ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02344.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Primary hepatocyte cultures synthesize apo‐SAA upon stimulation with supernatant from lipopolysaccharide (LPS)‐treated macrophages. The matrices on which the hepatocytes were grown influence their basal apo‐SAA synthetic capability. Fibronectin was superior. Coculturing hepatocytes with hepatic sinusoidal cells did not adversely affect the ability of hepatocytes to synthesize and secrete apo‐SAA into the culture medium. In 72 h, clear islands of endothelial cells nestled in layers of hepatocytes. Both apo‐SAA, and apo‐SAA, were made in considerable quantities but no evidence could be obtained that the apo‐SAA were free of apo‐A‐1. The coculturing of hepatocytes with liver sinusoidal cells, the site of ultimate AA deposition, is a first step in establishing an in vitro system for A

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02345.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

L929, 3T12‐3, B16, 3LL, and YAC1 cells with cytostatic factor (CF)‐inducing activity fromLactobacillus casei‐elicited murine peritoneal macrophages (LCEPM) were susceptible to the cytostatic activity of LCEPM and to LCEPM‐produced CF, but L1210, P388D1, and Colon 26 cells, which have no CF‐inducing activity, were resistant to that of LCEPM and to the CF. The resistance of P815 cells to that of LCEPM was stronger than that of 3T12‐3 cells, but the CF‐inducing activity of P815 cells was about 5098 weaker than that of 3T12‐3 cells. Release of CF from LCEPM was also caused by heat‐killed (100°C, 10 min) 3T12‐3 or P813 cells, and this release was inhibited byd‐mannose. The CF‐inducing activity of heat‐killed 3T12‐3 or PK15 cells was reduced by mild trypsin digestion (37°C for 10 min). Ad‐mannose‐containing glycopeptide or glycoprotein (GP) was separated from 3T12‐3 or P815 cells by concanavalin A (Con A) or wheat germ agglutinini (WGA) affinity chromatography. The CF were released from LCEPM by stimulation with the Con A‐binding GP of the tumour cells, but the WGA‐binding GP had little activity.It is suggested that tumour cells with CF‐inducing activity may he susceptible to the cytostatic activity of LCEPM, and those without CF‐inducing activity may be resistant to the cytostatic activity of LCEPM and the release of CF from activated macrophages may be caused by the

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02346.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A large granular lymphocyte (LGL) leukaemia cell line from the Fisher/F344 rat strain called RNK‐16 has been established in vitro, maintaining the same surface markers as the tumour cell growing in vivo. The tumour has also maintained its specificity pattern and cytotoxic reactivity and serves as a suitable source of natural killer (NK)‐like effector cells in vitro. The cells show no evidence of dependency on, or production of, interleukin 2 or interferons, not is the cytotoxic capacity influenced by treatment with mitogens. The in vitro line does not produce natural killer cytotoxic factor (NKCF) in a constitutive manner, hut can be induced to do so via coculture with tumour target cells. When the fine specificity patterns were analysed, the RNK‐16 cells express species‐preferential lysis of susceptible target cells and a highly discriminatory power to kill only 1 out of 5 rat erythroleukaemia cell lines. When testing normal target susceptibility patterns. RNK‐16 kills lymphoblasts of B type better than T blasts, which is well in line with previous findings on normal NK cell specificity

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02347.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The immunogenicity of the minor histocompatibility antigen Eag‐1 of the rat kidney endothelium was studied in renal allografts mismatched for antigens of the major histocompatibility complex (MHC). The rejection rates of BN.1L (RT11, Eag‐1+), (BN.1L × MAXX)F1 (RT1Vn, Eag‐1+), and LEW (RT11, Eag‐1−) kidneys transplanted into unsensitized, bilaterally nephrectomized MAXX (RT1n, Eag‐1−) recipients were comparable, indicating that incompatibility for Eag‐1 has no effect on the survival of MHC‐incompatible kidney grafts. Transplantation of BN (RT1n, Eag‐1+) and WKY (RT1k, Eag‐1+) kidneys into unilaterally nephrectomized MAXX recipients led to a weak and inconsistent antibody response against Eag‐1, indicating that MHC incompatibility does not influence the formation of

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02348.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The tissue distribution of the receptor for platelet‐derived growth factor (PDGF) was investigated by immunohistochemistry on frozen sections from normal and inflamed synovial tissue using monoclonal antibodies to the receptor. Non‐inflamed synovial tissue showed no staining, indicating that PDGF receptor expression is low or absent in normal tissue. In contrast, tissue from synovitis with prominent neovascularization showed a strong staining in the tunica media of the proliferating blood vessels as well as on connective tissue cells in the stroma. Tissue from synovitis with prominent proliferation of synovial lining showed intense staining for PDGF receptors in fibroblast‐like cells of the lining and a less intense staining on vascular and connective tissue cells deeper in the stroma. Staining for PDGF receptors was also intense in the pannus tissue close to infiltrated bone and cartilage. In all these forms of synovitis, PDGF receptor staining was associated with increased HLA‐DR staining and infiltration of macrophages and T lymphocytes. The finding that PDGF receptor expression is induced in conjunction with the chronic synovial inflammation associated with rheumatoid arthritis and some other forms of arthritides suggests that PDGF may play a role in the stimulation of mesenchymal cell proliferation that often accompanies chronic inflammatory

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02349.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

We have found autoantibodies in the sera from rheumatoid arthritis (RA) patients which recognize two cell surface antigens of approximately 70 kDa and 28 kDa from synoviocyte extracts as detected by immunoprecipitation analysis. These polypeptides were immuno‐precipitated from extracts containing mainly macrophage‐like synoviocytes (type A) but not from extracts of homogeneous fibroblast‐like synoviocytes (type B). These autoantigens are not selectively expressed by RA synoviocytes, since both RA and non‐rheumatoid synovia were reactive for RA sera. From the panel of different RA sera tested, 64% immunoprecipitated the 70 kDa band, and 27% recognized the 28 kDa polypeptide. These differences in the specificity of the sera seemed to be related to the clinical state of the donor. The sera from patients suffering from other autoimmune diseases such as autoimmune thyroiditis and systemic lupus erythematosus (SLE) do not appear to be reactive for these specificities, but sera from patients with Sjögren's syndrome, psoriatic arthritis, and Crohn's disease showed a weak cross‐reactivity with the 70 kDa polypeptide. This autoreactivity against synovial cells in RA supports the idea that these cells participate in the initial immune response of t

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02350.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Intrasplenic immunization with antigen immobilized on nitrocellulose (NC) paper results in an immune response with nanogram amounts of antigen without the use of Freund's adjuvant. Comparing the sensitivity of the intrasplenic immunization with intraperitoneal or subcutaneous administration, we found that the intrasplenic method resulted in more positive animals with higher titres than did the other techniques with the smallest amount of antigen (70 ng of bovine serum albumin). With larger amounts of albumin the intraperitoneal method yielded the highest titres. No loss of antigenicity was observed when we immunized with equal amounts of protein separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), immunoblotted and stained with either Coomassie brilliant blue R 250 or Amido black 1

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02351.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Proliferative response of splenic T cells of C57BL/6 mice to mutant major histocompatibility complex (MHC) class I antigen (H‐2Kbm1) was examined with regard to the role of accessory cells. T cell proliferation in mixed lymphocyte culture (MLC) was not induced when accessory cells were removed from stimulator spleen cells by passage through Sephadex G‐10 or nylon‐wool column. Anti‐Iabantibodies did not inhibit the proliferative response to class I antigen, whereas the same antibodies completely blocked the response to class II antigen (Iabm12). Accessory cells may not be mere presenters of MHC class I antigen because stimulator cells fixed with 0.05% paraformaldehyde lost the stimulating function. The proliferative response was partially recovered by the addition of recombinant interleukin 1 (IL‐1) and/or IL‐2 to MLC devoid of stimulator type accessory cells. It is concluded that stimulatory type accessory cells were obligatorily involved in the T cell proliferation, and the production of IL‐1 by accessory cells is thought to play a critical role i

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02352.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Activation products of the complement cascade contain neoepitopes that are not present in the individual native components. Monoclonal antibodies detecting neoepitopes have been used for direct quantification of activation at different steps in the cascade. These methods are suggested to be more sensitive and reliable than conventional complement activation tests, which are hampered by precipitation or fractionation procedures. The present study describes production screening and characterization of a monoclonal antibody (MoAb) bH6, MoAb bH6 exhibited a significantly higher binding capacity to ELISA plates coated with zymosan‐activated human serum than to plates coated with EDTA plasma. When fixed to the enzyme‐linked immunosorbent assay (ELISA) plates, MoAb bH6 retained material from zymosan‐activated serum that only reacted with anti‐C3 antibodies. Crossed immunoelectrophoresis performed on zymosan‐activated serum demonstrated that MoAb bH6 co‐precipitated with anti‐C3c antibodies. In experiments using highly purified cell‐bound fragments MoAb bH6 showed reactivity with C3b and iC3b, but not with C3d. MoAb bH6 reacted in ELISA with purified C3c, but not with C3dg, both as capture antibody and in tests with the fragments absorbed to the solid phase. Thus, MoAb bH6 is highly specific for a neoepitope of human C3 expressed on the cleavage fragments of C3b

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02353.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY