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1. |
My Journal Is Smaller Than Your Journal |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 231-232
Kenneth Arndt,
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ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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2. |
S‐100 Protein‐Negative Malignant MelanomaFact or Fiction?A Light‐Microscopic and Immunohistochemical Study |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 233-240
Zsolt Argenyi,
Carin Cain,
Christine Bromley,
Anh Nguyen,
Andrew Abraham,
Russell Kerschmann,
Philip LeBoit,
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摘要:
S-100 protein is considered a characteristic immunohisto-chemical marker for all nevomelanocytic lesions, in which it is expected to be present consistently. We reviewed 17 cases of malignant melanomas that previously tested negative for S-100 protein. They were reevaluated by light microscopy, a broad panel of immunohistochemical reagents including monoclonal and polyclonal antibodies to S-100 protein, and electron microscopy. On reexamination, five of the 17 cases were reclassified as nonmelanoma tumors, and eight of the 17 cases were found to be positive for S-100 protein (six with monoclonal and eight with polyclonal antibodies) and HMB-45 antigen, consistent with melanoma. The remaining four cases repeatedly tested negative for S-100 protein despite various antigen enhancement methods, but they were positive for HMB-45 antigen and contained premelanosomes or melanosome-like structures by electron microscopy. Two of these repeatedly S-100 negative melanomas were acrally located; although the numbers are small, a possible relationship to a specific anatomic location cannot be excluded. These findings suggest that in a small subset of melanomas S-100 protein is either not fully expressed or is below the level that can be detected by routine immunohistochemistry. We also conclude that in the majority of the initially S-100-negative cases of melanomas, the misdiagnosis may occur due to the use of an incomplete immunohistochemical panel, technical reasons, or the inherent variability of tissue expression of S-100 protein.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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3. |
Epithelial Markers in Malignant MelanomaA Study of Primary Lesions and Their Metastases |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 241-246
Ofer Ben-Izhak,
Paula Stark,
Rivka Levy,
Reuven Bergman,
Chaim Lichtig,
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摘要:
In order to determine epithelial markers in malignant melanoma in routinely processed paraffin sections and to compare the staining of primary (cutaneous) malignant melanomas and their metastases, we stained formalinfixed paraffin sections of 13 primary and 18 metastatic malignant melanomas using the streptavidin-biotin peroxidase method by antibodies to S-100, vimentin, HMB-45, polyclonal carcinoembryonic antigen (CEA), monoclonal CEA, cytokeratins (CAM 5.2 and broad-spectrum CKKES), and epithelial membrane antigen (EMA). All primary and most metastatic malignant melanomas showed positive staining with anti-S-100, HMB-45, and anti-vimentin. Reactivity with polyclonal CEA was observed in 15 (48%) of the 31 lesions; 14 of them were metastatic. No lesion was reactive with monoclonal CEA. Significant cytokeratin (CK) staining was evident in only three (9.7%) lesions (all metastatic), which also stained specifically with anti-CK 18. EMA was observed only focally in two (6.5%) lesions. There was no correlation between epithelial markers staining of the primary tumours and their metastases. All lesions with CK or EMA staining showed eoncomitant extensive staining for S-100, HMB-45, and vimentin. We conclude that (a) polyclonal CEA staining in malignant melanoma is not rare and is probably due to CEA-related molecules; (b) significant CK reactivity is rare and related to simple CK, such as CK 18; (c) epithelial marker reactivity is more common in metastases of malignant melanomas and is not correlated to the reactivity in their primary tumors. Considering our results and reports of positive S-100, vimentin, and HMB-45 in epithelial tumors, a wide panel of antibodies is recommended for the study of undifferentiated tumors.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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4. |
Karyometry of Primary and Metastatic Malignant MelanomaA Correlation with Cell Proliferation |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 247-252
C. Piérard-Franchimont,
G. Piérard,
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摘要:
We used computerized image analysis, immunohisto-chemistry, and the incorporation of tritiated thymidine to study the relationship between nuclear size and shape and the proliferation of malignant melanoma cells. A total of 30 nonulcerated primary malignant melanomas and 75 of their cutaneous metastases were considered. We found a positive relationship between (a) the3H-TdR labeling indices and the thickness of the primary malignant melanoma; (b) the nuclear profile areas of primary lesions and their respective metastases; and (c) the size and regularity of the contours of the nuclei. We failed to find a relationship between3H-TdR indices and the nuclear size of malignant melanoma cells.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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5. |
Cutaneous Collision TumorsAn Analysis of 69 Cases and Review of the Literature |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 253-257
Alan Boyd,
Ronald Rapini,
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摘要:
The association of two different neoplasms in the same biopsy specimen is not uncommon and has been reported by numerous authors. Some associations may result from involvement of related cell types, however, most are serendipitous. Our study attempted to investigate the nature and types of contiguous or “collision” tumors seen in our dermatopathology service. A retrospective evaluation of ∼40,000 cutaneous biopsies was performed, yielding 69 examples of collision tumors. Excluded were biphasic lesions of the same cell type (e.g., basosquamous carcinomas or combined nevi). Results were as follows: The most common combinations included basal cell carcinoma and nevus (14), basal cell carcinoma and seborrheic keratosis (8), nevus and seborrheic keratosis (14), actinic keratosis and nevus (7), and basal cell carcinoma and neurofibroma (4). Adnexal tumors were found combined with other neoplasms in four patients and blue nevi were noted in three additional tumors. We believe that most of these represent the presence of two or more common lesions juxtaposed by coincidence. However, certain combinations are of similar cell lineages or contain a relatively uncommon tumor and may indicate a possible link between the different cell types.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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6. |
Fibronectin, Laminin, Type IV Collagen Distribution, and Myofibroblastic Stromal Reaction in Aggressive and Nonaggressive Basal Cell Carcinoma |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 258-267
G. Rosa,
E. Barra,
M. Guarino,
S. Staibano,
V. Donofrio,
A. Boscaino,
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摘要:
The stromal myofibroblastic reaction and extracellular matrix in a series of 19 morphologically and clinically aggressive basal cell carcinomas (BCC) were examined by an immunohistochemical staining panel for myofibroblastic markers (vimentin, actin, and desmin), for basement membrane components (laminin and type IV collagen), and for fibronectin. Results were compared with a series of 19 nonaggressive BCC. The aggressive BCC showed discontinuous staining for laminin and type IV collagen, a marked myofibroblastic response, and increased stromal fibronectin. Conversely, the nonaggressive BCC showed a continuous basement membrane and a total lack of staining for fibronectin and myofibroblastic markers in all but three cases. This report suggests that a combined analysis of extracellular matrix components and myofibroblastic markers may be useful for evaluating the clinical behavior including the metastatic capability of aggressive BCC.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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7. |
Clinical Application of Polymerase Chain Reaction Amplification to Diagnosis of Herpes Virus Infection |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 268-274
C. Thomas,
Salli Smith,
Timothy Morgan,
Wain White,
Steven Feldman,
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摘要:
Amplification of viral DNA offers a potentially sensitive and specific method for identifying herpes viruses in pathologic specimens. The purpose of this study is to assess the value of polymerase chain reaction amplification of DNA as a diagnostic test for herpes virus in pathologic specimens. The purpose of this study is to assess for herpes virus infections. We examined 79 paraffinembedded tissue samples from 43 patients and 55 viral culture samples from 45 patients. Herpes virus DNA in the specimens was amplified by polymerase chain reaction. Using paraffin-embedded tissue on which a diagnosis of herpes virus was made by morphologic criteria, 11 of 19 patients had herpes virus DNA identified by PCR; herpes virus DNA was not identified in any of 35 negative control specimens. Herpes virus DNA was also identified by polymerase chain reaction in all of the positive herpes virus culture specimens. Of 29 culture negative specimens, herpes virus DNA was identified in six. We conclude that polymerase chain reaction is useful to establish or confirm the presence of a herpes virus infection in paraffin-embedded tissue samples and that it is more sensitive than viral culture.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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8. |
Human Eccrine Hamartoma of the Forearm‐Antebrachial Organ of the Ringtailed Lemur (lemur catta) A Possible Phylogenetic Relationship? |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 275-279
Daisy Kopera,
H. Soyer,
Helmut Kerl,
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摘要:
A 31-year-old woman presented with a clinically otherwise unsuspicious area of profuse sweating on her right forearm. Without triggering agents, sweating attacks producing a clear, serous fluid were observed daily. Histopathologic examination of a biopsy specimen showed hyperplastic eccrine glands with pale, stippled cytoplasm characteristic of eccrine hamartoma. No explanation, however, has been given for the fact that several authors observed eccrine hamartomas in the same anatomical location. Adolescent lemurs of the speciescatta(ringtailed lemur) are equipped with a pair of antebrachial cutaneous glands located on the volar surface of the wrist. They exude a clear secretion enabling them to “brachial branch mark” their territories. Histopathologic findings in the ringtailed lemur's antebrachial organ show characteristics of both apocrine and eccrine glands. In contrast to normal apocrine glands, however, the antebrachial organs of ringtailed lemurs reach the epidermis directly and are not connected to hair follicles. According to the “biogenetic law” of Ernst Haeckel, stating that ontogeny has to be seen as a short and incomplete repetition of phylogeny, a human fetus passes all evolutional stages from a single cell via amphibians and mammals to a human being. Thus, the antebrachial organ of the ringtailed lemur may be the “phylogenetic explanation” for eccrine hamartomas of the forearm in humans. The histopathologic findings of the antebrachial organ and of eccrine hamartomas are in accordance with this hypothesis.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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9. |
Multiple Nevoid Spiradenomas |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 280-284
Giuseppe Noto,
Maria Bongiorno,
Gabriella Pravatà,
Mario Aricò,
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摘要:
This report delineates a new clinical presentation of multiple benign spiradenoma (BS), a diffuse linear nevoid pattern. A 16-year-old girl, in otherwise good health, had many papulonodular lesions, varying in color from bluegray to bright red, disposed linearly on the right half of her body, involving the periorbital region, cheek, lateral side of the neck, trunk, linea alba, right thigh, and leg. Only a few nodules were slightly painful. Lesions were reported to be present at birth; no family members had similar lesions, either in solitary or multiple form. Excisional biopsies of three lesions from the neck, abdomen, and leg, respectively, were performed; histologically, all three samples showed a picture of BS. In some areas, the tumor was directly connected to a pilosebaceous unit. Furthermore, a focal area with pilar differentiation at the periphery of the lobules was observed, which might suggest that BS can differentiate toward various cutaneous adnexal structures. Our patient probably is the first one reported with diffuse linear nevoid lesions of BS, present at birth and involving the right half of the body.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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10. |
Primary Osteosarcoma of the Penis |
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The American Journal of Dermatopathology,
Volume 16,
Issue 3,
1994,
Page 285-287
Allan Sacker,
Kevin Oyama,
Stephen Kessler,
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摘要:
We report a case of an 84-year-old man with extraskeletal osteosarcoma of the penis arising within heterotopic bone. Extraskeletal osteosarcoma of the penis is an exceedingly rare entity; only two other well-documented cases have been reported in the English-language literature.
ISSN:0193-1091
出版商:OVID
年代:1994
数据来源: OVID
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