摘要:

Pretreatment blood smears of adult patients with acute lymphoblastic leukemia were examined for neoplastic cells showing nuclear radial segmentation (RS). RS was present in 6 of 7 patients with immunologically proven T-cell leukemia, but not in 11 patients whose leukemic cells lacked T-cell markers. Electron microscopic studies of neoplastic cells showed multiple nuclear indentations and abundant cytoplasmic micro-filaments and microtubuli in connection with pericentriolar dense material. RS formation was decreased in 1 patient after chemotherapy with a vincristine-containing regimen, while the white cell count remained unchanged. Radial segmentation (RS) of leukocyte nuclei is a well-known phenomenon, which is observed ex vivo (Rieder cells), and can be induced in vitro. Convoluted or multilobulated nuclei in lymphoid neoplasms are similar to RS nuclei both with regard to their structure and their sensitivity to spindle-blocking drugs. We propose that the nuclear alterations observed in a variety of different T-cell neoplasms are identical with RS, and suggest that RS might be a morphological marker for subsets of lymphoproliferative disorders of T-cell origin.

ISSN:0001-5792    

DOI:10.1159/000206731

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Ultrastructures of normal T-cell subpopulations, Tγ and Tμ cells, were studied. Tγ cells were isolated and identified by repeating the rosetting method; firstly, by E rosette formation with neuraminidase-treated sheep red blood cells (SRBC), and next by EAy-rosette formation with ox red blood cells coated with IgG antibody (EAox). Before EAox rosetting, SRBC on isolated T cells were lysed by autologous plasma instead of ammonium chloride solution. Normal Tγ cells were heterogeneous with regard to their granules; the majority of Tγ cells had parallel tubular arrays (PTA) and a few had electron-dense granules. When ammonium chloride solution was employed to lyse SRBC, PTA were never observed; PTA in normal Tγ cells and in chronic lympho-cytic leukemia cells with Tγ character both seemed to change into electron-dense granules after ammonium chloride treatment. In contrast to Tγ cells, Tμ cells were characterized by clustered dense bodies, i.e. focal aggregates of electron-dense

ISSN:0001-5792    

DOI:10.1159/000206732

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Polycythaemia rubra vera (PRV) was diagnosed in a 69-year-old Nigerian woman whose haemolysate revealed an electrophoretically slow-moving homogeneous band of the enzyme glucose 6-phosphate dehydrogenase (G6PD). Further biochemical characterization identified the enzyme as a new sporadic G6PD variant. Electrophoresis of the haemolysate of 4 children of the proposita identified in a daughter a large quantity of the slow-moving variant enzyme, and a much lower quantity of the normal type enzyme, thus, signifying an extremely unbalanced mosaic phenotype situation. All other family subjects studied, including 2 sons, had the normal enzyme type B, thus confirming the heterozygosity of the proposita at the G6PD locus. Homogenates of platelets, granulocytes and E-rosette-forming lymphocytes of the proposita showed an identical slow-moving band as the haemolysate, thus, suggesting the origin of these cells from a common progenitor cell which may be either the pluripotent stem cell (PSC) or another precursor cell at an earlier level of development than the PSC. While the observation of preferential production of the variant enzyme may be attributed to the presence on the X chromosome of a ‘haemopoietic gene’, its relevance to the etiology of PRV is uncl

ISSN:0001-5792    

DOI:10.1159/000206733

出版商:S. Karger AG    

年代:1983

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000206734

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

An ultrastructural study of the aspirated bone marrow of a patient with congenital dyserythropoietic anemia type I is presented. Both in transmission electron microscopy (TEM) and in scanning electron microscopy (SEM) ultrastructural abnormalities of the bone marrow erythroid precursors were seen. These abnormalities in TEM included uneven condensation of chromatin with spongy appearance and alterations of cellular division. The most striking among these alterations were: firstly, the intercellular bridges which were typified by the presence of chromatin and the absence of the midbody and contractile ring and, secondly, the anomalies of the nuclear membrane. Occasionally, autolytic areas within the cytoplasm and iron-laden mitochondria were to be seen. In the SEM the following features were notable: the length of the intercellular bridges, the absence of the central ridge in the midbody area, as well as the scanty number of cytoplasmic blebs on the surface of the bridges.

ISSN:0001-5792    

DOI:10.1159/000206735

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

In vitro phagocytosis of platelets from patients with idiopathic thrombocytopenic purpura (ITP) (direct assay) and phagocytosis of normal platelets sensitized with patient serum (indirect assay), using normal peripheral blood leukocytes, were studied to find out whether the degree of phagocytosis reflects the severity of ITP. Phagocytosis was measured by the uptake of 51Cr-labeled platelets. Among patients whose platelet count was below 10 × lO4/μl, 92% showed an increase in the phagocytosis ratio by the direct assay and 67% by the indirect assay. There was a highly significant inverse correlation between the direct phagocytosis ratio and the platelet count (p < 0.001), but not between the indirect phagocytosis ratio and the platelet count. The amount of platelet-associated IgG (PAIgG) measured in patients whose platelets were used for the direct assay also showed an inverse correlation with the platelet count (p < 0.05). Vincristine administration provoked a reduction in of the direct phagocytosis ratio and PAIgG value. We propose that both the direct assay and PAIgG measurement reflect the severity of ITP, and PAIgG might be responsible for platelet phagocytosi

ISSN:0001-5792    

DOI:10.1159/000206736

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Fibrinogen Seattle, a clinically silent, slow-clotting dysfibrinogen, releases 50% of the normal amount of fibrinopeptide B as assessed by amino acid analysis. The reduced dysfibrin exhibited equal quantities of chains with Bβ- and β-charge mobility on polyacrylamide gel electrophoresis in 2 Murea at low pH. By these same techniques, the release of fibrinopeptide A was normal. Clots formed by repolymerizing the throm-bin and batroxobin dysfibrin monomers showed a maximal turbidity that was lower than normal. Fibrinogen Seattle was indistinguishable from normal Fibrinogen by radial immunodiffusion and immunoelectrophoresis. Degradation by plasmin and transamination by factor XlIIa were normal. The characteristics of Fibrinopeptide release by fibrinogen Seattle distinguish it from other reported dysfibrinogen

ISSN:0001-5792    

DOI:10.1159/000206737

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Chromosome studies were performed on peripheral blood (PB) cells with and without stimulation, and/or on bone marrow (BM) cells from 21 patients with chronic myeloid leukaemia (CML), and 18 patients with myelofibrosis (MF). Our results show that almost all the patients with immature granulocyte precursors in PB also had mitotic cells in their unstimulated PB. In CML all unstimulated mitoses had the Philadelphia chromosome. In each patient the abnormal karyotype in the PB was the same as in the BM. Because of the high frequency of dry taps in myelofibrosis, the tissue of choice for chromosome study is peripheral blood.

ISSN:0001-5792    

DOI:10.1159/000206738

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Folate binding in lysate prepared from leukocytes containing large amounts of the folate-binding protein was studied in equilibrium dialysis experiments (pH 7.4, 37 °C). Binding displayed positive cooperativity, and the binding affinity increased with decreasing concentration of binding protein (affinity constants in the range of 5 × 1091/mol to 7 × 1010l/mol). Both phenomena could be interpreted in terms of ligand binding to a polymerizing system where the affinity of ligand for the oligomer is greater than its affinity for the polymer prevailing at high concentrations of the binding protein. Binding affinity was decreased at a low temperature (7 °C) and particularly at pH 5.0 where positive cooperativity also disappeared. The binder (Mr∼25,000–30,000) eluted in the front effluent after DEAE-Sepharose® CL-6B chromatography of the lysate a

ISSN:0001-5792    

DOI:10.1159/000206739

出版商:S. Karger AG    

年代:1983

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000206740

出版商:S. Karger AG    

年代:1983

数据来源: Karger