摘要:

We observed that highly purified E‐rosette‐negative largely leukaemic B cells from 9 out of 15 patients with chronic lymphocytic leukaemia (CLL) significantly suppressed immunoglobulin production by mixtures of T4 and B cells from normal donors in the presence of pokeweed mitogen (PWM). This suppression by leukaemic B cells was concentration‐dependent. Addition of equal numbers of B cells from normal donor to the mixtures of normal T4 and B cells increased, or had no effect on the production of IgM, IgA, and IgG Treatment of purified largely leukaemic B cells from patterns with CLL with either the anti‐B1 or anti‐Leu 1 monoclonal antibody plus complement abolished their ability to suppress immunoglobulin production. In contrast, treatment with either the anti‐Leu 5 or the OKMI monoclonal antibody plus complement had no effect on the suppression. These results tugged that leukaemic B cells from certain patients with CLL may exhibit, or can be induced to exhibit, immunosuppressive

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02409.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Peripheral blood and bone marrow mononuclear cells from 25 children with acute non‐lymphoid leukaemia were analysed for natural killer cell activity and for cells with the Leu‐7 and Leu‐11b(CD 16) markers. Significantly reduced spontaneous cytotoxicity was detected in peripheral blood from children with untreated and active acute non‐lymphoid leukaemia compared with that of the controls (P=0.01 andP<0.05). Patients to remission, however, had normal natural cytotoxicity and normal number of Leu‐7 and Leu‐11b(CD 16)‐positive cells. The natural killer cell activity in hone marrow from patients with untreated acute non‐lymphoid leukaemia was also significantly reduced (P=0.025). On die other hand, patients in remission had both an increased percentage of Leu‐7 and Leu‐11b (CD 16)‐positive cells (P<0.05) and an increased natural killer cell activity (P<0.0005) in their bone marrow cells in comparison with the control group. This augmented natural killer cell activity is most probably a result of anti‐leukaemic treatment. Stimulation with recombinant alpha interferon and recombinant interleukin 2 caused an increase in natural killer cell activity that was both significant and normal in both peripheral blood and bone marrow from children with acute

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02410.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

We have previously shown that various benign and malignant natural killer (NK)‐resistant monolayer cells inhibit endogenous human NK activity, probably by reducing the secretion of cytotoxic factors from the effector cells. The nature of she molecules responsible for the inhibition has been unclear. In this study we show that phosphate‐buffered saline (PBS) extracts of ovarian cystadenocarcinoma tissue and normal uterine smooth muscle strongly inhibit NK activity. Fractionation of tumour extracts by gel chromatography revealed major inhibitory activity in theMrrange 160,000–180,000, and other weaker Inhibiting activities in theMrranges 50,000–70,000 and 20,000. The active material ofMrrange 160,000–180,000 was adsorbed on anion exchange chromatography column at neutral pH and physiologic NaCl concentration, and it was eluted by 0.31–0.34mNaCl. The inhibitory molecule was sensitive to proteolysis. No relation of this compound to immunoglobulins or trypsin and urokinase inhibitors was detected. The unfractionated extract inhibited NK activity apparently by the same mechanism as the monolayer target cells, i.e. by reducing the secretory capacity of effector cells. The data strongly suggest that the NK‐inhibiting compounds described in this work lire involved in the inactivation of NK cells by intact m

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02411.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Nine hybridomas producing monoclonal autoantibodies specific for kidney proximal tubular brush border were Found in 600 hybridomas derived from (C57BL/6J×DBA/2)F1mice injected with DBA/2 T cells. None of the 1100 hybridomas derived from nonautoimmane (C57BL/6J×DBA/2)F1mice produced antibodies with a similar specificity. Four of these nine monoclonal antibodies were characterized further. They did not bind to cryosections of liver, lung, stomach, or intestine. Three bound to kidney proximal tubular brush border of mouse, cattle, sheep, pigs, rabbits, rats, and humans, whereas the fourth was specific only for murine brush border. All four precipitated from mouse kidney microvilli, a protein with an apparent molecular weight of 160,000 under reducing as well as nonreducing conditions. Removal of asparagine‐linked carbohydrate with EndoF reduced the molecular weight of the 160,000 protein by about 20,000. One of the three multi‐species‐specific antibodies bound to pig kidney aminopeptidase, a glycosylated enzyme located on the microvilli of kidney proximal tubular brush border. Three antibodies have a heavy‐chain variable region encoded by VHgenes of the J558 family, whereas the heavy‐chain variable region of the fourth is encoded by a VHgene of the 7183 family. Attempts to passively transfer immune complex glomerulonephritis to normal mice by injection of the purified monoclonal antibodies or by growth of the corresponding hybridoma cells in mice have so far been unsuccessful. However, antibodies recognizing the 160,000 molecule are present in the serum of mice with chronic graft‐versus‐host disease and can be eluted from kidneys with immune complex glo

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02412.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The dynamics of cytotoxic T lymphocyte precursors (CTL‐p) in mice injected with allogeneic spleen cells (SC) was studied with special reference to changes in their radiation sensitivity. Whole‐body 400 rad X‐ray irradiation of allo‐SC‐primed and unprimed mice virtually abolished the capacity of their SC to proliferate and to generate CTL in primary or secondary mixed leucocyte culture (MLC). However, the impaired ability of SC to generate CTL in the primary MLC was restored by interleukin 2 (IL‐2). This showed that helper cells whose activity was replaceable with IL‐2 (IL‐2‐producing cells) were functionally more radiation‐sensitive than CTL‐p in unprimed mice. In contrast, the radiation‐impaired activity in secondary MLC was not restored by IL‐2, suggesting that memory CTL‐p in allo‐SC‐primed mice were unexpectedly sensitive to radiation. The D37values determined from the percentage of residual CTL‐p activity of SC in bulk cultures 1 day after irradiation were 525 rad for virgin CTL‐p and 75 rad for memory CTL‐p. Further studies demonstrated that the radiation‐sensitive memory CTL‐p were generated from relatively radiation‐resistant precursors, largely independent of radiation‐sensitive IL‐2‐producing cells and of cellular proliferation. The mean frequency of CTL‐p in SC measured by limiting dilution assay was not significantly increased by the priming. This supports our conclusion that the development of the memory CTL‐p activity in allo‐SC‐primed mice did not depend on clonal expansion. Whole‐body 400 rad‐irradiation reduced the frequency of CTL‐p in SC from unprimed‐mice 1/2–1/3 and that in SC from allo‐SC‐primed mice to 1/8–1/15. This supports the view that the majority of radiation‐resistant virgin CTL‐p functionally mature to radiation

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02413.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

By means of a panel of monoclonal antibodies it is demonstrated that, in cultures of human peripheral blood mononuclear cells (PBMC) with the T‐cell dependent (TD) antigen ovalbumin (OA), responding B ceils are activated from the resting Mate The differentiation of the activated B cells to high rate‐secreting plasma blasts, however, is attested in an early activation phase, in which they can be detected at low rate‐secreting plaque‐forming cell. The arrest does not occur when stimulation with OA occurs in the presence of antigen‐nonspecific activation and maturation factors, which are provided in the culture by the anamnestic response to the TD antigen tetan

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02414.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The marine T‐cell line 2.19, orginally used for cloning of interleukin 4 (IL‐4) and IL‐5, was analysed for surface marker characteristics using monoclonal antibodies and a FACS‐4analyser and found to be positive for the B220 antigen. Thus, this lymphokine‐producing cell with charactistic T‐cell markers expresses an antigen earlier thought to be specific for B cells. With two‐colour fluorescence it was found that a small fraction (2–4%) of Thyl+spleen cells from normal CBA mice were also po

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02415.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Receptors encoded by the Vβ8gene family detected by the monoclonal antibody F23.1 are expressed among ‘naturally’ activated T cells in normal spleen at frequencies significantly higher than in the total CD4+and CD8+cell populations. The positive selection of these clones into ‘natural’ T‐cell activity could be the reason for the high frequencies of cells expressing Vβ8genes. This phenotype is strai

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02416.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The role of CD8 (Lyt‐2) in the function of a long‐term cytotoxic T‐cell (CTL) clone (C196) was analysed. Previous studies had shown that C196 cells utilize the αβT‐cell receptor and depend on Lyt‐2 to recognize and lyse P815 mastocytoma target cells. Recognition is H‐2Kdrestricted, presumably involving a P815 specific antigenic structure. Here we analyse a number of variants selected from C196 that have reduced or virtually abolished expression of Lyt‐2, alone or in combination with other deficiencies. We studied the ability of these variant cells to lyse either P185, a process requiring both specific antigen recognition and triggering of cytolytic function, or to lyse the anti‐CD3 hybridoma 145‐2C11, a process that requires the triggering of cytolytic function only. The results shows that in the case or a permanently activated CTL, effector cell such as C196, Lyt‐2 is required for antigen recognition but is not essential for the trigg

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02417.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Influenza nucleoprotein (NP)‐specific cytotoxic T lymphocytes (CTL) stimulated by immunization of mice with VV‐PR8‐NP6, a recombinant vaccinia virus expressing A/PR/8/34 NP, did not protect mice against challenge with A/PR/8/34 4 days later. Neither were secondary NP‐specific CTL stimulated by reimmunization able to protect mice. These results contrast with the ability of transferred, in vitro‐cultured and stimulated, NP‐specific CTL to protect recipient mice from challenge with A/PR/8/34. Immunization of mice with a recombinant vaccinia virus expressing A/PR/8/34 HA protected mice challenged 4 days later, either via the small amount of antibody already present, or via HA‐specific CTL that would have to be more efficient than NP‐specific CTL in either trafficking to the infected lung or in ef

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1988.tb02418.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY