摘要:

AbstractFive actin genes have been identified in the mosquitoAnopheles gambiae, and a constitutively expressed actin gene has been chosen for detailed analysis. We have physically mapped and sequenced this gene and six associated cDNAs, including translated coding regions, as well as the 5 and 3 flanking sequences. Analysis of stage‐specific RNA shows this gene to be present in all stages of mosquito development and in an establishedA. gambiaecell line, thus indicating a cytoskeietal actin. In the sequence of the translated coding region and in pattern of expression, this gene is very similar to the cytoskeietal actin genes ofDroso‐phila melanogaster, and in sequence, equally similar to theArtemiacytoskeietal actin gene 403 (99.2% identity among the three amino acid sequences). Sequencing of thisA. gambiaeactin gene (designatedactWiorits location in chromosome division 1D) and selected cDNAs shows that it possesses three alternative leader sequences; thus the gene appears to have three alternative promoters. These promoters should ultimately prove useful in the production of transgenic constructs for constitutive express

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00145.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractThe sarcomeric myosin heavy‐chain (sMHC) gene ofDrosophilais single‐copy and RNA transcription from this gene is developmentally regulated. Numerous sMHC mRNAs that differ in exon composition can be formed by alternate RNA processing. These transcriptional events result in the presence of multiple sMHC isoforms in the developing organism. We have developed and characterized two antibodies which are specific for different types of sarcomeric myosin heavy‐chain protein isoforms inDrosophilaand have begun to examine the tissue distribution and function of these various protein isoforms. One of the antibodies (anti‐A) is capable of distinguishing between two classes of sMHC protein isoforms which differ in their carboxy terminal amino acid sequences. The second antibody (anti‐MHC) recognizes a separate and distinct domain in sMHC protein isoforms. We demonstrate the specificity and the utility of these antibodies in examining the developmental and tissue‐specific expression of sMHC protein isoforms in the dev

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00146.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractCat fleas(Ctenocephalides felis)from eight commercial flea colonies from various regions of the USA were examined by selective PCR amplification, and subsequent restriction digest analysis and Southern hybridization of PCR products, for the presence of a rickettsia‐like organism (ELB agent). These flea colonies were either started with fleas from one supplier (EL Labs), in which ELB agent was first identified, or were started with fleas from stray cats and dogs and later came into contact with ELB‐infected fleas. Infection rates in the colonies ranged from 43% to 93%. The successful propagation of ELB agent in these colonies may be due to efficient trans‐stadial and transovaria) transmission. While ELB agent has recently been identified in blood from human murine typhus cases, attempts to infect mammalian cells and SCID mice with flea isolates were unsucce

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00147.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractTwo cDNAs are described fromAnopheles gamblaethat correspond to the ADP/ATP carrier or translocase. The clones are identical exceptfor minor differences in the 5 non‐coding region and in the lengths of the poly‐A tails. They code for mRNAs of 1261 and 1263 bp and contain one open reading frame of 906 bp. A probe made from the 1263 bp cDNA hybridized to bands of approximately 1260 and 1700 bp on developmental Northern blots. The putative 300 amino acid peptide sequence shows from 53.4–78.5% identity to AAC peptide sequences from a range of organisms fromZea maystohuman. Both clones mapped to region 26a on the left arm of chromosome 2 inAn. ga

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00148.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractA sensitive, simple, and reproduciblein situhybridization technique for the detection and precise localization of specific nucleic acid sequences on chromosomes of members of theAnopheles gambiaecomplex is described. Modifications of thein situhybridization technique are described that allow simultaneous hybridization of several probes with the chromosomes on a single slide and the multiple use of a single chromosome preparation for several different probes hybridized successively on the same slide. Examples are shown that illustrate the utility of the technique for localization of both single copy and repeated sequences in both polytenized euchromatin and centromeric heterochromatin.

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00149.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractA new family of retrotransposons (RTPs) without long terminal repeats (LTRs), designatedQ, has been isolated from the malaria vectorAnopheles gambiae.The nucleotide sequence of a complete element G‐22, was determined and analysed. Approximately 4.5 kb long, 0–22 contains two long overlapping open reading frames (ORFs) that potentially encode proteins with nucleic acid binding and reverse transcriptase domains similar to those of non‐LTR RTPs previously described. The 3 end is characterized by variable numbers of the triplet repeat TAA, immediately following a polyadenylation signal.In situhybridization of nurse cell polytene chromosomes revealed about twenty labelled sites distributed over all arms and diffuse hybridization to the chromocentre. Cross‐hybridizing sequences with the same internal structure occur in all members of theA. gambiaecomplex. Genomic Southerns of wildA. gambiaespecimens probed withQsuggest thatQis or recently was capable of retrotransp

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00150.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractWe used DNA reassociation kinetics to determine genome size and organization in the ixodid tickAmblyomma americanum.We calculated the genome size ofA. americanumto be approximately 1.08 pg or 1.04 × 109base pairs and to consist of 35.8% unique DNA, 4.2% foldback sequences, 17.9% highly repetitive sequences, and 42.1% moderately repetitive sequences. Comparison of the reassociation kinetics of long and short fragments revealed repetitive sequences to be distributed in a pattern of long period interspersion, a feature that, to date, has been associated with arthropod genomes that lack a high percentage of repetitive DNA

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00151.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractRibosomal DNA (rDNA) restriction fragment length of spider mites was analysed in three sibling species of genusPanonychus(P.mod, P. citriandP. ulmi)andTetranychus urticae.Seventy‐one fragments were detected by enhanced chemiluminescence (ECL) after Southern hybridization with two genomic rDNA probes of mouse. Percentages of shared fragments within species were larger than those among species, indicating that the variation of rDNAs was useful in phylo‐genetic studies of spider mite species. In a phenogram based on differences of the rDNA fragment length, the relationship amongPanonychusspecies was compatible with the results of analysis based on the morphological charact

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1994.tb00152.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY