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11. |
Transplantation of Corneal Endothelial Cells Using a Cell Carrier Device |
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Cornea,
Volume 13,
Issue 2,
1994,
Page 173-182
Judit Mohay,
Thomas Lange,
Joern Soltau,
Thomas Wood,
Barbara McLaughlin,
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摘要:
Penetrating keratoplasty is currently the only treatment for corneal endothelial dysfunction. Although corneal transplantation has a high success rate, a few problems still remain, such as the limited availability of donor grafts, the change in refraction after penetrating keratoplasty, and the higher chance of immune rejection. In this study, a coated hydrogel lens (Chiron Ophthalmics Inc., Irvine CA, U.S.A.) has been used as a carrier to transplant cultured homologous kitten and rabbit corneal endothelial cells into adult cats and rabbits. The transplantation procedure was the same in both species. Corneal endothelial cells from homologous rabbits or cats were seeded on coated hydrogel lenses and cultured until they reached a complete monolayer with an average cell density of 2,500 cells/mm2. Five weeks before transplantation surgery, corneal endothelial cells were scraped to induce corneal edema. The cell carrier device was then transplanted as follows: a trephine cut (7.7 mm) was made into the stroma, producing an outer corneal plug. The inner cornea was then cut by using a 5.5-mm trephine, and this inner plug was discarded. The implant was inserted and the outer corneal plug was sutured back into place. Corneas cleared completely within 3 days in both rabbits and cats, and stayed clear for an average of 40 days in rabbits and 50 days in cats. The histopathological evaluation of the rejected grafts showed vascularized retrocorneal membrane formation in cats, whereas in rabbits severe cellular infiltration of the stroma with neovascularization occurred without retrocorneal membrane formation.
ISSN:0277-3740
出版商:OVID
年代:1994
数据来源: OVID
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12. |
In Vivo Scanning Slit Confocal Microscopy of Acanthamoeba KeratitisA Case Report |
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Cornea,
Volume 13,
Issue 2,
1994,
Page 183-185
James Auran,
Michael Starr,
Charles Koester,
Vincent LaBombardi,
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摘要:
A 29-year-old woman presented with clinical signs and symptoms of Acanthamoeba keratitis. Scanning slit confocal microscopy revealed a 26-p.-diameter object, resembling an Acanthamoeba cyst, in the anterior stroma. Numerous ovoid objects (possibly inflammatory cells, trophozoites, or altered keratocytes) were present. Normal keratocyte nuclei and the anterior corneal mosaic, readily imaged by scanning slit confocal microscopy of the normal cornea, were noticeably absent. Subsequent corneal biopsy confirmed the diagnosis of Acanthamoeba keratitis.
ISSN:0277-3740
出版商:OVID
年代:1994
数据来源: OVID
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13. |
Acanthamoeba KeratitisResolution After Epithelial Debridement |
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Cornea,
Volume 13,
Issue 2,
1994,
Page 186-189
James Brooks,
Douglas Coster,
Paul Badenoch,
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摘要:
Two patients presented with dendritiform epithelial irregularities of the cornea that were unresponsive to topical acyclovir. Both were soft contact lens wearers who had practiced inadequate lens hygiene. Acanthamoeba was cultured from a shallow corneal biopsy of one patient and from the contact lens of the other. Epithelial debridement resolved the condition in both patients without concomitant treatment with antiamoebic agents.
ISSN:0277-3740
出版商:OVID
年代:1994
数据来源: OVID
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14. |
Primary Gelatinous Drop-Like Corneal Dystrophy in a White WomanA Pathologic, Ultrastructural, and Immunohistochemical Study |
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Cornea,
Volume 13,
Issue 2,
1994,
Page 190-194
Ernst Btichi,
Basil Daicker,
Sylvie Uffer,
Fred Gudat,
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摘要:
A white female with primary gelatinous drop-like corneal dystrophy (PGDD) was followed from the ages of 8 to 37 years. During this time, she underwent repeated lamellar and penetrating keratoplasties on both sides due to relapsing bilateral corneal opacifications. The diagnosis of PGDD was based on the histology of the corneal specimens obtained at 10 and 36 years of age, as well as on the characteristic clinical appearance and course. Electron microscopy showed typical amyloid fibrils. Immunohistochemical staining was mildly positive for amyloid AL (light chain), but negative for amyloid AA, AF, AB, and keratin. Thus, the precursor protein of the amyloid deposits in PGDD might derive from immunoglobulins, e.g., from the tear film. However, the precursor protein might derive from a different origin altogether. In that case, the positive staining for amyloid AL would translate nonspecific absorption of fragments of light chains.
ISSN:0277-3740
出版商:OVID
年代:1994
数据来源: OVID
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15. |
Eye Bank Association of America: Research Awards |
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Cornea,
Volume 13,
Issue 2,
1994,
Page 195-195
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PDF (24KB)
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ISSN:0277-3740
出版商:OVID
年代:1994
数据来源: OVID
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