摘要:

By a combined application of toluidine blue stain and naphthol AS-D chloroacetate esterase reaction on normal bone marrow smears metachromic and promyelocytic granules were demonstrated on the same cell. Precursors of basophils contain NASDCl-esterase activity, the intensity of which depended on the maturity of the cells. All transitional cells were found between non metachromatic promyelocytes with strong NASDCl-esterase activity and completely enzymatically negative metachromatic segmented basophils. It is concluded that basophilic granulocytes like monocytes, neutrophils, and eosinophils derive from non specific promyelocytes. Evidence has been presented for the transition of promyelocytic granules to specific basophilic granules. Our results confirm the dualistic view of the cytogenesis of leukocytes

ISSN:0001-5792    

DOI:10.1159/000208636

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

The cytoplasmic granules of the human basophil leukocyte have been shown to exhibit peroxidase activity both at the light and electron microscopic levels when exposed to the 3,3’- diaminobenzidine tetrahydrochloride peroxidase procedure. Reactivity is diminished when exogenous peroxide is omitted from the incubation medium and is completely abolished when catalase is included in the peroxide-free medi

ISSN:0001-5792    

DOI:10.1159/000208637

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

The transketolase activity (TKA) of red cells was determined in 443 subjects: 286 control subjects, 48 diabetics, 29 patients with hyperthyroidism, 8 with hypothyroidism, 29 chronic alcoholics, 25 patients with sideropenia, and 14 with dementia senilis. TKA levels significantly lower than those of the control series were recorded in chronic alcoholicsand patients with dementia senilis. The cause of this finding is discussed

ISSN:0001-5792    

DOI:10.1159/000208638

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

Heinz bodies were produced in vitroin normal human red cells by incubation with ascorbic acid, menadione, sodium azide, primaquine diphosphate, acetyl phenylhydrazine, potassium chlorate, sodium nitrite and p-phenetidine. All compounds causing Heinz body formation also produced methaemoglobin, and most led to reduction in catalase activity; sodium nitrite and p-phenetidine caused no inhibition of catalase. Catalase inhibition was not found with any of the drugs studied which did not produce Heinz bodies. Catalase inhibition appeared to be partly responsible for Heinz body formation by ascorbic acid and menadione, but not for the effects of the other drugs studied. The results of this study support the view that glutathione peroxidase is the most important factor in protecting haemoglobin from the action of oxidant drugs

ISSN:0001-5792    

DOI:10.1159/000208639

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

Erythropoietic cell proliferation of 6 cases of different clinical states of β--thalassaemia (thal) was studied by a combined method using Feulgen-cytophotometry and autoradiography after in vitro labelling with 3H-TdR. An abnormal distribution of the DNA content and 3H-TdR incorporation was found, which was more pronounced in the one patient with thal major than in the 3 patients with thal minor and was not provable in the subclinical form (minima), indicating a direct relationship to the ineffective erythropoiesis and the states of anaemia in this disease. This proliferation disturbance consisted of an accumulation of cells in G1, a decreased proportion of cells in S and a decreased S/G2 ratio, and was limited to the early polychromatic cell compartment. In the basophilic erythroblasts, a decreased proportion of cells in G1 was observed revealing a shortening of the G1 period in this cell compartmen

ISSN:0001-5792    

DOI:10.1159/000208640

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

The present study has been carried out to estimate the gene frequency of thalassemia and abnormal hemoglobins in a random Turkish population. A total of 1,000 Haemoglobin A2 blood samples have been studied. Fifteen of 900 adults had elevated Hb A2 concentrations. 0.60% of the population studied had an electrophoretically demonstrable hemoglobin abnormality: 3 Hb AS, 1 Hb AE, 1 Hb AD and one Hb A’2 (‘split’ A2). Signs of α-thalassemia were found in one case. This study indicates that the incidence of β -thalassemia trait in Turkish population

ISSN:0001-5792    

DOI:10.1159/000208641

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

Case report of a young woman, aged 27, suffering from severe megaloblastic anemia due to folic acid deficiency. The factor responsible for this deficiency seems to be the regular use of oral contraceptives for 3 years (Noracycline 22®). Recent investigation evidenced the existence of selective malabsorption of food folates, due to the action of hormonal contraceptives on the intestinal conjugase. It can thus be admitted that contraceptive drugs play a prominent part in the genesis of this type of anemia. However, other factors must also be considered, like malnutrition and a masked malabsorption syndrome. The relation with megaloblastic anemia in pregnancy is discusse

ISSN:0001-5792    

DOI:10.1159/000208642

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

A simple method of preparing stable suspensions of normal erythrocytes is described. These suspensions, stable for number, shape and volume, without aggregates and easy to use, are available as a standard of reference for the calibration and further controls of the electronic counters.

ISSN:0001-5792    

DOI:10.1159/000208643

出版商:S. Karger AG    

年代:1971

数据来源: Karger

摘要:

The in vitro proliferation and maturation of bone marrow cells in erythropoietic porphyria of cows was studied using 3H-thymidine labeling and autoradiography. Ratios of basophilic normoblast: polychromatic normoblast:late polychromatic normoblast: and orthochromatic normoblast were 1:3:8:19 for normal marrow and 1:3:12:32 for porphyric marrow. The turnover times for the basophilic normoblast: polychromatic normoblast: late polychromatic normoblast were 10:17:31 h in the normals as compared to 12:16:26 h in the porphyries. The cell cycle times and DNA synthetic times were correspondingly similar

ISSN:0001-5792    

DOI:10.1159/000208644

出版商:S. Karger AG    

年代:1971

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000208645

出版商:S. Karger AG    

年代:1971

数据来源: Karger