摘要:

Previous studies have demonstrated that shistosomula, recovered from the lungs ofSchistosoma mansoni‐infected mice, acquire H‐2Kkand both Ikand Isgene products. We confirmed and extended those observations by identifying several individual antigenic specificities mapping not only to H‐2Kkand I‐Ak(Sher, Hall&Vadas 1978), but to H‐2Dk, I‐Ek, H‐2Kb, H‐2Dband I‐Abas well. Private H‐2 specificites 23 (H‐2Kk) and 32 (H‐2Dk) were identified on the tegumental surface of schistosomula isolated from C3H/Crgl mice (H‐2k). Larvae isolated from C57Bl/10Sn (B10) mice (H‐2b) were shown to acquire private specificities 33 and 2, which map to H‐2Kband H‐2Dbrespectively. I region associated (Ia) antigens coded for by genes mapping in the I‐A subregion (Ia. 2 and 3) and I‐E subregion (Ia. 7) were also acquired from C3H/Crgl mice. Schistosomula from B10 donors were shown to acquire Ia specificities 3 and 8, which are under I‐A subregion control. Evidence that monoclonal antibodies recognize H‐2 antigens on

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00450.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The level and specificity of antibody responses elicited in cattle by irradiated non‐infectiveTrypanosoma bruceiwere examined and related to the development of protective immunity. These responses were compared with those induced by infection and by inoculation with purified variable surface glycoprotein (VSG) in adjuvant. It was found that 107or more irradiated trypanosomes inoculated intravenously into cattle conferred complete protection against challenge with 103homologous trypanosomes 14 days later. Animals immunized with 106organisms showed partial protection. Of the assays used for detection of antibody, neutralization of infectivity was slightly more sensitive than either the Farr assay or the immunofluorescence test which were both more sensitive than solid‐phase radioimmunoassay (RIA). Detection of specific antibody correlated with immunity, in that all animals inoculated intravenously with 106or more trypanosomes developed neutralizing activity in their sera. The antibody responses after intravenous inoculation were consistently superior to those induced by the subcutaneous route. By carrying out blocking assays, most of the antibody elicited by irradiated trypanosomes was found to be specific for antigenic determinants on the VSG exposed on the surface of live trypanosomes. A similar specificity was found for the antibody induced during infection withT. brucei.Conversely, a large component of the antibody induced by purified VSG in adjuvant appeared to be directed against determinants on the VSG which are not exposed on live trypanosomes and are probably not involved in protective immun

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00451.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Antigenic determinants present on the surface of squirrel monkey erythrocytes infected with late developmental stages ofP. falciparumwere recognized through their ability to bind antibodies present in the serum of immune monkeys. Two different assays were used to demonstrate the presence of these antigenic determinants on the cell surface: the fluorescent labelling of intact cells in suspensions and the binding to protein A‐Sepharose. Such determinants appear to be strain‐specific and have, for a given strain, a variable expression according to whether the parasites are taken from intact or splenectomized animals or whether they have been grownin vi

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00452.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Serum and ascitic fluid from squirrel monkeys (Saimiri sciureus) inoculated with erythrocytic stages ofPlasmodium falciparumwere collected at different periods of the infection. Protection againstP. falciparumwas achieved by passive transfer of the sera or fluid recovered from animals after spontaneous or drug‐induced cure. Purified immunoglobulins from the ascitic fluid also conferred protection. In contrast, protective antibodies directed against erythrocytic stages ofP. falciparumcould never be demonstrated during the acute phase of infection in spite of the high titres of malarial antibodies detected by immunofluorescence. The comparative immunochemical analysis of antigens recognized by protective and non‐protective antibodies revealed quantitative differences which may be of use for the identification of antigens inducing protect

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00453.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Infections ofSchistosoma japonicumwere studied in mice which were immunosuppressed either by thymectomy and administration of antithymocyte serum or by treatment with hydrocortisone acetate. The relation ofS. japonicumwith the immunosuppressed host differed with that reported forSchistosoma mansoni.The pathogenesis of theS. japonicuminfection in the immunosuppressed host was less severe than that caused byS. mansoni, with respect to survival of, and hepatocellular damage to, the host. In contrast withS. mansoni, S. japonicumdid not have a reduced fecundity in immunosuppressed mice and there was no significant reduction in the numbers of faecal eggs excreted by these hosts.

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00454.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Infection of mice withTrypanosoma cruzihas been shown to lead to an impaired ability of lymphocytes to proliferate in response to mitogenic stimulation which is manifested during the acute period of the disease. A possible involvement of suppressor T lymphocytes has been postulated by other authors and was investigated in this work as a part of our efforts to disclose the mechanisms underlying the immunologic deficiency. Spleen cells from acutely infected CBA/J mice readily exhibited unresponsiveness to stimulation with concanavalin A, phytohaemagglutinin or a bacterial lipopolysaccharide. However, these cells were unable to reduce the responses that normal syngeneic‐mouse spleen cells mounted to these mitogens when cultured together in equal proportions. Furthermore, removal of the Lyt 2.1‐bearing cells, known to include the suppressor T cell subpopulation, from infected mouse splenocyte suspensions, did not alter the deficient responsive status of the remaining cells. These results, together with the severe depletion of the T‐cell compartment which occurs in the spleens of animals acutely infected withT. cruzi, do not support an important role of suppressor T lymphocytes in the noted deficiency in lymphoid cell reactivity to mitogens. Reduced numbers of responder cells, intrinsic lymphocyte alterations or suppression by cells other than T lymphocytes remain plausible explanations to be exp

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1982.tb00455.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY