摘要:

AbstractA high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20‐tetra(m‐hydroxyphenyl)chlorin (m‐THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20‐tetra(p‐hydroxyphenyl)clorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal‐to‐noise ratio of 5). The intra‐ and inter‐assay coefficients of variation (CV) on analysis of a plasma spiked with m‐THPC (1 μg/mL) were 2.3 and 3.4%

ISSN:0269-3879    

DOI:10.1002/bmc.1130070112

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractWe have investigated the hydrophobic properties of human skin fibroblast proteoglycans and related material by affinity chromatography on Octyl‐Sepharose CL‐4B in 4 M guanidinium hydrochloride (GdnHCI). Proteoglycans and related material could be separated into non‐, medium and highly hydrophobic forms by elution with gradients of Triton X‐100 in 4 M Gdn HCI. The non‐hydrophobic material included endogenously produced glycosaminoglycan chains and oligosaccharides as well as an HS‐proteoglycan with a 35 kDa core. The 65–70 kDa core (glypican‐related) proteoglycans appeared among the highly hydrophobic ones, but variable proportions were seen both in the medium and the non‐hydrophobic material. Other membrane‐bound proteoglycans, like fibroglycan (45 kDa core) and the HS‐proteoglycans with 90 and 130 kDa cores, as well as the CS/DS‐proteoglycan with a 90 kDa core, were all of high hydrophobicity. There were also indications of a highly hydrophobic CS/DS‐proteoglycan with a 45 kDa core. The extracellular proteoglycans, PG‐L, PG‐S1 and PG‐S2, and the HS‐proteoglycans with 350 and 250 kDa cores were all of medium hydrophobicity. These proteoglycans emerged in distinct positions when the column was eluted with a gradient of 3‐[(3‐cholamidopro

ISSN:0269-3879    

DOI:10.1002/bmc.1130070113

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractNovel fluorogenic Edman reagents, 7‐N,N‐dimethylaminosulphonyl‐4‐(2,1,3‐benzoxadiazolyl)isothiocyanate (DBD‐NCS) and 7‐aminosulphonyl‐4‐(2,1,3‐benzoxadiazolyl)isothiocyanate (ABD‐NCS) having no fluorescence themselves were synthesized. The derivatives of amino acids with DBD‐NCS fluoresce at 505 nm with excitation at 385 nm. They were separated on a reversed‐phase HPLC column and detected at the sub‐picomol level. Ala‐Phe derivatized with DBD‐NCS was cleaved by acid t

ISSN:0269-3879    

DOI:10.1002/bmc.1130070114

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0269-3879    

DOI:10.1002/bmc.1130070115

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0269-3879    

DOI:10.1002/bmc.1130070101

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY