摘要:

Invasion of polymorphonuclear neutrophils (PMN) into injured cornea is one of the early events in corneal wound-healing. In the present studies, we examine the mutual effects on protein synthesis by PMN and injured and normal corneas when they are cocultured. PMN were labeled with [35S]methionine in the presence or absence of normal or alkali-injured rabbit corneas for 1-5 h. The acid-insoluble35S-labeled proteins in medium, cells, and tissues were measured. Our data indicate that alkaliinjured rabbit corneas induce higher rates of incorporation of [35S]methionine and secretion of35S-labeled newly synthesized proteins by PMN. The newly synthesized35S-labeled proteins were then analyzed by twodimensional PAGE. The results indicate that alkaliinjured and normal rabbit corneas enhance the synthesis and secretion of a 18-kD protein by PMN. In contrast, alkali injury greatly reduced the secretion of a group of proteins having molecular weights of ~30 kD by rabbit corneas. The alkali-injured corneas synthesize a new 200- kD protein (AC-200) in tissues and increase the secretion of a 120-kD protein (AC-120) into the culture medium. Furthermore, PMN slightly inhibits the incorporation of [35S]methionine and alter the synthesis of several35S-labeled proteins by normal and injured corneas. For example, incubation with PMN abolishes the synthesis of the AC-200 protein, but enhances the secretion of the AC-120 protein by the alkali-injured corneas. However, the function and nature of these proteins remain largely unknown. Further studies are needed to elucidate the biological roles of these polypeptides during corneal wound healing.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

We describe a 50-year-old man with a 10-year history of gradually enlarging limbal tumors and conjunctival injection. An excised limbal mass presented a diagnostic challenge histopathologically and was initially believed to be a fibrous histiocytoma. Special stains, however, revealed acid-fast organisms in the fibrous histiocytes, and the diagnosis was changed to corneal leproma. This case confirms the existence of fibrous histiocytoma-like lesions in leprosy, which in this form can be termed fibrous histiocytoid leprosy. Furthermore, it suggests the need for special stains to rule out infectious cause in lesions believed to be atypical fibrous histiocytomas.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

A 73-year-old man had only light perception vision from a slowly enlarging fleshy papillomatous mass covering the entire cornea. The lesion was excised completely, leaving a clear corneal bed and 20/25 vision. Histopathologically, the lesion was found to be squamous cell carcinoma in situ with early microinvasion of the basement membrane. Viral studies for human papilloma virus were negative. This case represents a massive superficial involvement of the cornea by squamous cell carcinoma and shows the resistance of Bowman's membrane to tumor penetration.

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID

ISSN:0277-3740    

出版商:OVID    

年代:1993

数据来源: OVID