摘要:

The polymerase chain reaction (PCR) and hot-blotting methods were used to identify fibroblast growth factor (FGF) receptor- 1-specific messenger ribonucleic acid (mRNA) sequences in cDNA samples prepared from human corneal endothelial cell cultures with proliferative and senescent morphology, an ex vivo corneal epithelium sample, two primary corneal epithelial cell cultures, two third-passage corneal epithelial cell cultures, and two stromal fibroblast cultures. The PCR primers used in this study distinguished mRNAs coding for three aminoterminal motifs (alpha, beta, and gamma) of the FGF receptor- 1 that are derived by alternative splicing from a single genomic sequence. Messenger RNA molecules coding for FGF receptor-1 amino-terminal motif were detected in corneal endothelial and epithelial cells. The alpha and beta amino-terminal motif, but not the gamma amino-terminal motif, mRNAs were detected in stromal fibroblasts. The gamma motif lacks a known signal sequence for membrane translocation and is thought to represent an intracellular form of the FGF receptor-1. Identification of mRNA coding for FGF receptor-1 along with the previous identification of basic FGF mRNA and protein in corneal endothelial, epithelial, and stromal fibroblast cells suggests an autocrine and/or paracrine role for basic FGF in the physiology of the cornea.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

Corneal histopathology is essential to understanding the mechanisms of corneal disease and wound healing. We have developed a light microscopic trichrome stain for 1-µm, aldehyde-fixed, osmicated, epon/resin-embedded corneal sections. The stain consists of azure II, methylene blue, and basic fuchsin. This combination of stains provides a fast and effective method to clearly differentiate corneal cells from their surrounding extracellular matrix, and chromatically separates various ECM materials within the cornea. Correlative examination by light and transmission electron microscopy (TEM) provides multilevel understanding of corneal morphology and ultrastructure. Tissues examined using this staining procedure may be subsequently examined by TEM for ultrastructural morphology. This stain has proved beneficial in our research of both cellular and extracellular constituents of the cornea.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

There recently have been several reports of microsporidial keratoconjunctivitis caused by the organism Encephalitozoon hellem. However, treatment of this infection has been largely ineffective. We report a case of a 35- year-old Hispanic woman with AIDS and E. hellem keratoconjunctivitis confirmed with light, electron, and immunofluorescence microscopy that resolved promptly with topical fumagillin, a crystalline antibiotic with proven efficacy against Encephalitozoon species. No corneal or systemic toxicities were noted using the dosage and preparation employed (10 mg/ml suspension in balanced salt solution). An easily prepared, topical fumagillin suspension appears to be a safe, effective treatment for E. hellem keratoconjunctivitis.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

A 29-year-old female intravenous drug abuser infected with the human immunodeficiency virus suffered recurrent, bilateral corneal infections over an 11-month period. Multiple infectious organisms were responsible, including capnocytophaga species, Candida albicans, Staphylococcus aureus, coagulase-negative staphylococcus, and a-streptococcus. One eye was eviscerated because of corneal perforation and loss of vision; the second eye has maintained good vision. Predisposing factors usually associated with corneal infections were absent. Treatment was complicated by extremely poor patient compliance, ongoing intravenous drug abuse, and concurrent multiple extraocular medical problems.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

The cornea remains clear and avascular partly as a result of the compactness of the stromal lamellae. Loosening of these lamellae promotes loss of corneal transparency and peripheral vascularisation. Conversely, increased compactness of corneal tissue may possibly result in resorption of certain peripheral opacities. We report a case in which increased corneal compactness, produced by dellen formation, resulted in the disappearance of a type II Vogt's limbal girdle.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

Two patients developed infectious crystalline keratopathy (ICK) 9 and 11 months after penetrating keratoplasty. In both cases, cultures yielded enterococcus sensitive to vancomycin. The first patient developed diffuse loss of corneal and conjunctival epithelia after 24 h of treatment with vancomycin eyedrops, whereas the second patient did not respond after 14 days of topical vancomycin therapy. Both patients eventually required penetrating keratoplasty. Enterococcus is a ubiquitous bacterium resistant to many antibiotics that should be suspected of causing ICK.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID