ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02375.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A multiparameter study of malignant lymph node cells and peripheral blood lymphocytes of seven patients with peripheral T cell lymphoma is presented. The results of monoclonal marker studies showed three cases of helper‐suppressor T cell lymphoma (OKT4+, OKT8+), one case of suppressor T cell lymphoma (OKT8+), and three cases of helper T cell lymphoma (OKT4+). Immunophenotypic heterogeneity of neoplastic T cells with expression of pan‐T antigens, OKT3+, and OKT11+(erythrocyte rosetting+) was observed in most patients. Six of the seven cases tested showed la and DR antigens. No relationship was detected between patterns of reactivity with T cell reagents and histological types. When tested, the in‐vitro malignant T cells of five patients proliferated in response to concanavalin A (Con A), but had poor response to phytohaemagglutinin. The interleukin 2 receptors showed maximum expression on Con A‐activated T cells of five patients, and phytohaemagglutinin‐activated T cells of one patient. The neoplastic T cells (OKT4+, OKT8+) of one patient studied had suppressor activity for IgG and IgA, and helper activity for IgM synthesis on pokeweed mitogen‐induced normal B cell diffe

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02376.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

In vivo activated T cells could be isolated from cerebrospinal fluid (CSF) of a patient suffering from chronic meningitis of unclear origin. Although the patient's skin reactivity to purified protein derivative (PPD) was negative, and peripheral T cells did not proliferate to this antigen in vitro, the majority of T cell clones from CSF specifically recognized PPD on either autologous or allogeneic HLA class II compatible macrophages. Remarkably, peripheral blood mononuclear cells potently suppressed the PPD‐specific proliferate responses of healthy donors. The selective enrichment of oligoclonal IgG in the CSF but not in the patient's serum further indicated T and B cell responses lacking systemic feedback control. Analyses of a persisting immune stimulation in the CSF provide a potent diagnostic tool and may explain neurological complications as observed in a number of autoimmune diseases and chronic infection

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02377.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Protective immunity against mycobacteria is dependent on antigen‐specific T cells. The antibodies induced upon immunization with mycobacteria have no apparent role in host protection. Serological techniques have detected some antigens that are also recognized by human T cells but may fail to recognize others. Potentially, there may he differences in the epitopes seen by the T and B cell anti‐mycobacterial antigen repertoires‐ We have screened the different components of sonicated BCG orMycobacterium lepraethat were separated according to their molecular weight (MW) by SDS‐PAGEI and then electroblotted on nitrocellulose paper. The blots were cut into squares and tested directly in a T cell proliferation assay. Our results indicate that peripheral T cells of healthy leprosy patient contacts respond preferentially to the tower MW (<70,000) and not the higher MW fractions ofM. lepraeand BCG, in contrast to the humoral response of these same individuals. The most important fractions in inducing a lymphoproliferative response were in the regions of 11–16kDa of BCG andM. lepraeand to the 22–26kDa region ofM. leprae.These fractions appeared to represent molecular weight regions that were in some instances clearly distinct from previously defined antigens. It was further shown that lymphoproliferation in response to mycobacterial fractions correlated with the production of gamma interferon, a lymphokine required for macrophage activation and elimination of mycobacteria. These studies allow the direct assessment of antigens involved in protective T cell‐mediated immunity, and should be helpful in selecting relevant antigens for skin testing and

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02378.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Histamine inhibited the production of interleukin 1 (IL‐1) induced by lipopolysaccharide (LPS) in cultures of purified human peripheral blood monocytes. The effect of histamine on IL‐1 production was dose‐dependent and significant at histamine concentrations of 10−4‐10−5m. The histamine H2 receptor agonists dimaprit and 4‐methylhisiamine, hut not the H1 receptor agonists 2‐pyridylethylamine. aminoethylthiazole and 2‐methylhistamine, modulated the IL‐1 production in a similar manner to histamine. The inhibitory effects of histamine could be reversed by the H2 receptor antagonist cimetidine but not by the H1 receptor antagonist mepyramine. This indicates that the inhibitory effects of histamine on LPS‐induced IL‐1 production are mediated through H2 receptors on human per

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02379.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

In the context of ex vivo T cell elimination from bone marrow, the anti‐T cell cytotoxic potential of immunotoxins (IT) prepared by conjugation of die monoclonal antibodies (MOAb) WT32 (CD3), T101 (CD5), and WT1 (CD7) to ricin A chaw was evaluated. The cytotoxicity of IT was based on protein synthesis inhibition in human T cell lines: GHI, CEM, HPB‐ALL, and Jurkat, and appeared closely related to the antigen density and internalization rate of the IT. Normal unstimulated T cells appeared to he rather insensitive to IT not due to a low antigen density or decreased internalization. The cytotoxicity of IT to T cells could he enhanced considerably by NH4Cl. Treatment of T cells with a cocktail of IT (10−8m) and 20 mmNH4Cl resulted in a 5000‐fold cytoreduction as measured by clonogenic assays of limiting T cell dilutions, whereas the haematopoietic progenitor cells remained unaltered. Stimulation of T cells with phytobaemag‐glutinin (PHA) prior to incubation with IT considerably increased the sensitivity to IT treatment. Thus, normal T cells are less sensitive to anti‐T cell IT than T cell lines and activated T cells. This suggests that a low protein synthesis is responsible for the resistance to IT. However, a high specific cytotoxicity of IT to normal T cells can be achieved in the presence of 20 mmammoni

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02380.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Three monoclonal antibodies (MoAb), TH97A, CC13, and CC14, define a thymic differentiation antigen in cattle. The antigen is expressed on 50–60% of bovine thymocytes, located mainly in the cortical areas, but is not expressed on peripheral blood mononuclear cells (PBMC). In cryostat sections of lymph node, the antibodies read with large dendritic‐like cells in the paracortical regions. They also react with a proportion of the large ‘frilly’ cells in afferent lymph and with dendritic‐like cells in the dermis. The antibodies apparently do not react with cells in the epidermis. Biochemical analysis of the antigen recognized by MoAb TH97A reveals two bands of 44 kDa and 12 kDa under reducing conditions. These polypeptides are distinct from bovine class I major histocompatibility complex molecules reactive with the MoAb w6/32. The tissue distribution of positive cells together with results of biochemical analyses indicate that the antigen recognized by these MoAb is the bovine analogue of the

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02381.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The relationship between polyclonal B cell activation and immunosuppressor effects induced by F5'EP‐Sm, a non‐cytotoxic protein secreted byStreptococcus mutans, was studied in C57BL/6 mice. Mice created with F5'EP‐Sm exhibited a considerable increase in splenic nonspecific Ig plaque‐forming cells (PFC) compared with untreated mice. The isotypic pattern of non‐specific PFC responses favours IgG2a∼IgG2b>IgG3>IgG1∼IgM, when taken as a ratio between treated and untreated animals. When F5'EP‐Sm was administered 2 days before immunization with sheep red blood cells (SRBC), the non‐specific PFC production was accompanied by an ephemeral increase in specific PFC against SRBC 1 day after immunization, which was quickly replaced by a strong immunosuppression. In contrast, when F5'EP‐Sm was injected after priming, there was little or no demonstrable suppression of specific PFC, and the increase of non‐specific PFC was much less evident. The kinetic curves representing increase or decrease in relation to controls of specific and non‐specific PFC are almost mirror images in each of the isotypes. The in vivo suppressor effect was abrogated in thymectomized mice, although the involvement of the T cell compartment is probably secondary to the B cell mitogen effect, since T‐depleted spleen cells proliferate and synthesize non‐specific Ig when stimul

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02382.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The ability of different subpopulations of blood mononuclear cells to serve as accessory cells in the activation of CD4+and CD8+T cells via Ti‐CD3 or with phytohaemagglutinin (PHA) was studied. Pure CD4+or CD8+T cells did not respond to particle‐bound anii‐CD3 monoclonal antibodies (MoAb) or PHA, whereas responses were seen when non‐T cells served as accessory cells. Removal of class II‐positive cells from peripheral blood mononuclear cells (PBMC) or from non‐T cells diminished, but did not completely abolish, the responses in both T cell subsets, indicating that the accessory cells are mainly found among the class II‐positive cells. However, the class II molecules themselves were not involved, as demonstrated in antibody‐blocking experiments. Removal of monocytes decreased the ability of non‐T cells to serve as accessory cells for both CD4+and CD8+cells in PHA activation. In contrast, the removal of monocytes resulted in an enhanced activation by anti‐CD3 MoAb in CD4+T cells, while the activation of CD8+T cells was less affected. Positively selected B cells were effective accessory cells in anti‐CD3 and PHA activation. Furthermore, Epstein‐Barr virus (EBV)‐transformed B cell lines were very potent accessory cells both in anti‐CD3 and PHA activation of T cells, and showed the strongest accessory cell function observed in this

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02383.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

We have utilized a new method for obtaining highly purified cells using positive selection by immunomagnetic separation to study the conditions required for phytohaemagglutinin (PHA) activation of pure T4 and T8 cells. In the presence of accessory cells (AC), a comparable proliferative response was obtained in the two subsets. In the absence of AC, PHA induced low levels of interleukin 2 (IL‐2) receptor expression as well as responsiveness to IL‐2 in both T4 and T8 cells. If AC or 12‐O‐tetradecanoyl‐phorbol‐13‐acctate (TPA) were also present, IL‐2 production and DNA synthesis were seen in both subsets. A short preincubation with PHA primed' T fells for subsequent responsiveness to IL‐2 or TPA, while preincubation with TPA did not induce response to PHA. Thus, PHA alone is sufficient for the first step of T cell activation lending to IL‐2 receptor expression. The second step leading to IL‐2 production, is dependent on direct interaction with AC in the presence of PHA. While T8 cells are dependent on help by T4 cells for proliferation to occur during stimulation with antigen, in PHA stimulation the requirements for activation and proliferation seem to he identica

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02384.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY