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1. |
Renal Dopamine Receptor Function in Hypertension |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 187-197
Tahir Hussain,
Mustafa F. Lokhandwala,
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摘要:
Dopamine plays an important role in the regulation of renal sodium excretion. The synthesis of dopamine and the presence of dopamine receptor subtypes (D1A, D1Bas D1-likeand D2, and D3as D2-like) have been shown within the kidney. The activation of D1-likereceptors located on the proximal tubules causes inhibition of tubular sodium reabsorption by inhibiting Na,H-exchanger and Na,K-ATPase activity. The D1-likereceptors are linked to the multiple cellular signaling systems (namely, adenylyl cyclase, phospholipase C, and phospholipase A2) in the different regions of the nephron. Defective renal dopamine production and/or dopamine receptor function have been reported in human primary hypertension as well as in genetic models of animal hypertension. There may be a primary defect in D1-likereceptors and an altered signaling system in the proximal tubules that lead to reduced dopamine-mediated effects on renal sodium excretion in hypertension. Recently, it has been shown in animal models that the disruption of either D1Aor D3receptors at the gene level causes hypertension in mice. Dopamine and dopamine receptor agonists also provide therapeutic potential in treatment of various cardiovascular pathological conditions, including hypertension. However, because of the poor bioavailability of the currently available compounds, the use of D1-likeagonists is limited to the management of patients with severe hypertension when a rapid reduction of blood pressure is clinically indicated and in acute management of patients with heart failure. In conclusion, there is convincing evidence that dopamine and dopamine receptors play an important role in regulation of renal function, suggesting that a defective dopamine receptor/signaling system may contribute to the development and maintenance of hypertension. Further studies need to be directed toward establishing a direct correlation between defective dopamine receptor gene in the kidney and development of hypertension. Subsequently, it may be possible to use a therapeutic approach to correct the defect in dopamine receptor gene causing the hypertension. (Hypertension. 1998;32:187-197.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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2. |
Structural Analysis and Evaluation of the Aldosterone Synthase Gene in Hypertension |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 198-204
Eva Brand,
Nathalie Chatelain,
Paolo Mulatero,
Isabelle Fery,
Kathleen Curnow,
Xavier Jeunemaitre,
Pierre Corvol,
Leigh Pascoe,
Florent Soubrier,
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摘要:
Anomalies in either of the tightly linked genes encoding the enzymes CYP11B1 (11 beta-hydroxylase) or CYP11B2 (aldosterone synthase) can lead to important changes in arterial pressure and are responsible for several monogenically inherited forms of hypertension. Mutations in these genes or their regulatory regions could thus contribute to genetic variation in susceptibility to essential hypertension. To test this hypothesis, we performed 2 complementary studies of the CYP11B1/CYP11B2 locus in essential hypertension. After characterizing a DNA contig containing the CYP11B1 gene and mapping the gene in the Centre d'Etudes du Polymorphisme Humain reference panel of families, we performed a linkage study with 292 hypertensive sibling pairs and a highly informative microsatellite marker near CYP11B1. We also analyzed the association of 2 frequent biallelic polymorphisms of the CYP11B2 gene, 1 in the promoter at position -344 (-344C/T) and the other, a common gene conversion in intron 2, with hypertension in 380 hypertensive patients and 293 normotensive individuals. Statistical analyses did not show significant linkage of the CYP11B1 microsatellite marker to hypertension. No positive association with hypertension was found with the gene conversion in intron 2, but a positive association with hypertension was found with the -344T allele. The hypertensive and normotensive samples differed significantly in both genotype (P=0.023) and allele frequencies (P=0.010). Our data suggest a modest contribution of the CYP11B2 gene to essential hypertension. (Hypertension. 1998;32:198-204.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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3. |
Appropriate Regulation of Human Renin Gene Expression and Secretion in 45-kb Human Renin Transgenic Mice |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 205-214
Yan Yan,
Lufei Hu,
Rong Chen,
Jean E. Sealey,
John H. Laragh,
Daniel F. Catanzaro,
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摘要:
To create physiological models of the human renin-angiotensin system in transgenic animals, the component genes should be expressed in the correct tissues and cells and respond appropriately to physiological stimuli. We recently showed that mice carrying a 45-kb human renin genomic fragment, containing approximately 25 kb 5[prime]-flanking DNA and 6 kb 3[prime]-flanking DNA, express the transgene in a highly cell- and tissue-specific pattern. More importantly, in contrast to previous models, human renin in the circulating plasma of these mice is derived exclusively from the kidneys. In the present study, we tested the responses of both human and mouse renal renin expression and secretion of the 45-kb hREN transgenic mice to a variety of physiological and pharmacological stimuli. A sodium-deficient diet, angiotensin-converting enzyme inhibition, and beta1-adrenergicstimulation each increased both human and mouse plasma renin concentration significantly, whereas elevated blood pressure and/or increased plasma angiotensin II levels suppressed them. Human and mouse renal renin mRNA levels changed similarly but to a lesser degree. These studies demonstrate that human renin synthesis and secretion respond appropriately in 45-kb hREN mice to physiological stimuli. This most likely results from appropriate cell-specific expression of the transgene conferred by the extended transgene flanking sequences. (Hypertension. 1998;32:205-214.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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4. |
Conditioned Medium From HepG2 Cells Transfected With Human Apolipoprotein(a) Gene Stimulates Growth of Human Vascular Smooth Muscle CellsEffects of Overexpression of Human Apolipoprotein(a) Gene |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 215-222
Ryuichi Morishita,
Shingo Yamada,
Jitsuo Higaki,
Naruya Tomita,
Iwao Kida,
Motokuni Aoki,
Atsushi Moriguchi,
Shin-ichiro Hayashi,
Ikunosuke Sakurabayashi,
Yasufumi Kaneda,
Toshio Ogihara,
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摘要:
Lipoprotein(a) [Lp(a)] is well known to stimulate growth of vascular smooth muscle cells (VSMCs), resulting in atherosclerosis. Its mechanism is postulated to be decreased in active transforming growth factor (TGF)-beta. However, the exact mechanisms and cellular processing from apolipoprotein(a) [apo(a)] to Lp(a) have not yet been clarified because no cultured cells producing apo(a) are available. Therefore, it is necessary to establish apo(a)-producing cells to study the role of apo(a). We evaluated the effects of overexpression of human apo(a) gene on human aortic VSMC growth. First, we tested whether transfection of apo(a) gene into human hepatoma cells, HepG2 cells, producing human apoB resulted in the formation of Lp(a). Transfection of apo(a) gene into HepG2 cells resulted in detectable levels of Lp(a) in the medium, as assessed by ELISA and Western blot, whereas no Lp(a) was detected in the medium of HepG2 cells transfected with control vector and untransfected HepG2 cells. Expression of apo(a) mRNA was also confirmed by reverse transcription-polymerase chain reaction. In contrast, Western blotting showed a single band detected by specific anti-apo(a) antibody, but not anti-apoB antibody, in the medium of apo(a)-transfected VSMCs. These results demonstrate that Lp(a) can be formed from apo(a) on HepG2 cells, whereas transfection of apo(a) gene into VSMCs resulted in the production of apo(a) alone but not Lp(a). Next, we examined the biological effects of overexpression of apo(a) gene on growth of VSMCs and endothelial cells. Incubation of cultured medium of HepG2 cells transfected with apo(a) gene with human VSMCs or endothelial cells resulted in a significant increase in cell number compared with the conditioned medium of HepG2 cells transfected with control vector. In contrast, transfection of apo(a) gene directly into VSMCs caused no significant effect on VSMC growth. Therefore, we measured TGF-beta concentration in the conditioned medium of VSMCs. However, using ELISA, only latent but not active TGF-beta was detected in the medium of VSMCs. Moreover, addition of neutralizing anti-TGF-beta antibody did not alter VSMC growth. These results suggest that Lp(a) could stimulate growth of VSMCs via the independent mechanisms from the inhibition of TGF-beta activation. Overall, these data demonstrate that overexpression of apo(a) gene in cells producing apoB results in formation of Lp(a), resulting in a mitogenic action on human endothelial cells and VSMCs. These results provide new information to understand the mechanisms of the mitogenic action of Lp(a) and suggest the role of Lp(a) in the pathogenesis of atherosclerosis. (Hypertension. 1998;32:215-222.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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5. |
Effect of Genetic Deficiency of Angiotensinogen on the Renin-Angiotensin System |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 223-227
Kouichi Tamura,
Satoshi Umemura,
Yoichi Sumida,
Nobuo Nyui,
Shun-ichi Kobayashi,
Tomoaki Ishigami,
Minoru Kihara,
Takeshi Sugaya,
Akiyoshi Fukamizu,
Hitoshi Miyazaki,
Kazuo Murakami,
Masao Ishii,
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摘要:
This study examined expression of renin-angiotensin system (RAS) component mRNAs in angiotensinogen gene knockout (Atg-/-) mice. Wild-type (Atg+/+) and Atg-/- mice were fed a normal-salt (0.3% NaCl) or high-salt (4% NaCl) diet for 2 weeks. Angiotensinogen, renin, angiotensin-converting enzyme (ACE), angiotensin II type 1a receptor (AT1A), and angiotensin II type 2 receptor (AT2) mRNA levels were measured by Northern blot analysis. In Atg+/+ mice, activities of circulating RAS and renal angiotensinogen mRNA level were decreased by salt loading, whereas levels of renal and cardiac ACE; renal, brain, and cardiac AT1A; and brain and cardiac AT2mRNA were increased by salt loading. Although activities of circulating RAS were not detected in Atg-/- mice, salt loading increased blood pressure in Atg-/- mice. In Atg-/- mice, renal renin mRNA level was decreased by salt loading; in contrast, salt loading increased renal AT1Aand cardiac AT2mRNA levels in Atg-/- mice, and these activated levels in Atg-/- mice were higher than those in Atg+/+ mice fed the high-salt diet. Thus, expression of each component of the RAS is regulated in a tissue-specific manner that is distinct from other components of systemic and local RAS and that appears to be mediated by a mechanism other than changes in the circulating or tissue levels of angiotensin peptides. (Hypertension. 1998;32:223-227.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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6. |
Insulin and Insulin-like Growth Factor-I Cause Coronary Vasorelaxation In Vitro |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 228-234
David Hasdai,
Robert A. Rizza,
David R. Holmes,
Darcy M. Richardson,
Pinchas Cohen,
Amir Lerman,
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摘要:
Insulin and insulin-like growth factor-I (IGF-I) may play a role in the modulation of coronary artery tone, yet there are few data regarding their vasoactive effects on the coronary vascular bed. We evaluated the vasorelaxation effects of insulin and IGF-I on porcine coronary epicardial vessels in vitro and elucidated possible mechanisms. Porcine epicardial arteries were contracted with 10-7mol/L endothelin-1 and relaxed with cumulative concentrations of either insulin or IGF-I (10-12to 10-7mol/L). The above experiments were repeated in vessels without endothelium. Vessels were also incubated with the nitric oxide synthase inhibitor NG-monomethyl-L-arginine(L-NMMA; 10-4mol/L) with and without 10-3.5 mol/L L-arginine, the potassium channel blocker tetraethylammonium (TEA; 10-2mol/L), and the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-alpha]quinoxalin-1-one (ODQ; 10-5.5 mol/L); vessels were then contracted with endothelin-1 and relaxed with insulin or IGF-I. Insulin and IGF-I were also added after contraction with 60 mmol/L KCl. Insulin and IGF-I caused a similar decrease in coronary epicardial tension after contraction with endothelin-1 (relaxation of 28 +/- 4% [n=7] and 25 +/- 3% [n=8] with insulin and IGF-I, respectively; P<0.0001 for both peptides). Removal of the endothelium did not affect these responses. Incubation with L-NMMA, but not ODQ, attenuated the vasorelaxation response to insulin and IGF in vessels without endothelium. L-Arginine did not reverse this effect of L-NMMA. KCl and TEA attenuated the vasorelaxation effect of both insulin and IGF-I. Thus, both insulin and IGF-I caused non-endothelium-dependent coronary vasorelaxation in vitro, probably through a mechanism involving the activation of potassium channels. These findings suggest that insulin and IGF-I participate in the regulation of coronary vasomotor tone. (Hypertension. 1998;32:228-234.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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7. |
Angiotensin Receptor Blockade Blunts Hyperinsulinemia-Induced Hypertension in Rats |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 235-242
Te-Chao Fang,
Wann-Chu Huang,
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摘要:
The study was conducted to examine the effects of the angiotensin subtype 1 and 2 receptor antagonists (losartan and PD123319, respectively) on blood pressure (BP) and renal excretory function in chronic hyperinsulinemia-induced hypertension in rats. Hyperinsulinemia was achieved by insulin infusion (21.5 pmol/kg per minute) via osmotic minipump for 6 weeks. Losartan or PD123319 was coinfused either at the beginning or after 4 weeks of insulin infusion. The results showed that insulin infusion significantly increased the plasma insulin concentration from 259.0 +/- 22.2 to 646.5 +/- 33.0 and 713.9 +/- 26.5 pmol/L (P<0.05) by the end of the fourth and sixth weeks, respectively, after insulin infusion. There were no significant changes in plasma glucose and triglyceride concentrations. Systolic BP increased from 139 +/- 3 to 156 +/- 1 and 157 +/- 2 mm Hg (P<0.05) at the corresponding time points. Combined losartan (3.5 [micro sign]g/kg per minute) and insulin infusion prevented the rise in BP and improved insulin resistance. When hypertension had been established after 4 weeks of insulin infusion, superimposed infusion of losartan on insulin reversed the elevated BP to control levels within 1 week. In contrast, administration of PD123319 (0.5 and 10 [micro sign]g/kg per minute) failed to alter insulin-induced hypertension. Combined PD123319 with losartan did not alter the losartan-induced hypotensive effect in insulin-infused rats. There were no significant differences in water intake, urine flow, body weight gain, and sodium gain before and after antagonist administration among groups. These results indicate that angiotensin type 1 receptors play a determinant role in the pathogenesis of insulin-induced hypertension in rats. (Hypertension. 1998;32:235-242.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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8. |
Insulin Resistance and the Effect of Insulin on Blood Pressure in Essential Hypertension |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 243-248
Tim Heise,
Kai Magnusson,
Lutz Heinemann,
Peter T. Sawicki,
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摘要:
The aim of this study was to investigate the effect of 2 weeks of insulin administration on blood pressure and to simultaneously measure insulin sensitivity and insulin-induced vasodilatation in obese hypertensive patients. In a prospective, randomized, double-blind, crossover study (study 1), 23 obese, untreated, nondiabetic, hypertensive patients received either neutral protamine Hagedorn (NPH) insulin (0.3 U/kg body wt per day) or placebo subcutaneously for 2 weeks (washout period, 2 weeks). Office and 24-hour blood pressure values were measured at the beginning and end of each treatment period. In an open-label study (study 2), 8 obese hypertensive patients and 10 healthy control subjects underwent a 3-step hyperinsulinemic, euglycemic glucose clamp (step 1, 0.5; step 2, 2.5; step 3, 5.0 mU [middle dot] kg-1[middle dot] min-1[120 minutes each]). Leg blood flow (LBF) was measured by venous occlusion plethysmography. Insulin administration decreased mean +/- SD office blood pressure from 131 +/- 13 to 128 +/- 12 mm Hg (placebo, 132 +/- 13 and 132 +/- 13 mm Hg; P<0.05 between final examinations) and mean +/- SD 24-hour blood pressure by -3.3 +/- 6.9 mm Hg (placebo, +0.7 +/- 4.6 mm Hg; P<0.05). Insulin infusion increased LBF significantly in the healthy controls but not in obese insulin-resistant hypertensive subjects. Obese hypertensive patients are resistant to the effects of insulin with regard to both glucose uptake and vasodilatation. Administration of insulin exerts a small blood pressure-lowering effect in these patients. These data strongly argue against the postulated pressor action of insulin in essential hypertension. (Hypertension. 1998;32:243-248.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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9. |
Renal Denervation Prevents and Reverses Hyperinsulinemia-Induced Hypertension in Rats |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 249-254
Wann-Chu Huang,
Te-Chao Fang,
Juei-Tang Cheng,
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摘要:
Experiments were performed to evaluate the role of the renal nerves in hyperinsulinemia-induced hypertension. Male Sprague-Dawley rats were made hyperinsulinemic by insulin infusion via osmotic minipumps implanted subcutaneously (3.0 mU/kg per minute for 6 weeks). Rats with vehicle infusion served as controls. Bilateral renal denervation was performed either at the beginning of or 4 weeks after insulin infusion. The systolic blood pressure was measured by the tail-cuff method twice a week. Food and water intake and urine flow were measured daily. The results showed that sustained insulin infusion significantly increased plasma insulin concentrations from 277.7 +/- 25.8 pmol/L to 609.9 +/- 22.2 and 696.7 +/- 23.0 pmol/L by the end of weeks 4 and 6, respectively (P<0.05). Systolic blood pressure was significantly increased from 135 +/- 3 to 157 +/- 3 and 159 +/- 2 mm Hg (P<0.05) at the corresponding time points. There was a significant increase in the plasma norepinephrine concentration after insulin infusion, whereas no significant changes in plasma triglyceride and glucose concentrations, water intake, urine flow, sodium excretion, sodium gain, and body weight gain were observed. Bilateral renal denervation depleted renal norepinephrine stores and prevented the development of hyperinsulinemia-induced hypertension. After hyperinsulinemia-induced hypertension had been fully established (from 134 +/- 2 to 157 +/- 2 mm Hg), bilateral renal denervation reversed the elevated systolic blood pressure to normotensive levels within 2 weeks. Transient denervated diuresis and natriuresis were observed. These results indicate that chronic hyperinsulinemia-induced hypertension requires the presence of intact renal nerves in rats. (Hypertension. 1998;32:249-254.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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10. |
Reference Values for 24-Hour Ambulatory Blood Pressure Monitoring Based on a Prognostic CriterionThe Ohasama Study |
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Hypertension,
Volume 32,
Issue 2,
1998,
Page 255-259
Takayoshi Ohkubo,
Yutaka Imai,
Ichiro Tsuji,
Kenichi Nagai,
Sadayoshi Ito,
Hiroshi Satoh,
Shigeru Hisamichi,
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摘要:
134/79 mm Hg and <119/64 mm Hg were related to increased risks for cardiovascular and noncardiovascular mortality, respectively. This is the first report to propose reference values for 24-hour ABP based on a prognostic criterion. (Hypertension. 1998;32:255-259.)
ISSN:0194-911X
出版商:OVID
年代:1998
数据来源: OVID
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