摘要:

In the present paper we show that in dogs, naturally infected withLeishmania infantum, the aqueous humour and the cerebrospinal fluid contain anti‐LeishmaniaIgGs and that the specificity of antigen recognition of these fluids is similar to that of the sera. We also show that in the encephalon and cerebellum of these dogs there is a pathological sponge‐like reaction accompanied by neuronal degeneration, mobilization of glial cells together with accumulation of amyloid deposits. The interstitial and intravascular deposition of IgGs andLeishmaniaantigens in choroid plexus suggest that in these animals there is a failure of the blood‐cerebrospinal and ciliary bodies filtration barriers which may allow the transfer of anti‐LeishmaniaIgGs from the blood stream to these fluids. We suggest that the failure of the blood‐cerebrospinal barrier and the in situ concentration of anti‐LeishmaniaIgGs and antigens in brain tissues may predispose to the pathological features detected in this

ISSN:0141-9838    

DOI:10.1046/j.1365-3024.1996.d01-28.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The immunogenicity of a yeast‐expressed 19 kDa fragment ofP vivaxMSP‐1 in the presence of different adjuvant formulations was evaluated. ICR mice were immunized with the 19 kDa antigen, using Freund's, alum, and block copolymer P1005 in water‐in‐oil (W/O) or oil‐in‐water (O/W) emulsions with or without detoxified lipopolysaccharide (RaLPS) as adjuvants. Five weeks following immunization with the antigen, mice were boosted with asexual blood‐stage antigens. Three weeks after the last immunization with the 19 kDa antigen, mice from the Freund's group and most groups that received P1005 as adjuvant had higher total IgG titres than those that received alum as adjuvant or antigen alone. Antibody responses after the antigen immunization were predominantly of the IgG1 isotype, but mice in the Freund's and P1005 (W/O or O/W emulsion with or without RaLPS) groups also had high titres of IgG2a and IgG2b. Antibody titres against merozoites increased in all groups after the parasite antigen boost. IgG2a levels in the group that received antigen in P1005 plus RaLPS in the W/O emulsion were higher than those receiving Freund's, alum or the other copolymer adjuvants. The high IgG2a titres in this group were associated with reduc

ISSN:0141-9838    

DOI:10.1046/j.1365-3024.1996.d01-32.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

A carbohydrate enriched soluble fraction (CHP) was prepared by mild treatment of viable Echinococcus granulosus protoscoleces (PSC) with the enzyme endoglycosidase‐F (endo‐F) and characterized by SDS‐PAGE, glycoside‐ inhibition ELISA, and immunoblotting. Three groups of four BALB/c mice were immunized with the CHP, with the remaining deglycosylated PSC (DGP) and with dead PSC (DPSC) to analyse the relative immunogenicity of carbohydrates on the surface of PSC. A fourth sentinel group was not immunized. The antibody response was analyzed during primary and hyperimmune responses. Specific antibody titres (IgG and IgM) against somatic PSC antigens (PSA) were evaluated by ELISA and their antigen recognition pattern by immunoblotting, discriminating carbohydrate and peptide specific antibody responses by periodate treatment of PSA. The avidity index of those antibodies and the titer of non‐specific immunoglobulins during the whole protocol were evaluated by ELISA. In vitro mitogenic activity of CHP was also evaluated. The results indicated: 1) immunodominance of surface carbohydrate epitopes from PSC, 2) predominant IgM and low avidity response against these epitopes, 3) a dramatic increase of non‐specific antibody titres and 4) in vitro mitogenic acti

ISSN:0141-9838    

DOI:10.1046/j.1365-3024.1996.d01-30.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The immunological reactivity ofEchinococcus granulosusantigen B was evaluated in 30 hydatid patients. Antigen B was purified from sheep hydatid cyst fluid by electroelution from a non‐reducing SDS‐PAGE gel (AgB). In ELISA and immunoblotting (IB), determining antibody production in sera from patients with hydatid disease and with other parasitic infections, purified AgB showed higher specificity than a partially purified antigen named pH5PPT (100%vs83% in pH5PPT‐ELISA and 58% in pH5PPT‐IB). AgB‐IB achieved higher sensitivity than AgB‐ELISA (80%vs63%). All AgB‐IB positive sera recognized the 12 kDa subunit. Qualitative AgB‐IB assessment of IgG isotype responses identified IgG4 as the predominant isotype (87%). The other isotypes showed a lower percentage of positive reactions: IgG1, 33%; IgG2, 21%; and IgG3, 17%. PBMC proliferative assay revealed a cellular response to AgB in 100% of patients’ PBMC. These findings confirm antigen B, especially its smallest subunit, as a good

ISSN:0141-9838    

DOI:10.1046/j.1365-3024.1996.d01-31.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY

摘要:

The aim of this study is to evaluate the effects of parasite clearance on the immunological profile of peripheral blood mononuclear cells (PBMC) from chagasic patients submitted to specific drug therapy. PBMC were examined by flow cytometry and proliferative responsiveness toTrypanosoma cruzi‐related stimuli. Three groups of patients were studied: not treated (NT), treated not cured (TNC) and cured (C). All data were compared to values from uninfected individuals (NI). NT displayed a lower percentage of CD3+cells as compared to NI, while TNC and C had mean values that were between those from NI and NT. Infected patients had double the percent of CD3+HLA‐DR+cells, independent of the efficacy of the treatment. Thus, absence of circulating parasites did not reduce T cell activation in Chagas' disease. NT displayed a higher percentage of CD5+B cells as compared to NI, while TNC and C had mean values between those from NI and NT. In contrast to the phenotypic data, thein vitromean proliferative responses to parasite‐related stimuli of PBMC from C were reduced to the low mean levels observed in NI. These striking differences were statistically different from the high responses seen in NT and TNC. Our data suggest that proliferative responses of PBMC from C reflect immunological changes due elimination of parasite. However, successful treatment did not alter the levels of peripheral T cell activ

ISSN:0141-9838    

DOI:10.1046/j.1365-3024.1996.d01-29.x

出版商:Blackwell Science Ltd    

年代:1996

数据来源: WILEY