ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03506.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03507.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03508.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

We describe here the CDR3s of a collection of monoclonal antibodies (MoAb) with specificity for the carbohydrate dextran B512 produced in the mouse strain C57BL/6. In spite of the postulated mechanisms for variability in this region, a high proportion of these monoclonals displayed identical VHD (24/30) and DJH(21/30) junctions and 21 of them were identical in the whole CDR3. These 21 independently generated identical CDR3s could be ordered in eight groups indicating that not a particular CDR3, but instead the mechanism for generating identical junctions was preserved. Two of the CDR3s in this study were found to be identical to the CDR3 of the monoclonal B1‐8 produced in C57BL/6 in response to proteins bearing the hapten (4‐hydroxy‐3‐nitrophenyl)acetyl (NP). This and other parameters support the notion that the generation of identical junctions could be independent of antigenic selection. We also report here the association between JHusage and amino acid (aa) residues at the VHD and DJHjunctions. Since these MoAb were generated in response to dextran B512, immunoglobulin conformation has to be compatible with antigen binding. Nevertheless, no aa residue of CDR3 could be directly related to antigen binding. We postulate therefore, that the observed selection of CDR3s could be directed to the production of variable regions with protein configuration most suitable with immunoglobulin folding and may occur prior to antigenic selection. Selection for junctional residues in relation to JHusage and the generation of identical CDR3s are probably different events. Possible genetic mechanisms operating for CDR3 construction and/or selection by cellular ligands are di

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03509.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

T cells from tumour infiltrating lymphocytes (TIL) cultured in media containing IL‐2 were shown to mediatein vitroandin vivoantitumour responses. To characterize the T‐cell antigen receptor (TCR) Vβ expression in autologous cytotoxic effectors we isolated CD3+CD8+CD4−cells from cultures of TIL and tumour‐associated lymphocytes (TAL) and analysed the TCR Vβ repertoire of CD3+CD8+CD4−lines of known HLA‐A, ‐B and ‐C phenotype, using polymerase chain reaction (PCR). These lines showed preferential lysis of autologous tumours and lysed, to a much lesser extent, NK and LAK cellsensitive targets. Tumour lysis was inhibited by antibodies to CD3 and MHC class I antigens indicating that they are cytotoxic T lymphocytes (CTL). These CD8+CTL lines expressed a broad distribution of TCR Vβ repertoire which was dominated by particular groups of Vβ families in each CTL line. However, no predominant expression of one or the same Vβ segment in all CTL lines was observed although statistical correlations between Vβ family usage and magnitude of the antitumour cytolytic response were found. These results suggest that certain TCR Vβ families may be selected by antigen in ovarian tumour‐reactive T cells and this selection may be affected by Ag expression, and/or host factors. To our knowledge, this is the first documentation of TCR Vβ repertoire of human ovarian tumour‐reactive CD3+CD8+CD4−CTL from different i

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03510.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Between January 1991 and January 1993, 265 patients who fulfilled the CDC criteria of the working case definition of Chronic Fatigue Syndrome (CFS) have been observed at our Institution and submitted for clinical and laboratory evaluation. One hundred and sixty‐three patients were females and 102 males, the median age was 35 years (range 4–55 years); all patients reported profound and prolonged fatigue, lasting for a median of 3 years (range 6 months–10 years), preceded or accompanied at appearance by fever in 185 cases, and neuropsychologic problems including inability to concentrate, difficulty in thinking, confusion, irritability, forgetfulness, and depression. The fatigue was so severe that it required 102 patients to stop their working activities for a period of time ranging from 3 months to 2 years (range 7 months). In 40 consecutive patients a comprehensive immunologic testing by single and two‐colour flow cytometry was performed and results compared with a group of 35 healthy, age‐ and sex‐matched controls. Whilst no significant differences were found in the absolute numbers of circulating total T cells (CD3+) and of total helper/inducer (CD4+) or suppressor/cytotoxic (CDS+) T cells, an evident reduction in CD3−/CD16+and CD57+/CD56+NK lymphocytes along with an expansion of the CD8+/CD56+and CD16−/CD56+NK subsets, were found in the CFS group. In addition, CD56+NK cells from CFS subjects were found to express an increased amount of cell adhesion molecules (CD11b. CD1 1c, CD54) and activation antigens (CD38). Both the percentage and absolute numbers of CD4+T cells bearing the CD45RA antigen appeared significantly reduced in CFS patients, and CD4+T lymphocytes from CFS subjects displayed an increased expression of the intercellular adhesion molecule‐1 (ICAM−1/CD54). Finally, the total numbers of circulating (CD19+) B lymphocytes, were significantly higher in CFS cases than in controls, and in 11 out of 30 CFS patients the increase in circulating B cells was sustained by the expansion of the CD5+/CD19+subset of B lymphocytes. We conclude that CFS is a syndrome not previously described in Italy, with already known clinical characteristics and appears to be associated with several immunologic abnormalities, including those reported previously in cohort of patients from different countries. We also show for the first time that CD56+NK cell subsets from CFS patients display an abnormally increased expression of cell adhesion molecules and

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03511.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

To investigate the role of synthetic polypeptide carriers in inducing an epitope‐specific immune response relevant for vaccine design, peptides comprising two distinct regions of herpes simplex virus type I glycoprotein D (1–23 and 273–284) have been conjugated to the branched polypeptides with polylysine backbone, poly[L‐Lys‐(DL‐Alam)] (AK), or poly[L‐Lys‐(LeUi‐DL‐Alam)] (LAK) and to keyhole limpet haemocyanin (KLH). The magnitude, fine specificity and isotype distribution of the conjugate‐, peptide and carrier‐specific antibody responses were characterized in immunized BALB/c and CBA mice. Conjugates containing the polypeptide carrier AK were the most effective in inducing HSV gD‐peptide specific antibody responses while KLH peptide conjugates resulted in conjugate‐specific antibody responses without measurable peptide specificity. The efficacy of AK‐peptide conjugates was verified by the dominant appearance of peptide‐specific antibodies belonging to functionally efficient IgG isotopes, accompanied by low levels of carrier specific antibody responses. Preimmunization of BALB/ or CBA mice with AK conjugates comprising the 1–23 or 276–284 HSV peptides resulted in prolonged survival of animals infected with a lethal dose of infectious HSV‐1. The potency of these conjugates in eliciting a protective immune response shows a close correlation with the relative levels of conjugate‐induced virus specific antibodies and the neutralizing activity of ser

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03512.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Consecutive sera from before and after treatment were collected from 23 patients with aSchistosoma mansoniinfection, seven of whom had an early infection. All sera were analysed for IgGl, IgG3 and IgG4 antibody activity against three peptides from the protein Sm 28 GST (24–43, 115–131 and 140–153 aa). In addition, sera from 14 patients, four with an early infection and 10 patients with a chronic infection, were analysed for IgG and IgA antibody activity using seven peptides derived from the protein Sm 28 GST. This molecule has previously demonstrated protective activity against infection in various experimental models.The results are indicative of a subclass‐related epitope specificity of the antibodies. Moreover, reactivity to one of the peptides (158–175 aa) was significantly associated with a chronic infectio

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03513.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Epidemiological and experimental evidence suggested that denial of dietary cow milk protein early in life protects genetically susceptible children and animals from insulin‐dependent diabetes (IDDM). Bovine serum albumin (BSA) was proposed as a candidate milk‐borne mimicry antigen responsible for the diabetogenic cow milk effect. Elevated anti‐BSA antibodies have been observed in patients and diabetic rodents, and these antibodies precipitate p69 from islet cell lysates. IDDM is a T ceil mediated disorder but efforts to detect BSA‐specific T cells in diabetic children have so far failed. We describe here a culture system which allowed the detection of BSA‐specific T cells and we mapped this response to the ABBOS peptide (pre‐BSA position 152–169) previously identified as a possible mimicry epitope. ABBOS sensitized T ceils were found in 28/31 children with recent onset TDDM but not in non‐diabetic controls nor in children with SLE or JRA. T cell proliferative responses declined within the first few years of diabetes diagnosis. Although no effector cell role for BSA/ABBOS specific T lymphocytes has been demonstrated, the presence of BSA peptide‐specific T cells strengthens the postulated link between a cow milk

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03514.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

The major surface protease ofLeishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified fromL. majorpromastigotes. A panel of human T‐cell clones recognizing this protein were generated from individuals who had previously had self‐healing cutaneous leishmaniasis. The T‐cell clones expressed CD4, and the alpha chain of the T‐cell antigen receptor. Gp63 reactive T‐cell clones activated by antigen or by immobilized anti‐CD3 antibody released relative large amounts of interferon‐gamma and no or little interleukin‐4, thereby resembling Thl cells. Autologous mononuclear cells and Epstein‐Barr virus‐transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages byL. major. The data confirm that human CD4+T cells recognizing gp63 can take part in the host defence agains

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1994.tb03515.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY