摘要:

AbstractTotal opioid peptide receptoractivity in human cerebrospinal fluid is measured in patients who are experiencing lower back pain. Desalted CSF is eluted from a C18 Sep‐Pak and is subjected to a radioreceptorassay (RRA) that employs tritiated etorphin, which is aligand that is effectively displaced by opioids from several different types of opioid receptors. Three clinical groups have significantly different endogenous levels of 2.4, 4.5, and 6.4 pmol of methionine enkephalin‐equivalents per mL CSF. Those three levels indicate that more opioid activity is correlated with the amount of drug to relieve the patient's perception of pain. When the total opioid content exceeds an empirical threshold, the sample is further fractionated with gradient reversed phase HPLC, and the opioid receptoractivity in each HPLC fraction is measured to determine the characteristics pattern of those receptoractive opioid peptides present in that patient's CSF. Different HPLC RRA patterns are found for different clinical categories. A possible interpretation of these two different sets of data‐is that a lesion exists in one or several of the opioid peptidergic systems (metabolism, receptors) in this particular patient popul

ISSN:0269-3879    

DOI:10.1002/bmc.1130020202

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractAn HPLC method with polarographic detection for the trace determination of artemether in plasma and whole blood was developed and applied to pharmacokinetic and clinical pharmacological studies. The method showed high sensitivity and selectivity because of the easy reduction of the peroxide linkage of artemether at the mercury drop electrode. The detection limit was 10 ng and the detector response was linear over the range of 10 ng to 1 μg artemether injected onto the column. The largest relative standard deviation of 10 replicate measurements of standard solutions (concentrations of 10 ng/mL–1 μg/mL) was 8%. The recovery from whole blood and plasma of added drug (concentrations of 15–48 ng/mL) was 71

ISSN:0269-3879    

DOI:10.1002/bmc.1130020203

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractHuman insoluble elastin was prepared from newborn lungs and digested by leucocyte elastase. The soluble fragments were compared to those obtained from adult lung elastin in a previous work (Smyrlakiet al., 1986).Gel filtration on a Bio‐Gel P‐100 column of newborn elastin allowed the separation of fraction F1N (Mr's 30 000–10 000) which was eluted later than the excluded fraction F1A (Mr's 80 000–30 000) previously isolated from adult elastin. The difference in the sizes of the large peptide fragments originating from both elastins was also shown on SDS‐PAGE.Reversed phase HPLC was performed on a C18column using a multi‐step gradient elution procedure. Different patterns were observed for the high (F1N) and the low (F2N) molecular size fragments of newborn elastin. The same peak distribution was obtained with adult elastin. Comparison of the amino acid compositions of the most retained peaks (3, 4 and 5), derived from fractions F1N and F1A, showed analogies for the contents of the major nonpolar amino acids and crosslinks. Thus, this procedure allowed the separation of typical fragments of elastin which might be releasedin vivoby leucocyte elastase during pulmona

ISSN:0269-3879    

DOI:10.1002/bmc.1130020204

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractA simple and precise method has been devised for the quantitation of biliprotein (δ‐bilirubin or albumin bound bilirubin) in serum. In the presence of caffeine/benzoate, Bond‐Elut (C8, 200 mg) extracts unconjugated and conjugated bilirubin but not pigments that are covalently bonded to albumin which pass through the column and can be quantitated by a standard diazo method. Following elution from the Bond‐Elut column with methanol‐acetonitrile (50: 50, v/v) unconjugated and conjugated bilirubin can be quantitated either as total pigments or individually

ISSN:0269-3879    

DOI:10.1002/bmc.1130020205

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractExpired air samples have been analyzed from three groups of human subjects (normal, liver dysfunction, lung cancer) and the baboon (Papio anubus). Of the several hundred compounds present, three compounds were of particular interest due to their structural relationship to the isoprenoid‐type intermediates in the sterol pathway. These compounds were 1‐methyl‐4‐(1‐methyl‐ethenyl)‐cyclohexene, 6‐methyl‐5‐hepten‐2‐one, and 6,10‐dimethyl‐5,9‐undecadien‐2‐one. Hydroxyacetone was also found in all samples screened. The relationship of these compounds to the non‐sterol pathway of

ISSN:0269-3879    

DOI:10.1002/bmc.1130020206

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractA method utilizing HPLC to estimate ferrochelatase activity in human liver cells is presented. A partially purified homogenate of liver cells is incubated with mesoporphyrin IX and cobalt(II) ion. The ferrochelatase in the homogenate incorporates the cobalt(II) ion into the mesoporphyrin. After a fixed time (90 min) the porphyrins are extracted into an ethyl acetate‐acetic acid mixture. The porphyrins are then separated using reversed phase HPLC with a mobile phase of methanol‐acetonitrile‐phosphate buffer pH 3.0 (200:60:30 v/v). The enzyme activity is estimated by measuring the rate of utilization of mesoporphyrin. The optimum pH and substrate concentration for the reaction have been deter

ISSN:0269-3879    

DOI:10.1002/bmc.1130020207

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractHigh performance ion exchange chromatography was employed to evaluate the presence of short chain organic acids in the vaginal fluid of a woman troubled by persistent foul vaginal odor, but who did not have typical bacterial vaginosis. The vaginal secretions from this patient were collected on a weighed cotton swab and eluted into water and extracted by acidified ether. Salts of the acids were back‐extracted into aqueous solution and chromatographed on an H‐form resin column and compared to commercially available standards. A strikingly large amount of caproic acid was found. The caproic acid disappeared after metronidazole therapy, and a subsequent follow‐up chromatogram showed a predominance of lactic acid. The success of this technique in evaluating the present case suggests that such a method may prove useful in other types of vaginal infe

ISSN:0269-3879    

DOI:10.1002/bmc.1130020208

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractA universal extraction procedure is described for fat‐soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV‐detection of (α + β)‐carotene, vitamin E. (α‐tocopherol) and vitamin A (all‐trans‐retinol) in one single run, and of vitamin K1 (trans‐phylloquinone) and 25‐hydroxy vitamin D3 after sample clean‐up using disposable reversed‐phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat‐soluble vitamins can be performed in less than 5 mL of serum. Analyses of external quality control and randomly taken outpatient samples are shown to be of great value in assessin

ISSN:0269-3879    

DOI:10.1002/bmc.1130020209

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

ISSN:0269-3879    

DOI:10.1002/bmc.1130020210

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

ISSN:0269-3879    

DOI:10.1002/bmc.1130020212

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY