|
|
| 11. |
Announcement |
| |
Neuroendocrinology,
Volume 64,
Issue 1,
1996,
Page 69-69
Preview
|
PDF (182KB)
|
|
ISSN:0028-3835
DOI:10.1159/000127100
出版商:S. Karger AG
年代:1996
数据来源: Karger
|
| 12. |
17β-Hydroxysteroid Dehydrogenase Activity Correlates with the Type-2 17β-Hydroxysteroid Dehydrogenase mRNA Abundance in Human Meningioma Tumors |
| |
Neuroendocrinology,
Volume 64,
Issue 1,
1996,
Page 70-78
Jean-Louis Carsol,
Jerzy Adamski,
Olivier Guirou,
Henriette Gérard,
Pierre-Marie Martin,
Yvan de Launoit,
Preview
|
PDF (1759KB)
|
|
摘要:
Benign meningioma tumors possess significant levels of 17β-hydroxysteroid dehydrogenase (17β-HSD) activity. Two different 17β-HSDs were discovered in human placenta: one highly estrogen specific and using NADP-VNADPH as cofactors (type-1 17β-HSD), and a second one that utilizes both androgens and estrogens as substrates with NAD+/NADH (type-2 17β-HSD). Recently, two further human 17β-HSDs were isolated. A testis-specific 17β-HSD (type-3 Πβ-HSD) favors the reduction of △4-androstenedione to testosterone, and a ubiquitously expressed type-4 17β-HSD preferentially catalyzes the oxidation of estradiol and Δ5-androstenediol. In this study we characterize the expression levels of different types of 17β-HSD in a wide series of tumors. Using the Northern blotting method we show that type-1, -3, and -4 17β-HSDs are not detectable in meningiomas. In contrast, the type-2 17β-HSD RNA is present in 6 of 17 meningiomas and its abundance is directly correlated with estrogenic 17β-HSD activity (r2 = 0.74). The presence of type-2 17β-HSD is also demonstrated by in situ hybridization. RT-PCR and Western blots show that type-4 17β-HSD is also present, though at much lower levels. The progesterone receptor level, the epidermal growth factor receptor level, and the age of the patients are not correlated with the estrogenic 17β-HSD activity or type-2 17β-HSD m
ISSN:0028-3835
DOI:10.1159/000127101
出版商:S. Karger AG
年代:1996
数据来源: Karger
|
|
首页
