摘要:

Cell kinetic changes induced in the marrow blasts by treatment with a triple cytotoxic regimen including daunorubicin, cytosine arabinoside and 6-thioguanine (DAT) were investigated in 6 previously untreated acute nonlymphocytic leukemia patients. A decrease in the labeling and mitotic indices was consistently observed 24 h after administration of daunorubicin, suggesting a G2 block and a preferential lytic effect on the S-phase cells operated by the drug. Conversely, cytosine arabinoside and 6-thioguanine in combination induced a series of kinetic perturbations variable from case to case; however, three principal patterns of kinetic response were recognized and discussed in detail. Useful information for the planning of a more rational antileukemic therapy can be drawn from a systematic study of the kinetic effects induced by drug combinations.

ISSN:0001-5792    

DOI:10.1159/000207666

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Mononuclear cells (MNC), isolated from peripheral blood of healthy donors, were cryoprotected by dimethyl sulfoxide and stored in liquid nitrogen. Colony-stimulating factor (CSF), produced by cryopreserved MNC, was compared with that of nonfrozen controls in a double-layer agar system with human bone marrow as target cells. Our results indicate that cryopreserved MNC retain their ability to stimulate myelopoiesis-committed stem cells after freezing. In addition, evidence was obtained that CSF of feeder layers changes, depending on the duration of preincubation and cell concentration. In a system where either stimulating or target cells are cryopreserved the dynamics of interactions between normal or abnormal cell lines can thus be studied.

ISSN:0001-5792    

DOI:10.1159/000207667

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The effects of an erythropoietin (Ep)-rich plasma fraction and of a commercial preparation of purified Ep (Step I) on the cyclic adenosine monophosphate (cAMP) levels of suspended rabbit bone marrow cells at different incubation times (up to 10 min) were investigated. Both Ep preparations caused an early increase in cAMP concentration: using the plasma fraction a significant increase was found at the 6th, 7th, 9th and 10th min of incubation, whereas the purified Ep produces a significant increase at all incubation times, starting from 1 min. No effect was observed following incubation with fluorescein isothiocyanate (FΙTC)-inactivated Ep. These findings are discussed and a possible role of cAMP as the intracellular messenger for Ep action is suggested

ISSN:0001-5792    

DOI:10.1159/000207668

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The erythroid repopulating ability (ERA) of femoral bone marrow cells was determined in polycythaemic CBA, C57B1 and Balb/c mice. Polycythaemia was induced by hypertransfusion or exposure to chronic hypoxia in silicone rubber membrane enclosures. The results obtained demonstrate decreased ERA after both types of polycythaemia, with the exception of ex-hypoxic Balb/c mice. A decrease in the number of erythroid repopulating cells in DNA synthesis was found in hypertransfused and exhypoxic CBA mice in comparison to normal values. These studies indirectly indicate the contribution of cells committed to erythropoiesis in measurement of ERA.

ISSN:0001-5792    

DOI:10.1159/000207669

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

2 cases of acute myeloid leukaemia with inclusion bodies are presented. The inclusions were found mainly in the blast cells but could also be encountered in lymphocytes and plasma cells. Cytochemical and ultrastructural studies showed a great resemblance of these inclusions to the ones found in Chediak-Higashi anomaly, i.e., high acid phosphatase activity, varying in size of inclusions from clusters of small granules to huge inclusion, sometimes found in vacuoles, featuring fusion of lysosomes.

ISSN:0001-5792    

DOI:10.1159/000207670

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Hematological data, biosynthetic studies and γ-chain structure of three heterozygotes for HPFH Greek type and of two heterozygotes for both HPFH and β-thalassemia are reported. In the HPFH heterozygotes, hematological data were normal and globin chain synthesis balanced, while subjects carrying both HPFH and β-thalassemia presented a thalassemic picture and the same degree of α/non-α-chain imbalance as the β-thalassemia carrier belonging to the same family. The γ-chain composition studies showed only the presence of Aγ-chains in HPFH; in the association HPFH/β-thalassemia also some Gγ and Τγ were found. The mechanisms determining the high production of Hb F in the association HPFH/β-thalassemia a

ISSN:0001-5792    

DOI:10.1159/000207671

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

A fifth family with the unstable Hb Genova is reported from Kenya, East Africa. This almost certainly represents an independent mutation. The beneficial effect of splenectomy has been carefully assessed in one member of the family.

ISSN:0001-5792    

DOI:10.1159/000207672

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Reticuloendothelial phagocytic capacity (REPC) was determined in 14 children with β-thalassemia major, by means of technetium 99m sulfur colloid uptake, who had not had splenectomy. No difference was observed in the REPC between patient and controls. The REPC of liver and spleen were evaluated separately by determining the half-time for the clearance of 99mTc from the blood. The REPC of both liver and spleen in patients was expected to be decreased when compared with controls; however, we found that the REPC of the liver was increased in patients and that there was no difference between patients’ and controls’ spleen values. This suggests that chronic anemia and hemosiderosis do not alter the REPC in β-thalassemia

ISSN:0001-5792    

DOI:10.1159/000207673

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Erythrocyte PK deficiency was detected in a family from Latium in Italy. This PK variant is characterized by normal or increased activity immediately after blood collection, instability to storage, to heat and to urea. Only in the propositus the mutant enzyme exhibited an increased Michaelis constant for PEP, slightly increased inhibition by ATP and an altered optimum pH value. The kinetic anomaly was only partially corrected by activation with F-1, 6-DP and by addition of 2-ME. From these results it can be concluded that in the family observed two distinct erythrocyte PK alterations were demonstrable: instability in the propositus and his father; low affinity for PEP and altered optimum pH value only in the propositus.

ISSN:0001-5792    

DOI:10.1159/000207674

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The unstable hemoglobins Istanbul and Saint-Etienne have the same amino acid substitution (α2β2 92F8 His→Gln). Despite this, there are some clinical and hematological differences between the individual with Hb Istanbul and the one with Hb Saint-Etienne. These are: (1) the clinical course of the patient with Hb Istanbul before splenectomy was more severe when compared with that of the individual with Hb Saint-Etienne; (2) although Hb F was found within normal range in the individual with Hb Istanbul, a high level of Hb F (19%) was observed in the patient with Hb Saint-Etienne. The alkali-resistant Hb found in the former was of adult type and in the latter case it was of fetal type. The possible explanations for these differences are discus

ISSN:0001-5792    

DOI:10.1159/000207675

出版商:S. Karger AG    

年代:1979

数据来源: Karger