摘要:

The purpose of the present study was to determine if a nonmalignant skin-associated T-cell might exhibit nuclear irregularity and represent the normal counterpart to the malignant Sézary T-cell found in mycosis fungoides (MF) and the Sézary syndrome (SS). Punch skin biopsies from ten benign lymphoid infiltrates of skin were subjected to ultrastructural morphometric analysis and quantitative immunohistochemistry (on serial frozen sections) using a battery of monoclonal antibodies. Form factor (FF) (4tA/p2) was used to assess nuclear contour irregularity. The lymphoid infiltrates were morphometrically heterogeneous (range of FF values: 0.482–0.704). Immunophenotypically, there was marked variation in host response to the variety of antigens presented. Linear regression analysis was used to determine if nuclear contour irregularity showed any statistical relationship to immunophenotypically defined lymphocyte subpopulations. It was determined that nuclear contour irregularity (mean FF values) did not correlate with the presence of any surface antigen tested. This included the antigens Leu-2a, Leu-3a + b, Leu-4, Leu-6, Leu-7, Leu-8, Leu-9, Leu-14, and la. There was, however, significant correlation of increased nuclear contour irregularity with the presence of Leu-9−and Leu-8−cells (r = 0.7 and 0.6, respectively), lymphocyte subsets reportedly deficient in MF and the SS. This finding leads to the speculation that the Leu-9−, Leu-8−reactive T-cell with an irregular nucleus might represent the normal counterpart to the malignant clonally expanded T-cell found in MF and the SS. It was also determined that helper to suppressor ratios varied 68-fold from 0.2 to 13.5 among these ten benign lymphoid infiltrates of skin. This finding underscores the futility of using helper to suppressor ratios as a diagnostic tool in defining T-cell malignancies.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

Secondary alteration of eccrine sweat glands is frequent in neoplastic and inflammatory skin diseases. The histological changes are characterized by a remarkable variability. However, a systematic classification of these reactions does not exist. We examined routine histological sections of biopsy specimens and found morphological alterations of eccrine sweat gland in the secretory coil, the duct, and the surrounding connective tissue. Dilation with or without retention was the most common histological variation seen in the secretory segments and the ducts. In association with dilation, flattening of the secretory and ductal cells occurs. Other cell changes were hydropic alteration and vacuolization of the secretory cells; seldom was a thickening of the cuticle observed. A striking phenomenon was the proliferation of ductal epithelium in different skin tumors. We found histological changes of the surrounding connective tissue less frequently: marked periglandular fibrosis and mucinous degeneration occurred.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

Syphilis is still a common disease in many countries. The clinical features of secondary syphilis are well documented, and the histopathological features of secondary syphilis have been reviewed in a few studies. The present study documents the histopathology of secondary syphilis based on 68 skin biopsies from 38 patients. Based on the findings of this study, and a review of the literature, diagnostic guidelines are suggested and the differential diagnoses are discussed.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

Standard techniques used to study Langerhans cell (LC) populations are tedious and time-consuming. We developed a rapid and comprehensive method to evaluate LC using automatic digital image analysis. Human epidermal sheets were stained with OKT6 monoclonal antibody and evaluated on an IBAS 2000 computerized automatic digital image analysis system. The fields were imaged on a Photomicroscope I using a high-resolution monochrome video camera. Each field was digitized, normalized, and filtered. The LC were discriminated by density; each field was interactively edited to separate overlapping cells and to bridge small gaps in the dendrites. The discriminated binary image of the LC was measured for number of cells per field, total area of individual cells per field, and percent of the field occupied by LC. The cell bodies were then separately discriminated and subtracted from the thinned cell image to allow a separate measurement of number and length of dendrites per cell. Finally, the binary cell image in a reference section was dilated to occupy approximately the total field area. This degree of dilation was then applied to all subjects to give the “area of influence.” This methodology provides a rapid and comprehensive tool for the evaluation of LC.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

Topical application of citral on male rat skin induces hyperplasia of the sebaceous glands. Ultrastructural study showed that this hyperplasia is manifested by an increase in number of the partially differentiated cells of the gland. Citral was found in fat droplets of the mature differentiated and partially differentiated cells. Because citral causes an increase in testosterone level, we conclude that the sebaceous gland hyperplasia is related to androgen activity.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

Cantharidin has been used to induce acantholysis in vivo. Intraepidermal clefting starts at the junction between the basal and epidermal layers, and later involves all of the levels of the stratum spinosum. As a response to early acantholysis, an increased number of epibasal cells synthesize DNA. This is not related to an increased binding of epidermal growth factor to these cells. In sum, the basal-epibasal junction is one of the weakest within the epidermis. The epibasal cells represent a reserve pool for proliferating keratinocytes that may be stimulated without necessarily binding an increased amount of epidermal growth factor.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

A 60-year-old man was followed for 5 years for plaque stage mycosis fungoides (MF), follicular mucinosis (FM), and multiple recurrent acquired epidermal cysts on the face, scalp, right axilla, and inguinoscrotal areas. Topical nitrogen mustard and steroids, as well as systemic retinoids controlled the infiltrated lesions of MF, but had no effects on the cysts, which had to be removed surgically. Such cases have to be distinguished from MF in a tumor stage and may suggest that the recurrent cysts represent a paraneoplastic syndrome.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

A case of trabecular carcinoid of the skin of an 80-year-old man is described. This tumor's structure is totally different from that of trabecular carcinomas as described by Toker and Tang and Toker. It is formed by long, irregularly branching, serpiginous trabeculae, which are Grimelius positive and chromogranin immunoreactive. This appears to be the first case of trabecular carcinoid reported in the skin. An adnexal origin for this tumor is suggested.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

A malignant blue nevus of the right upper arm with hematogeneous lung metastases is presented. Histological examination showed that the tumor was composed of spindle, dendritic, and globular cells with hyperchromatic and polymorphic nuclei, atypical mitoses, and tumor cell necrosis. There was no proliferation of atypical melanocytes at the dermoepidermal junction. Histologically, malignant blue nevus should be distinguished from benign cellular blue nevus, primary cutaneous malignant melanoma, cutaneous metastases of malignant melanoma, and clear cell sarcoma.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

A disease resembling junctional epidermolysis bullosa in humans is described in a toy poodle. Shortly after birth, the affected animal developed vesicles and bullae on the pads of the feet and the mucous membranes of the oral cavity. The lesions rapidly increased in number and severity, eventually involving the glabrous skin of the ventral abdomen. Due to the severity of the lesions, the animal was euthanized when it was 48 h old. Histopathologic, ultrastructural and immunohistochemical evaluation defined a minimally inflamed subepidermal vesicular disease with separation occuring at the zona lucida of the basement membrane zone. Laminin and type IV collagen were present at the base of the vesicle.

ISSN:0193-1091    

出版商:OVID    

年代:1988

数据来源: OVID