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11. |
β-Adrenergic Stimulation of Adenosine-3’,5’-Monophosphate (c-AMP) in Primary Cultures of Rat Anterior Pituitary Cell Populations Separated by Unit Gravity Sedimentation |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 78-83
Luc Swennen,
Myriam Baes,
Carla Schramme,
Carl Denef,
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摘要:
The effect of the β-adrenergic agonist isoproterenol (ISO) on c-AMP accumulation and on growth hormone (GH) and prolactin (PRL) release was studied in primary cultures of anterior pituitary cell populations with different proportional number of somatotrophs and lactotrophs, obtained by velocity sedimentation at unit gravity. ISO stimulated c-AMP levels in highly-enriched lactotroph (∼7O%) as well as in highly-enriched somatotroph (∼65%) populations. ISO-stimulated c-AMP accumulation was attenuated by dopamine (DA) in highly enriched lactotroph but not somatotroph populations. In a small-cell population consisting of a much lower proportional number of lactotrophs and somatotrophs, the proportional increase of c-AMP accumulation was several times higher than in the other populations and the effect was not suppressible by DA. In this small-cell population, GH but not PRL release was more responsive to ISO than in the other populations. The present observations are consistent with the interpretation that the changes in c-AMP levels induced by ISO originate in part in somatotrophs and lactotrophs. However, the present data also demonstrate that the most responsive cell population in terms of c-AMP accumulation consists of small cells. The majority of these cells could not be identified. The possibility that these cells may be nonsecreting folliculostellate cells is discu
ISSN:0028-3835
DOI:10.1159/000124055
出版商:S. Karger AG
年代:1985
数据来源: Karger
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12. |
Active Clearance of Corticotropin-Releasing Factor from the Cerebrospinal Fluid |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 84-87
Edward H. Oldfield,
Heinrich M Schulte,
George P Chrousos,
Jack P Rock,
Paul L Kornblith,
Dondra L O’Neill,
David G Poplack,
Philip W Gold,
Gordon B. Cutler, Jr.,
Lynn Loriaux,
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摘要:
Intracerebroventricular injection of corticotropin-releasing factor (CRF) in animals activates behavioral, motor, metabolic and sympathetic responses similar to those seen with stress. To explore the pharmacokinetics of this peptide in the primate ventricular CSF, we determined the clearance of ovine 125I-CRF from the CSF and compared it to that of 111In-DTPA, a substance cleared by bulk flow. 125I-CRF was cleared from the ventricular fluid sixfold more rapidly than bulk flow. This suggests that the CSF may be a pathway for physiological distribution of this releasing factor to active sites within the central nervous system, and that a mechanism exists for active removal of CRF from the ventricular CSF.
ISSN:0028-3835
DOI:10.1159/000124056
出版商:S. Karger AG
年代:1985
数据来源: Karger
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13. |
Stimulation of Growth Hormone Release by Vasoactive Intestinal Peptide and Peptide PHI in Rat Anterior Pituitary Reaggregates |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 88-91
Carl Denef,
Carla Schramme,
Myriam Baes,
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摘要:
In superfused rat anterior pituitary cell reaggregates, cultured for 5 days in serum-free defined medium, vasoactive intestinal peptide (VIP) concentration-dependently stimulated prolactin (Prl) release but had only a marginal effect on growth hormone (GH) release. When reaggregates were cultured in the presence of 80 nM dexamethasone (Dex) VIP strongly stimulated GH release from a concentration as low as 0.1 nM VIP did not stimulate LH release. Peptide PHI also stimulated GH release but thyrotropin-releasing hormone (TRH) or angiotensin II did not. In fact, TRH slightly but transiently inhibited basal GH release and strongly inhibited VIP-stimulated GH release. GH-releasing factor (GRF) stimulated GH more potently and with higher intrinsic activity than VIP but GRF did not increase Prl release. The present data indicate that under defined hormonal conditions VIP and PHI are capable of stimulating GH release and that TRH can antagonize this effect by a direct action on the pituitary.
ISSN:0028-3835
DOI:10.1159/000124057
出版商:S. Karger AG
年代:1985
数据来源: Karger
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14. |
Evidence for the Existence of Atrial Natriuretic Factor-Containing Neurons in the Rat Brain |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 92-94
David M. Jacobowitz,
Gerhard Skofitsch,
Harry R. Keiser,
Robert L. Eskay,
Nadav Zamir,
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摘要:
Immunoreactive atrial natriuretic factor- (ANF-)positive nerve fibers and cell bodies were observed in the preoptic area, hypothalamus, mesencephalon, and pons of rats. In colchicine-treated animals a large number of immunoreactive ANF-positive cell bodies were seen in the organum vasculosum of the lamina terminalis, in several hypothalamic nuclei (e.g. periventricular, arcuate, and ventral premammillary nuclei), and in the dorsolateral tegmental nuclei of the pons. Varicose nerve fibers containing ANF were generally observed in the vicinity of the cells. These findings indicate that a widespread network of ANF-containing neurons is present in the brain.
ISSN:0028-3835
DOI:10.1159/000124058
出版商:S. Karger AG
年代:1985
数据来源: Karger
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15. |
Report from the International Society of Endocrinology |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 95-95
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PDF (128KB)
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ISSN:0028-3835
DOI:10.1159/000124059
出版商:S. Karger AG
年代:1985
数据来源: Karger
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16. |
Announcements |
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Neuroendocrinology,
Volume 40,
Issue 1,
1985,
Page 96-96
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PDF (144KB)
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ISSN:0028-3835
DOI:10.1159/000124060
出版商:S. Karger AG
年代:1985
数据来源: Karger
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