ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02765.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02766.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02768.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02769.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryAn indirect immunoperoxidase (IMP) technique was applied to cryostat and paraffin sections of liver from ten aborted equine foetuses. Equid herpesvirus type 1 (EHV‐1) had been isolated from seven of them and EHV‐4 from one: the remaining two were virologically negative and were not used as controls. In the eight virus‐infected cases the immunostaining revealed foci of cells exhibiting a distinct brown cytoplasmic and inclusion body pigmentation. No specific signal was present in the non‐infected controls. The method also was adapted for incubation in a microwave oven, which allowed the total length of the procedure to be reduced to a minimum of 35 mins for cryostat sections, without significant loss of

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02770.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryIn 1988 an outbreak of the paralytic form of Equid herpesvirus type 1 (EHV‐1) infection occurred on a stud farm and several animals died. This provided an opportunity to perform detailed pathological investigations to gain insights into the pathogenesis of this spontaneous disease. Two paretic mares, three foals, an aborted foetus and its non‐paretic dam were examined. The endotheliotropism of the virus was clearly demonstrated by the use of an indirect immunoperoxidase (IP) stain. At autopsy, evidence of viral infection was widespread in the foetus and foals, but limited or absent in the mares, probably reflecting differences in their immune status. Vascular lesions were present in the central nervous system (CNS) of the foals as well as the adults; they resulted in minimal neural lesions in the foals. Severe changes in the upper and lower respiratory tracts were a particular feature in the foals, two of which exhibited extensive vasculitis and thrombosis in the lungs. The IP technique was of great value in locating antigen‐containing cells in the CNS of one mare when virus isolation was negative. It also revealed the presence of virus in less well documented sites such as the pancreas, gut, thyroid, uveal tract and the skin of the

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02771.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses‐1 and −4 (EHV‐1 and EHV‐4) DNA within nasopharyngeal swab samples from horses. Ninety‐eight samples from suspected field cases and in‐contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV‐1 and EH

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02772.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe relationship between pedicle flap width and viable length was characterised for skin flaps of the flank in ponies. Four dorsally based, pedicle type skin flaps of 20 cm in length and 3, 6, 9 or 12 cm in width were created in a random sequence on one flank in each of 10 ponies. Flap survival length was assessed by skin texture and appearance, depilation of hair and wound healing at 14 days after surgery. There was considerable variation between animals in the viable length of flaps of the same width; however, a significant difference in the viable length of flaps of different widths was detected (P=0.002). The viable length of the 3 cm flaps was significantly different from that of the 12 cm flaps (9.69 ± 1.1 cm vs 12.97 ± 1.0 cm, mean ± se P<0.05). There was a positive correlation between flap width and viable length (r2= 0.141, P=0.017). There was no effect of flap order (cranial to caudal positioning relative to the other flaps) on viable length (P=0.286). The results of this study demonstrate a significant relationship between flap width and viable length, confirming a previously unsupported assumption that flap width and viable length are related. These findings suggest that the clinician should employ broad pedicles when using local flaps to reconstruct skin defects on the flank of the hor

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02773.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe purpose of this study was to evaluate the effect of modifying commercial reagents for the laboratory evaluation of several haemostatic parameters in normal, non‐pregnant mares. The routine coagulation screening assays, namely, the activated partial thromboplastin time (APTT) and the one‐stage prothrombin time (PT), and the specific coagulation assays for the determination of the biological activity of Factors VII, VIII:C and IX, are discus

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02774.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryVarious haemostatic analytes were systematically evaluated for four months pre‐partum and five months post partum in 14 healthy mares. The plasma fibrinogen concentration and both Factor VIII:C and von Willebrand factor activity showed gradual increases from mid‐gestation and reached maximal, or near maximal activity at parturition. These increases were paralleled by an increase in plasma fibronectin concentration, the appearance of fibrinogen degradation products, and a modest rise in antithrombin III concentration. In contrast, the activity of Factor VII and Factor IX, and the one‐stage prothrombin (PT) time and the activated partial thromboplastin (APTT) time remained relatively constant throughout the pre‐ and post parturient

ISSN:0425-1644    

DOI:10.1111/j.2042-3306.1992.tb02775.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY