摘要:

A case of acute myeloid leukaemia (AML) (M2 type) is described in which the neutrophil population contained markedly increased peroxidase activity. The increased peroxidase activity was initially detected during routine counting with the Hemalog D automated differential counter and the increased enzyme staining was confirmed by manual and ultrastructural cytochemistry. The abnormal neutrophil population persisted after blast cells had disappeared from the blood and until marrow hypoplasia was induced by chemotherapy. When remission emerged, the peroxidase activity of the neutrophil population was normal. At subsequent relapse, the neutrophil population with increased peroxidase activity reappeared and with the induction of second remission neutrophil peroxidase again returned to normal. In 8 other cases where sufficient neutrophils were present for analysis by the Hemalog D, neutrophil peroxidase was normal or not increased in 7 cases, but was markedly reduced in 1 case of AML (M6 type). It is suggested that abnormalities of neutrophil peroxidase activity detected by the Hemalog D may serve as useful leukaemic markers.

ISSN:0001-5792    

DOI:10.1159/000206991

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The clinical pattern of haemolysis associated with Donath-Landsteiner antibodies has undergone a change over the years. In the current study 13 patients developed the acute form of the disease whilst only 1 presented with the classical picture of chronic paroxysmal cold haemoglobinuria. The acute illness typically occurred in young children with a male predominance of about 2.5:1; 10 patients were less than 5 years old. There.was often a history of a preceding infection, the onset was sudden, prostration, haemoglobinuria and pallor were prominent. The patients were very ill but rapid and complete recovery usually occurred within a few days; however, 1 patient died. Treatment consisted of rest and warmth; in addition blood transfusion was needed in 7 patients. The chronic disease (which was non-syphilitic in origin) followed a benign course, warmth and avoidance of cold being all that were necessary to maintain the patient’s well-being. It is felt that the general term for this disorder should be Donath-Landsteiner haemolysis rather than the traditional paroxysmal cold haemoglobinur

ISSN:0001-5792    

DOI:10.1159/000206992

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Treatment of human blood platelets with the thiol-oxidizing agent, diamide, causes rapid oxidation of glutathione and alterations in aggregation and release reaction. Moreover, cross-linking of proteins was observed. Three high molecular weight bands of 200 – 240 × 103 daltons and a band of 66 × 103 daltons were involved in this process. After reduction with dithiotreitol the normal pattern was received. In contrast to the erythrocyte, the cross-linking of platelet proteins was not accompanied by a reorientation of phosphatidylethanolamine in the membrane. Also a considerably smaller effect of diamide on platelet protein sulfhydryl group content was measu

ISSN:0001-5792    

DOI:10.1159/000206993

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The in vitro effect of diclofenac sodium (Voltaren) on the ultrastructure of healthy donors’ platelets was examined and compared with those induced by aspirin (ASA). In distinction to ASA, which causes loss of platelet pseudopodia, Voltaren induced an increase and marked elongation of these pseudopodia. The implication of this finding in the explanation of the decreased platelet aggregation caused by the drug is discussed. Voltaren increased the phagocytotic activity of the individual platelet, although the overall ability of the cells to phagocytize latex particles was not markedly increased. Platelets incubated with Voltaren showed a decrease in their total protein synthesizing capacity. Voltaren did not exert any effect on the internal ultrastructure, platelet factor 3, and calcium content of the incubated platelet

ISSN:0001-5792    

DOI:10.1159/000206994

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The serum of a patient with a clinically and immunologically identified multiple myeloma of the IgE class was found to contain both IgE-albumin and IgG-albumin complexes. These complexes were partially purified and some of their properties studied by biochemical and immunoehemical methods. The IgG-albumin interaction was dissociated by 5.0 M guanidine hydrochloride, while the IgE-albumin interaction was dissociated upon reduction by mercaptoethanol, suggesting that the proteins were linked by intermolecular disulfide bonds. Complex formation between pathological or normal immunoglobulins with albumin have been reported for IgG, IgM and IgA but not for IgD or IgE. The present observation seems to be the first in which an IgE myeloma protein was involved.

ISSN:0001-5792    

DOI:10.1159/000206995

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The unsaturated vitamin B12-binding capacity (UB12BC) and the three transcobalamins (TC I, TC II, TC III) have been studied in the serum of normal healthy Nigerian children. The serum UB12BC level was very high in the children: TC I accounted for 39.5% of UB12BC, TC II accounted for 49.5% and TC III accounted for 11%. The results are discussed in the light of the variations in the distribution of transcobalamins in children from what has been previously reported among the African adults. The high percentage of TC I in the children is discussed in the light of a possible immunological role of this trans-cobalamin. There was an inverse relationship between TC I and TC II levels. A sex difference in UB12BC and TC I levels was observed. The girls had higher UB12BC and this was due to higher TC I, but the advantage of this is not clear.

ISSN:0001-5792    

DOI:10.1159/000206996

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Angioimmunoblastic lymphadenopathy (AIL) is a severe disorder associated with immunological anomalies. The treatment of AIL is a much debated issue. This is the first report of a patient successfully treated by large doses of methylprednisolone given as intravenous pulse therapy after failure of chemotherapy.

ISSN:0001-5792    

DOI:10.1159/000206997

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The usual technique of harvesting bone marrow for allogeneic or autologous transplantation involves passage of the marrow suspension through discs of stainless steel mesh of increasingly small diameter. We describe a sterile technique which is much less messy and produces a single cell suspension. Potter-Elvehjem homogenizers are used to break up the marrow particles. This procedure has been used successfully in 6 patients where allogeneic transplantation was performed and in 6 harvests of autologous marrow. Marrow cryopreserved in this way contains viable committed stem cells and is not subject to agglutination following subsequent thawing.

ISSN:0001-5792    

DOI:10.1159/000206998

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Differential counts of smears and cytospins of full blood, dextran fractionated blood leukocytes and metrizoate/Ficoll fractionated mononuclear blood cells were compared. Frequencies of lymphocytes decreased in cytospins as compared with smears. The monocyte/ lymphocyte ratios were 0.24 in smears of full blood and 0.22, 0.31, 0.15 and 0.39 in smears and cytospins of dextran supernatant cells and metrizoate/Ficoll interface cells, respectively. Less than 5% of cells were broken in all preparations. The slide to slide variation was larger in preparations of fractionated cells than in smears of full blood. Cells were evenly distributed in mononuclear cytospins.

ISSN:0001-5792    

DOI:10.1159/000206999

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The plasma zinc and copper and urinary zinc levels were determined by an atomic absorption spectrophotometer technic in 14 patients with hemoglobin (Hb) H disease, 34 patients with β-thalassemia/Hb E disease and 30 control subjects. Plasma zinc levels were lower and the plasma copper levels were higher in the patients than in controls. The plasma Cu:Zn ratios, a more valuable indicator of body zinc status, increased in the diseased subjects, indicating zinc deficiency. Higher urinary zinc levels in the both Hb H disease and β-thalassemia/Hb E disease suggest that chronic hemolysis is responsible for hyperzin-curia leading to zinc deficiency. The thalassemic patients with undergrowth had higher plasma Cu:Zn ratios than the patients with normal development, indicating a more severe zinc deficiency in the forme

ISSN:0001-5792    

DOI:10.1159/000207000

出版商:S. Karger AG    

年代:1982

数据来源: Karger