ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

In various tumors, proliferative activity has been demonstrated to be closely related to the degree of malignancy. In the present study, we examined the proliferative pool in a total of 25 melanocytic (nevocytic) skin tumors by immunohistochemical staining with Ki 67 monoclonal antibody (labeling the G1, S, G2-phase of the cell cycle) and quantitative morphological evaluation. There were highly significant differences in the numerical density of Ki 67–positive cells between nevocellular nevi (number of positive cells: 2.2 ± 0.7 x 103/mm3), primary malignant melanoma (6.3 ± 1.9 x 103/mm3), and metastatic malignant melanoma (47.0 ± 9.2 x 103/mm3). Within the malignant melanoma group, there was a significant correlation between proliferative activity and maximum tumor thickness (r= 0.85; p < 0.05). Comparison with previous studies using tritiated thymidine labeling and colony-forming efficiency shows that the stereological evaluation of Ki 67 staining in melanocytic skin tumors provides a reliable estimate of proliferative activity, which might be of prognostic value.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Computerized video image analysis of Feulgen-stained paraffin sections was used to assess the relationships of nuclear DNA content and other nuclear morphological characteristics with survival from cutaneous melanoma. The results indicate that nuclear area and deviation from diploidy have effects on survival independent of tumor thickness and the age of the patient. Tumor thickness, however, remained the most important predictor of survival. Video image analysis of tissue sections, by virtue of its speed, simplicity, and accuracy, appears to be a potentially valuable prognostic method.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

To define the histogenesis of the Paget cell and possibly identify differences in cells from the two sites, six vulvar and 23 mammary specimens from Paget's disease lesions were studied for immunocytochemical antigens. All vulvar and 21 (91%) mammary lesions were strongly reactive for glandular cytokeratin. All lesions showed immuno-positive Paget cells with epithelial membrane antigen (EMA). An apocrine antigen, gross cystic disease fluid protein (GCDFP-15), decorated 66.5% and 56.5% of extramammary and mammary lesions, respectively. All vulvar Paget cells stained for carcinoembryonic antigen (CEA), a frequency significantly greater than the 35% in mammary lesions (p= 0.02). However, CEA is expressed by both eccrine and apocrine sweat glands and their tumors. Vulvar Paget cells were negative for lysozyme, casein, lactalbumin, and S100 protein, compared with 9%, 4%, 4%, and 26% in nipple lesions. S100 protein expression is similar to that in mammary ductal carcinoma (32%). The glandular origin of both extramammary and mammary Paget cells is indicated by the presence of glandular cytokeratin, EMA, and CEA. Approximately 60% of all cases in both sites showed evidence of apocrine derivation (GCDFP-15 positivity). Variable antigen expression suggests possible malignant transformation of pluripotent germinative cells able to differentiate in an apocrine or an eccrine direction, or in both.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Histologic studies examining chronologic aging in skin have been confined to white skin. In the present study, we examined the features of sun-protected black skin from individuals 6 weeks to 75 years of age with light and electron microscopy. With age, the dermoepidermal junction became flattened with multiple zones of basal lamina and anchoring fibril reduplication. Microfibrils in the papillary dermis became somewhat more irregularly oriented. Compact elastic fibers showed cystic changes and separation of skeleton fibers with age. The area occupied by the superficial vascular plexus in specimens of equal epidermal surface length decreased from the infant to young adult (21–29 years old) to adult (39–52 years old) age groups, then increased in the aged adult (73–75 years old) age group. With the exception of the vascularity in the aged adult group, the above features are similar to those seen in aging white skin, and suggest that chronologic aging in white and black skin is similar. In addition, there was a decrease in the number of melanocytes with age. Basal keratinocyte melanin granule density increased with age to age 52 and remained dense in the aged adult group, even as the number of melanocytes decreased.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

The involvement of cellular immune reactions in the pathogenesis of many inflammatory dermatoses can be postulated from their histopathologic features, e.g., those dermatoses that show a perivascular lymphohistiocytic infiltration in the dermis in addition to their own characteristic epidermal changes. Several granulomatous dermatoses further show an additional infiltration of epithelioid cells. However, experimental production of similar changes is usually not feasible in animals. A human model is needed of delayed-hypersensitivity skin reaction induced by a definite antigen. After closely observing the histopathologic changes of the skin reactions to the purified protein derivative (PPD) of tuberculin, we noted that all patients did not show uniform histologic patterns at intradermal PPD injection sites. Therefore, we have tentatively analyzed histopathologically the pattern of tuberculin skin reactions at injection sites 48 h to 1 year after injection in eight healthy volunteers and 63 patients with various dermatoses. We classified the reactions at 48 h into three types, based on the extent of the tissue damage (i.e., their resemblance to features noted in corresponding inflammatory dermatoses): (a) the perivascular dermatitis type, (b) the basal spongiotic dermatitis type, and (c) the erythema multiforme type. There was a clear correlation between the magnitude of macroscopic skin reactions and these histopathologic patterns. Even those with clinically negative reactions showed a reaction pattern of perivascular dermatitis, whereas those who clinically developed prominent inflammatory reactions with blister formation always showed a erythema multiforme-like histologic pattern. Interestingly, in more than half of the cases, the dermal cellular infiltrate had a mixture of various numbers of neutrophils in addition to mononu-clear cells. Two-thirds of biopsy specimens taken after 14 days showed features of epithelioid cell granuloma as noted in tuberculosis, sarcoidosis, tuberculoid leprosy, and metal-hypersensitivity granulomas. In the remaining cases, however, there was only a monotonous mononu-clear cell infiltration similar to that described as typical delayed-type hypersensitivity reaction. Two biopsy specimens taken long after PPD injections, i.e., one after 4 months and another one after 1 year, respectively, showed palisading granuloma associated with collagen degeneration as noted in rheumatoid nodules. We think that the analysis of these model inflammation patterns of delayed-hypersensitivity reaction induced by intradermal PPD injection in adults provides a valuable clue for the elucidation of thus far unknown pathomechanisms of various inflammatory dermatoses that are presumed to develop based on cellular immune reactions.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

We studied a polypoid cutaneous neoplasm excised from the left arm of a 23-year-old man by light microscopy, immunohistochemistry, and electron microscopy. Histologically, the tumor was composed of a proliferation of round to oval epithelioid cells that were arranged in fascicles and showed a prominent perineural distribution. Immunohistochemistry revealed the presence of S-100 protein and vimentin within tumor cells. Electron microscopy demonstrated reduplication of basal lamina and abundant long-spacing collagen in intercellular spaces. The findings are consistent with a primary malignant epithelioid schwannoma of the skin. We discuss the relationship of this lesion with other neoplasms in the spectrum of tumors showing schwannian and melanocytic lines of differentiation.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

We report the case of a spontaneously regressing lymphocytic lesion on the neck of a 76-year-old man. Routine histopathological examination revealed a heterogenous infiltrate composed of both lymphoid follicles and preferentially perifollicular and interfollicular sheets of large atypical cells. Immunohistologically, large atypical cells were positive for Ki-1/Ber-H2 (CD30), anti-Il-2 (CD25), Ber T9, anti–HLA-DR, Leu-5b (CD2), T3 (CD3), Leu-3 (CD4), Leu-1 (CD5) and, occasionally, Leu-M1 (CD15); that is, they showed an arrangement and immunopheno-type similar to those of perifollicular Ki-1 positive cells in reactive lymphoid tissue. In contrast, antibodies To15 (CD22), anti-immunoglobulins, and anti-CALLA (CD10) stained polyclonal B-cells restricted to lymphoid follicles and small clusters representing remnants of follicles. We suggest that this lesion be termed “follicular lymphoid hyperplasia of the skin with high content of activated T-helper cells.”

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

A malignant dermal cylindroma of the scalp arose from one of multiple long-standing dermal cylindromas in a 76-year-old man with coexisting trichoepitheliomas and bilateral dermal analogue tumors of the parotid gland. The histologic transition from a benign dermal cylindroma to an anaplastic keratinocytic neoplasm was readily apparent. The malignant dermal cylindroma is a rare neoplasm. To our knowledge, the constellation of benign and malignant dermal cylindromas, multiple trichoepitheliomas, and salivary gland neoplasms has not been previously reported.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Two pyogenic granulomas arising from the wall of an epidermoid cyst on the midback of a 62-year-old white man are reported. Histologically, each pyogenic granuloma was a polypoid sessile nodule that showed well-vascularized granulation tissue; edema; a mixed inflammatory infiltrate; an epidermal collarette; and a thinned, variably ulcerated epidermis. Although pyogenic granulomas may develop anywhere on the skin, this appears to be the first report of pyogenic granulomas in an epidermoid cyst.

ISSN:0193-1091    

出版商:OVID    

年代:1989

数据来源: OVID