摘要:

The relative distribution of IgA and IgG subclass‐producing immunocytes was examined by two‐colour immunohistochemistry in normal human distal ileum including Rover's patches (PP). regional mesenteric lymph nodes (MLN). and peripheral lymph nodes. IgA2 cells predominated slightly over IgA1 cells in the PP dome area. There was a decreasing median proportion of IgA2 cells in the order of PP (52%), distant ileal lamina propria (40%), MLN (32%), and peripheral lymph nodes (11%). The reverse was (rue for IgA1 cells in independent enumerations. These results support the notion that PP‐derived B cells after stimulation are seeded mainly to the lamina propria of the distal gut, but that there is a substantial retention and terminal differentiation of this migrating population in regional MLN. The median subclass proportions of IgG‐producing cells in the PP dome area were in independent determinations 68% IgG1.23%. IgG2, 8% IgG3, and 9% IgG4. This distribution was fairly similar to that seen in other tissue categories, except for a trend towards increased IgG1 and reduced IgG2 proportions in peripheral lymph nodes and reduced IgG1 along with increased IgG3 in normal palatine tonsils. The data suggested an association between the expression of IgG2 (and possibly IgG4) and IgA2 in intestinal mucosal immune re

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02894.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Previous work has shown that splenic macrophages derived from herpes simplex virus (HSV)‐resistant C57BL/6 mice undergo a persistent HSV infection which is characterized by the continuous release of infectious virus particles from a small subpopulation of infected cells. Treatment of persistently infected macrophages for 2 weeks with lipopolysaccharide (LPS) resulted in an increase of HSV yield and in virus‐induced cytopathic effects. HSV was also reactivated by treatment of macrophage cultures with lipid A or tumour necrosis factor (TNF). Like macrophages of C57 BL/6 origin, cells from LPS‐hyporesponsive C3H/HeJ mice could be persistently infected with HSV. These cells were resistant to LPS‐induced virus reactivation. The results show that macrophages derived from C57BL6 mice are rendered susceptible to lytic HSV infection by treatment with LPS or TNF. Thus, these substances may interfere with persistent HSV infection which can he established due to genetically controlled properties of t

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02895.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Immunoprecipitation of radioiodinated hairy cell leukaemia (HCL) cell lysates with monoclonal antibody (MoAb) HML‐1. originally reported to recognize intraepithelial T cells, and with MoAb B‐ly7, originally reported to react with HCL. led to identical biochemical characteristics. In SDS PAGE under reducing conditions, a major band of 143 kDa. a broad band ranging from 112 to 122 kDa. and two additional faint bands of 175 and 100 kDa could be determined. Deglycosylation of N‐linked sugar moieties by treatment of immunoprecipitates with endoglycosidases indicated that the two main protein cores of the antigen are predominantly if not exclusively glycosylated by complex and hybrid types of oligosaccharide chains. Competitive binding inhibition demonstrated that both MoAb are directed against different epitopes. Immunohistochemically. the staining patterns obtained with both MoAb in normal tissues, in T‐and B‐cell lymphomas, and in HCL were identical except for a single case of HCL which was HML‐1−/B‐ly‐7+, We conclude that MoAb HML‐1 and B‐1y7 recog

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02896.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Thirty‐one patients with lepromatous leprosy received recombinant interleukin 2 (IL‐2) intradermally in doses ranging from 10 to 30 μg. Before injection and at time intervals of 2–21 days thereafter, samples of peripheral blood mononuclear cells (PBMC) were obtained. Single or multiple injections (1–3) of IL‐2 did not modify the total number of circulating lymphocytes or the number of T cells and the CD4/CD8 T‐cell ratio However. IL‐2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4–8 days alter intradermal IL‐2. Stimulation indices of three‐ to sevenfold above pre‐IL‐2 levels were observed with the polyclonal activator phytohaemagglutinin (PHA) and enhanced thymidine incorporation occurred in the presence of antigens to which the patients were already sensitized, such as purified protein derivative and BCG. IL‐2 had no effect on the unresponsive stale of lepromatous leprosy patient T cells to the antig

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02897.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The process of graft‐versus‐host disease (GVHD) elicited by small bowel semi‐syngeneic gratis in Lewis rats was studied by an immunohistochemical staining technique for analysis of MHC (major histocompatibility complex) class II antigen expression and of T‐cell subpopulations in different organs. Specimens from the graft, native bowel, brain, testis, liver, kidney, and skin were taken on days 5,10, and 15. All the investigated organs displayed strong class II antigen induction during the course of GVHD. In the native bowel of semi‐syngcneically transplanted animals, only discrete morphological changes were noted, whereas the graft displayed a generalized serosal reaction with large infiltrates of rounded and polygonal cells expressing class II antigens. This was not observed in the graft of syngeneically transplanted animals. In the lamina propria of the semisyngeneic graft,‘free’lymphocyte‐like cells were depleted and, at the same time, localized aggregates of these cells were observed. Crypt cell class II expression in the native bowel, and to some extent in the graft, was increased during GVHD. However, pronounced intraindividual variations in MHC class II antigen expression were noted, and class II expression was therefore not considered to be a good

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02898.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A mouse model forL. aethiopicainfection is described. BALB/c mice were unable to clear an infection with 1 × 107promastigotes injected into the hind footpad. However, there was no ulceration of the lesion and no development of overt clinical symptoms after 203 days of infection. Spread of viable organisms was evident in the draining lymph node but not in the spleen or liver. The control of the infection was associated with the development of classical delayed hypersensitivity responses to phenolized promastigotes and appeared as a localized granulomatous infiltration. The infiltration had features of classical tuberculoid granulomas, but superimposed on it was a strong eosinophilic infiltration. The relevance of such cells though unclear is discussed

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02899.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

This study investigated the phenotype and function of different antigen‐presenting cells (APC) present within the epidermis of patients with cutaneous T‐cell lymphoma (CTCL). Involved epidermis of CTCL compared with uninvolved was found to contain increased numbers of CDI+DR+APC. This population was heterogeneous and comprised both leucocytes of a novel CD1+DR+CD36 (OK.M5)+phenotype and CD1+DR+CD36−indeterminate/Langerhans cells. The CD1+DR+CD36+leucocytes did not express TcR‐1, CD5, CD 15. or CD22, and only a minor population expressed CD11, demonslrating that they were neither T nor B cells, and did not belong lo the major CD11+(OKM1+) blood monocyte population. Electron microscopy of purified CD36+lesional epidermal cells (EC) demonstrated that they lacked Birbeck granules found on CD1+‐selected Langerhans cells, and most cells exhibited features of indeterminate cells or macrophages.The capacity of EC from involved epidermis to present alloanttgens was found to be increased relative to uninvolved epidermis in all patients tested, and this capacity was critically dependent upon the presence of CD45+DR+bone marrow‐derived cells but not on the presence of CD45+DR+keratinocytes. Positive selection using MoAb against CDI and CD36 demonstrated that both cell populations exhibited the capacity to stimulate T cells. The results indicate that a novel antigen‐presenting cell population with a unique phenotype is present within involved skin of patients with mycosis fungoides. These cells express CD36 in addition to CD1 and have an ultrastructural appearance consistent with a dendritic antigen‐presenting

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02900.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

C6 is a potentially therapeutic murine monoclonal antibody that recognizes the class 1 outer membrane protein ofNeisseria meningitidis.C6 specifically immunoblots this antigen and augments in vitro killing ofN. meningitidisbacteria. We describe a general method of obtaining the heavy and light chain variable‐region sequence from immunoglobulin‐secreting cells, The method uses mixed polymerase chain reaction (PCR) primers designed from the 5 end of the framework 1 (FR1) sequences of the heavy and light chains, and 3‐end primers for constant‐region conserved sequences. The method has been applied to the cloning and sequencing of the variable region of C6 to construct a humanized monoclonal antibody. Rapid amplification and sequencing of variable regions by this general method have multiple applications in the study of the immune response to infectious d

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02901.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Peripheral blood mononuclear cells were enriched for γδ T cells by immunomagnetic separation, stimulated with cells from an allogeneic donor, and cloned. T‐lymphocyte clones (TLC) of the two major γδ T‐cell subsets, BB3−(i.e. Vδ2+)and δTCSI+(i.e. Vδ1/(D)/Jδ1). were obtained. In addition, one γδ TLC was BB3−δTCSI+. All of the BB3+TLC showed strong cytotoxicity against various allogeneic tumour cell lines, such as Daudi and K562. The cytotoxicity against the tumour cell lines was modulated by MoAb against the γδ TcR. The BB3+TLC were not cytotoxic against B‐lymphoblastoid cell lines (B‐LCL). In contrast, the δTCSI+TLC showed much lower cytotoxic activity against the tumour cell lines, but many were strongly cytotoxic against allogeneic B‐LCL. Some of the δTCSI+TLC demonstrated HLA‐specific cytotoxicity, while other δTCSI+TLC had a more broad cytolytic activity against B‐LCL. Thus, the two major subtypes of γδ T cells from this donor, as defined by MoAb BB3 and δTCSI, were distinct wit

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02902.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We have previously shown [10] that large granular lymphocytes (LGL) are inactivated by contact with natural killer (NK) resistant monolayer target cells. In this work we have analysed which adhesion molecules are involved in the binding of LGL to such targets, as exemplified by fibroblasts, and in the subsequent inhibition of their NK activity. The result‐, indicate that antibodies against CD54 (intercellular adhesion molecule 1. ICAM‐1). CD11a (leucocyte function antigen I, LFA‐l, α chain), and CD18 (common β chain of the β2‐integrin family) significantly (by 50%) reduce the binding of LGL onto inhibitory target cells. The matrix protein‐based synthetic peptide RGD (arginine‐glycine aspartic acid. Ref. 19) and attli‐CD29 (the common β chain of the β1integrin family) antibodies also diminish the binding (by 35%). The effects of the antiadhesion molecule antibodies and the peptide are additive, the combination of both leading to an almost complete block of adhesion. It may be hypothesized that sonic of the binding‐relevant adhesion molecules of the RGD‐binding domain on LGL (CD29) may be involved in the delivery of the inactivating signal to the effector cell. Indeed, incubation of LGL with anti‐CD11a antibodies, but neither with antibodies against other binding‐relevant epitopes nor with RGD. significantly reduced their NK. activity. The mechanism of the inactivation was similar to that induced by intact NK‐resistant target cells [7]. On the basis of the present results we suggest that the CD11a molecule is involved in the down‐regulation of the NK activity o

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1990.tb02903.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY