摘要:

SummaryWe have assessed the role of tumour necrosis factor‐α (TNF) during cutaneous leishmaniasis and demonstrated that significant levels of TNF were released by spleen cells from infected mice afterin vitrorestimulation withLeishmania majorpromastigotes. Spleen cells from both genetically resistant and genetically susceptible mice were equally capable of producing TNF. After challenge with bacterial endotoxin, TNF activity could also be demonstrated in the serum ofL. major‐infected mice and the titres correlated with the course of cutaneous disease in susceptible and resistant mice. TNF did not exert a direct leishmanicidal effectin vitro.Furthermore, our study indicated that macrophages are the source ofL. major‐induced TNF activity and that its elicitation is dependent on the presence of T cells. These findings suggest that TNF acts in concert with other cytokines produced duringL. majorinfection and that its role depends on the composition of T cell subsets and cytokines p

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1990.tb00983.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryIn order to characterize the antigenic determinants ofLeishmania aethiopica, we have analysed by immunoblotting the antibody reactivity of leishmaniasis patients with either the localized (LCL) or diffuse (DCL) clinical forms of disease. In this study we have compared the reactivity of antibodies from eight LCL and DCL patients to parasites isolated from each individual, or the parasite isolates of the other LCL and DCL patients studied. The immunoblot profiles of antibodies from LCL patients differed from the antibody profiles of DCL patients. Serum antibodies from LCL patients showed limited recognition of somatic antigens of

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1990.tb00984.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryTwo panels of H‐2 recombinant mice were used in a detailed serological study to analyse the role of H‐2‐linked genes in the control of the antibody response to excretory/secretory (E/S) antigens ofTrichuris muris.An apparentH‐2q(1‐Aq) restriction on the early development of high levels of IgGl antibody to E/S antigen was revealed by ELISA. No such restriction was demonstrated for the specific IgG2a response patterns. Recognition of two high molecular weight antigens (90–95 kDa, 105–110 kDa) by IgG antibodies was also shown to be almost exclusivelyH‐2qrestricted and may be related at least in part to the high antibody levels seen forH‐2qstrains of mice. Immune serum from resistant (B10.BR × B10.G) Fl hybrid mice (H‐2qk) containing high levels of IgGl antibodies specific forT. murisE/S and IgG antibodies which recognized the 90–95 kDa and 105–110 kDa E/S antigens was effective in transferring protection to the non‐responsive B10.BR mouse strain as seen on day 35 post‐infection (p.i.). It is suggested that the IgG responses described for the generally very resistantH‐2qmouse strains may contribute to, but not be an absolute requirement for. protective immunity, antibody‐mediated damage facilitating a subsequent cellular a

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1990.tb00985.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryDuring infection withSchistosoma mansonithe extent and nature of immune reactions against schistosomula may be influenced by responses to cross‐reactive antigens in eggs or adult worms, and there is now extensive evidence for cross‐reactivity between the different stages of the parasite life cycle. In this study IgM and IgG subclass antibodies produced in (CBA/N × Balb/c) Fl male and female mice were measured over a period of time following exposure to a chronic infection, to unisexual male cercariae or to irradiated larvae. Antibody levels were also measured following immunization with antigen preparations derived from adult worms, schistosomula or eggs. (CBA/N × Balb/c) Fl male mice exhibit an X‐linked immune deficiency which results in an inability to respond to T‐independent (TI) type 2 polysaccharides. Isotype levels were measured by ELISA to detergent‐soluble schistosomulum antigen. Results showed that antigens on the different stages of the parasite life cycle have a qualitative influence on the antibody response to the larval surface, and that T‐independent type 2 polysaccharides, particularly abundant in egg. exhibit antigen‐directed isotype restriction in the form of IgM and

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1990.tb00986.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryMonoclonal antibodies (MoAb) were produced against somatic antigens of adult human liver flukeOpisthorchis viverrini.Earlier studies attached diagnostic potential to an 89–90 kD antigen present in both somatic extracts andin vitroculture supernatants as well as to the abundant 16–17 kD tegumental protein doublet. Mice made excellent immune responses to low dose somatic extract adsorbed onto nitrocellulose or to the 80–95 kD region of SDS gel Western blots. The antigen specificities of hybridomas reactive with somatic antigen by ELISA were determined by radioimmunoprecipitation or immunoblotting. Six MoAb reacted with the desired 16 kD tegumental protein. A 90 kD somatic protein was identified by 9 clones. By indirect immunofluorescence, monoclonals reactive with the 16 kD polypeptide identified the outermost surface of the tegument. The 90 kD antigen was associated with all major muscle systems, most strikingly the crossed subtegumental layers, oral and ventral suckers, pharynx and a thin layer surrounding caeca. The biochemical identity of this muscle‐associated antigen is unknown, but it is clearly distinct from the previously identified species‐specific 89 kD exoantigen. The 16 kD tegumental protein shares epitopes with a number of related flukes. However, 2 MoAb which react with this protein show no cros

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1990.tb00987.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY