摘要:

AbstractThe growth of our knowledge in T‐cell biology, in particular the molecular biology of the T‐cell receptor (TCR). has provided a means to molecularly characterize lymphoproliferative diseases of the skin based on the presence or absence of a clonal population of T lymphocytes. TCR gene rearrangement studies, by Southern blot analysis, have aided the investigative dermatologist in gaining insights into the pathogenesis and clonal evolution of lymphoproliferative skin diseases. In addition, the application of TCR gene rearrangement studies as a diagnostic aid in the evaluation of lymphoproliferative skin diseases has been introduced into clinical dermatology. Despite its enormous research value. TCR gene rearrangement studies presently have limited applications as an independent diagnostic tool. However, as our knowledge and experience grows and as the application of new techniques provides us with greater detection sensitivity and specificity, the diagnostic utility of TCR gene rearrangement studies will be enhan

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00009.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00010.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractFormation of subcorneal pustules characterizes skin lesions infected byStaphyloeoccus aureus. To elucidate the mechanism underlying the subcorneal pustule formation as well as that of anti‐bacterial host defence, we studied the effect of Staphylococcal protein A on the interaction between the stratum corneum (SC) and neutrophils. We found that protein A significantly promoted opsonized SC‐induced chemiluminescence (CL) in neulrophils. This was specific to SC because no enhancement was observed with opsonized zymosan. It look place even with the serum obtained from a patient with agammaglobulinemia, ruling out the possibility of the involvement of Fc‐receptors of neutrophils in this phenomenon. Microscopic observation of such SC revealed an increase in the number of neutrophils adhering to the surface of the protein A‐coated corneocytes. Ultrastructural observation showed a distinct deformation of the neutrophils adhering to the surface of thc corneocytes, suggesting that they are in an activated stale. Such an enhanced interaction between protein A‐attached SC and neutrophils seems lo play an important role in the host defence mechanism against the invading S. aureus and in the production of the characteristic pustules by the neutrophil‐mediated damage of the surrounding epide

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00011.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractThe construction of anin vitromodel allowed an investigation of the basic functions of immunocompetent cells after laser irradiation. Among low‐energy laser sources, the helium‐neon (He‐Ne) laser, with a wavelength of 632.8 nm, has often been found to produce photobiological effects including evidence of interference with immunological functions. Previous experiments revealed an influence of He‐Ne laser irradiation on concentrations of interleukin‐lα (IL‐lα. tumor necrosis factor‐α (TNF‐α, interleukin‐2 (IL‐2), and interferon‐γ (IFN‐γ) in supernatants of cultures of human peripheral blood mononuclear cells (PBMC) with increased cytokine concentrations after irradiation of 18.9 J/cm2and decreased concentrations after irradiation of 37.8 J/cm2. Now, the mechanisms involved were studied. Results showed that cytokine production of cells stimulated with phytohemagglutinin (PHA), concanavalin A (Con A), or bacterial lipopolysaccharide (LPS) was altered significantly after laser irradiation but not after stimulation with staphylococcus aureus enterotoxin B (SEB).In situhybridization of IFN‐γ mRNA producing PBMC revealed that the number of positive cells was modulated similarly. The results were identical in cultures of enriched monocytic (Mφ) or enriched T cells. Cells of the human monocytic cell line Mono Mac 6 were also influenced after LPS stimulation, whereas constitutively 1L‐2‐producing Jurkat cells were not influenced by laser irradiation at any energy density. Analysis of the IL‐2 receptor (IL‐2R) and intercellular adhesion molecule‐1 (ICAM‐I) expression in PBMC showed partial down‐regulation of both receptors at 37.8 J/cm2, but only after stimulation with PHA. Collected data demonstrated that He‐Ne laser irradiation induces functional changes of stimulated PBMC

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00012.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractUsing reverse transcriptase‐linked polymerase chain reaction, the effect of polycyclic aromatic hydrocarbons (PAHs) on IL‐lα, IL‐lβ and IL‐6 gene expression in cultured human keratinocytes was studied. Exposure to β‐naphthoflavone and benz(a)anthracene resulted in a higher copy number of IL‐lα and IL‐6 mRNA while lower level of IL‐1β mRNA was detected in these cells. These data suggest that, like ultraviolet B (UVB) radiation, ubiquitous environmental carcinogenic PAHs are potent inducers of lL‐lα and IL‐6 cytokines and, unlike UVB, they downregulate IL

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00013.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractThe role of the two main serum opsonins (IgG and C3b) in the induction of polymorphonuclear leukocyte chemiluminescence was studied in a group of psoriatic patients. Chemiluminescence was stimulated with zymosan opsonized by fresh plasma (IgG‐ and C3b‐dependent chemiluminescence) or by complement‐depleted plasma (IgG‐dependent chemiluminescence). While C3b‐dependent chemiluminescence was similar in patients with chronic or active forms of psoriasis. IgG‐dependent chemilumi‐nescence was significantly increased in patients with active disease. However, FcR‐III expression, evaluated by means of flow cytofluorimetry. was similar in the different groups of patients studied. The discrepancy between Fc‐receptor (CD 16) expression and IgG‐dependent chemilumi‐nescence is, therefore, indicative of modifications that occur in psoriatic neutrophils that do not involve FcII

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00014.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractUVA irradiation leads to photoaging including clinical features such as wrinkle formation, reduced recoil capacity and blister formation of the skin. Besides synthesis of the extracellular matrix, its regulated degradation by various matrix‐metalloproteinases (MMPs) determines the amount and the composition of the extracellular matrix within the dermis and the basement membrane of the dermo‐epidermal junction. In this study we therefore ascertained whether UV irradiation could modulate the synthesis of MMPs with substrate specifities for dermal (collagen I, III, V) and basement membrane compounds (collagen IV, VII, proteoglycans, laminin) and whether synthesis of the counteracting tissue inhibitor of metalloproteinases (TIMP‐I) was also affected. Following UVA irradiation specific mRNAs of MMPs 1, 2 and 3 were induced concomitantly up to 5‐fold compared to mock irradiated controls. In contrast, TIMP‐1 mRNA levels remained unaltered. Immunoprecipitation indicated that after UVA irradiation synthesis and secretion of MMPs 1, 2 and 3 into the supernatant increased. Taken together, our data show that UVA irradiation coordinately induced MMPs 1, 2 and 3 implying similar mechanisms in their regulatory pathways, while TIMP‐I synthesis was not altered. Hence, unbalanced synthesis of MMPs potentially contributes to the dissolution of dermal and basement membrane compounds finally leading to blister formation and cutaneous

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1993.tb00015.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY