摘要:

SummaryThe p190 protein (also called MSA1 or MSP1) of the asexual blood stage forms of Plasmodium falciparum, a human malaria vaccine candidate, shows polymorphism between different isolates. Mice were immunized with p190–3, a recombinant protein which contains mostly conserved sequences derived from the p190 protein of the K1 parasite isolate. Proliferative T‐cell responses of lymph node cells from immunized mice were assessed by stimulation in vitro with p190–3 or preparations of parasitized red blood cells (PRBC) containing the native protein. The p190–3‐specific T cells from C57BL/6 mice consistently responded to some P. falciparum isolates, representing either the K1 or MAD20 serotype of p190, but not to other P. falciparum strains or to rodent malaria parasite‐infected red blood cells. p190–3‐specific T‐cell responses from other mouse strains (BALB/c, C3H/He) did not distinguish between P. falciparum isolates. The polymorphic epitopes which were preferentially recognized by T cells from C57BL/6 mic

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00592.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryPrevious results, using a passive transfer assay, have shown that recrudescences of Plasmodium chabaudi chabaudi AS strain are antigenically different from the infecting parental population and also that the recrudescence appears to be a mix of antigenic types. This present study examines further these recrudescent populations using an indirect fluorescent antibody test on live, schizont‐infected red blood cells. This analysis shows that ten clones derived from a recrudescence are all antigenically different from the parent population and that some are different from each other. The use of this method to examine the antigenic types of recrudescent clones after transmission through mosquitoes also demonstrates a resulting change in antigenicity. Such results showing a link between mosquito transmission and varying antigenicity may have important implications in terms of immunity and vaccine developmen

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00593.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryThe transfer of spleen cells from (BALB/cXC57Bl/6) F1 mice recovered from a Plasmodium chabaudi chabaudi AS infection into irradiated syngeneic recipients conferred protection. Neither elimination of Thy‐1+cells nor in vitro irradiation of immune cells before transfer affected protection while both anti‐Thy‐1 treatment and irradiation abolished the appearance of anti‐P. c. chabaudi antibodies in the recipients. Superinfection of immune spleen cell donors did not improve their capability to transfer protection which was also unaffected by anti‐Thy‐1 treatment. The serum of mice after one infection was only marginally protective when transferred into irradiated recipients and a second infection improved the protective activity of serum which was not further improved by six infections. The cotransfer of immune serum and immune cells did not result in any synergistic effect. On the other hand, when P. c. chabaudi AS (BALB/cXC57B1/6)F1 infected mice were challenged with a high dose of Plasmodium yoelii 17XL at crisis, the mice were unable to control the heterologous parasite. When mice were challenged with P. yoelii 17XL several weeks after infection with P.c. chabaudi AS, a good degree of cross‐protection

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00594.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryBALB/c mice immunized with L3 of Brugia pahangi, irradiated at 45kRad from a137Caesium source, are strongly immune to challenge infection (75–100% reduction in the recovery of challenge infection larvae on day 6 post‐challenge). The target of immunity appears to be the post‐infective L3, as challenge infection larvae are killed within 5–6 days of infection. By immunoblot analysis, serum from immune animals recognizes a limited set of somatic antigens, the majority of which are shared between different life cycle stages. Serum from immune mice also strongly recognizes larval surface antigens by immunofluorescence, some of which may be stage specific. The larval surface determinants do not appear to be protein or glycoprotein by standard immunochemical analysis. A proportion of the antibody response of the BALB/c mouse is directed towards phosphorylcholine epitopes on filarial antigens, but the limited antigen recognition cannot be explained on the basis of the mouse strain used, as CBA/Ca mice recognize a similar limited set of a

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00595.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryIn vitro released products of the adult stage of the bovine lungworm, Dictyocaulus viviparus, were characterized according to their SDS‐PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with35S‐methionine indicated active synthesis of ES throughout this time. There was, however, little incorporation of3H‐glucosamine into ES products, and lectin affinity chromatography and glycopeptidase F digestion identified only one glycosylated component. Immunoprecipitation of125l‐labelled ES products with sera from calves patently infected with D. viviparus demonstrated that all of these, with the exception of two components, are antigenic to the bovine host. One of those not immunoprecipitated was shown to be host serum albumin carried over into culture. A limited degree of cross‐reactivity between nematode species was observed, with a D. viviparus female‐specific antigen of 290 kDa being recognized by serum antibody from calves infected with the gastrointestinal nematodes Cooperia oncophora and Ostertagia ostertagi. Calves vaccinated with irradiated larvae of D. viviparus, despite not being exposed to the adult stage of the parasite, also showed some recognition of adult ES products. This might suggest that vaccination with irradiated larvae operates against both pre‐pulmonary and pulmonary stages of

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00596.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryThe kinetics of daily faecal egg count, worm burdens, and intestinal cellular responses were examined in Mongolian gerbils after infection with either Strongyloides venezuelensis or Nippostrongylus brasiliensis alone, or concurrently with both parasites. The results show that, both in individual and concurrent infections, S. venezuelensis infection persisted for over 10 weeks and elicited a gradual increase in number of mast cells in the jejunal mucosa. On the other hand, N. brasiliensis worms were expelled by 3 weeks in association with goblet cell hyperplasia. These results suggest that effector/regulator cells involved in worm expulsion are different and highly selective depending on the genus of intestinal helminths.

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00597.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryWe report the use of a matched set of mice/human chimaeric antibodies, directed against the 5‐iodo‐4‐hydroxyl‐3‐nitrophenacetyl (NIP) hapten, to investigate the roles of different human isotypes in antibody‐mediated eosinophil‐dependent killing of schistosomula. The chimaeric antibodies consist of mouse VH, VL and CL regions with human γ1, γ2, γ3 (2 allotypes), y4, α2, or e CH regions and were used in in vitro assays with human eosinophils and NIP‐coated S. mansoni schistosomula. Some anti‐NIP isotypes mediated high levels of killing, which was specific for NIP‐coated larvae, and we suggest that these antibodies will be a valuable tool for studies on the role of antibody isotypes in anti‐schistosome immune effector mechanisms. In particular, this method directly demonstrated, for the first time, that IgA is highly effective in mediating the killing of metazoan paras

ISSN:0141-9838    

DOI:10.1111/j.1365-3024.1993.tb00598.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY