for control monocytes). We concluded that the monocytes in hemolytic anemias associated with increased in vitro red cell fragmentation have some features resembling the ‘stimulated’ monocytes and that this alteration may be due to red blood cell fragment in

ISSN:0001-5792    

DOI:10.1159/000207074

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A remarkable augmentation of Hb F and a reduction of Hb A2 were observed in a Sicilian woman during and after a course of treatment for Hodgkin’s disease. An inverse correlation between the proportion of Hb F and Hb A2 was found over an 8-year period, as well as in populations of red blood cells fractionated by density gradient. She exhibited two genetic defects, the Swiss type of heterocellular hereditary persistence of fetal hemoglobin and a β-thalassemia trait, which were confirmed by the study of the hemoglobin synthesis and by a family study. The lasting reactivation of Hb F synthesis is attributable to the interaction of several acquired and inherited facto

ISSN:0001-5792    

DOI:10.1159/000207075

出版商:S. Karger AG    

年代:1982

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000207076

出版商:S. Karger AG    

年代:1982

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000207077

出版商:S. Karger AG    

年代:1982

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000207078

出版商:S. Karger AG    

年代:1982

数据来源: Karger