ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03007.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03008.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03009.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

All ideas implicit in the papers since 1953 involved in applying mass‐action thermodynamics to antibody‐antigen reactions are unified by the use of: (a) the intermediary concept of extent of reaction; (b) the concept of intrinsic association constant; (c) a statistical analysis for probable complexes; and (d) identification of the complex or complexes that contribute to the bioassay. Several general theoretical examples are given that show the limitations of linear interpretations of equilibrium data. Two practical examples from the literature illustrate foot‐and‐mouth disease virus and influenza virus neutral

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03010.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

μ‐chain protein GLI is a pentameric molecule with an amino‐terminal deletion comprising 130 residues. The half‐cysteine residue (position 140) which forms the H‐L disulfide bridge in normal IgM is present. Instead of being joined to the L chain, it presumably exists as an additional inter‐H‐H disulfide bridge. The χ Bence Jones protein is of normal size and present in two forms: as monomers and dimers. The carboxy‐terminal half‐cysteine of the monomer is bound to cysteine. Possible reasons for failure of assembly between μ and L chains are

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03011.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In a search for H‐ or L‐chain‐related cross‐idiotypic specificity among human anti‐I and anti‐i cold agglutinins, two idiotypic antisera raised against the IgMχ cold agglutinin Da were tested for their binding activity to isolated cold agglutinin H and L chains. Negligible H‐chain binding activity was found, but there was high‐titre L‐chain binding activity in one of the antisera. This was an unsuspected VχIII subgroup activity which enabled the classification of VχIII proteins into three subgroups. The χ chains of five out of six anti‐I and anti‐i cold agglutinins belonged to the antigenically most active VχIII subgroup. Absorption of the idiotypic antiserum with a Bence Jones protein of this latter subgroup did Dot appreciably alter the precipitating cross‐idiotypic activity of the antiserum when tested with intact cold agglutinins. However, these studies do not rule out the possible existence of a VχIII subgroup‐associated conformational antigen in an intact Fab region, which is seen as a ‘cross idiotypic’ antigen by hetero

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03012.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Reduced and alleviated monoclonal IgM was fractionated into μ and light (L) chains by gel chromatography in 1N acetic acid. Equimolar mixtures of the chains formed a noncovalently bonded structure in 0.01M sodium acetate buffer, pH 4.1, that had the properties of a half subunit. The latter reassociated into a subunit‐like structure after transfer into 0.08M sodium phosphate buffer, pH 7.5. The similarity of the reconstituted IgM subunit (IgMs) to that of the native molecule was established by its physicochemical and immunochemical properties. Comparable products were obtained on reassembly of the alkylated μ and L chains from several other monoclonal IgM. The presence of active binding sites for IgG on subunits reconstituted from the chains of proteins with anti‐IgG activity further indicated correct assembly of the μ and L chains. High yields of subunit‐like products were also obtained by assembly of μ chains from one protein and L chains from another. Evidence was obtained that L chains of appropriate specificity can substitute for the homologous chain in the formation of the active site. Heterogeneous mixtures of high molecular weight products were generated from μ and L chains that were not alkylated. Reduction and alkylation demonstrated that the products represented polymers of reconstituted IgMs. Significant levels of anti‐IgG activity were detected in the polymeric IgM generated from the chains of active proteins by precipitation with ag

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03013.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Two of twenty IgA myeloma proteins studied were found to lack J chain. Both IgA proteins contained dimers and higher polymers (trimers, tetramers, pentamers) in proportions similar to those found in most classical ‘J‐positive’ proteins. Both the ‘J‐negative’ proteins contained bound albumin and alpha‐1 anti‐trypsin (α1AT), and reduction with mercaptoethylamine caused a release of albumin and α1AT concomitant with depolymerization of the higher polymers of IgA. These proteins formed complexes with secretory component (SC) in vitro, indicating that the presence of J chain is not a requireme

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03014.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Extrinsic Cotton effects generated by binding haptens to native and reformed MOPC‐315 protein, its subunits, and its Fv fragment have been examined. The identity of the combining sites of native and reassociated proteins and Fv‐315 was demonstrated by the identity of their circular dichroism (CD) difference spectra. The spectrum of TNP‐aminocaproate complexed with L chains differed in maxima and minima and cross‐over points and lacked the 495‐nm CD peak of TNP‐aminocaproate‐MOPC‐315 protein and Nα‐TNP‐tryptophan spectra. A negative 293‐nm tryptophanyl CD band, present in spectra of MOPC‐315 proteins and Fv‐315 but absent from spectra of L and H chains, was blue‐shifted by haptens and may represent electronic interactions occurring within the MOPC‐315 combining site between tryptophanyl and chromophoric residues of different subunit. This conclusion is supported by molecular models

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03015.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Human immune antibodies such as anti‐Rh and anti‐Kell antibodies were tested in hemagglutination and hemagglutination inhibition experiments for VHsubgroup composition. A striking VHsubgroup restriction was found in several of these groups of antibodies. In the majority of cases there was a restriction to one particular VHsubgroup for a single antibody specificity in a given individual. In some cases there was also an overall restriction to one particular subgroup for antibodies with the same antibody specificity. This was particularly pronounced for anti‐D antibodies, which were primarily restricted to VHII, and for the anti‐Kell, which was particularly related to VHIII. Subgroup‐specific antigens for all the main VHsubgroups were blocked on combination of the antibody molecule with antigen. No relation was found between VHrestriction and restriction to IgG subclass, or genetic markers or χ and λ light

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1976.tb03016.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY