摘要:

AbstractA diapause associated protein was electrophoretically isolated from the hemolymph of diapausing last instar larvae of the pink bollwormPectinophora gossypiella. This protein (Mr∼490,000, glycolipoprotein) was given the name Pectinophora diapause protein (PDP). It is composed of one subunit (Mr103,000). The concentration of PDP increased dramatically in the hemolymph of diapausing larvae from 17.4% in prediapause (PD) phase to 29.2% in early diapause (ED) phase reaching a level of 38.6% in larval hemolymph of middiapause (MD) phase. The concentrations of total proteins in the hemolymph of active feeding (A), PD, ED, and MD larvae were 69.8, 106.6, 113.3, and 118 mg/ml, respectively, while those in the fat body of the same larvae were 7.1, 7.4, 8.8, and 4.5 mg/g, respectively. InPectinophoraa drop in the concentration of fat body proteins coincided with a corresponding increase in hemolymph proteins, which suggests an active release of protein from the fat body into the hemolymph during the development of diapause. A partial amino acid sequence of pectinophorin showed the first 15 amino acids starting from the amino terminus of the peptide chain: N‐ALA‐LYS‐THR‐ILEU‐VAL‐GLU‐ASN‐MET‐PRO‐PRO‐THR‐PRO‐LEU‐ASN

ISSN:0739-4462    

DOI:10.1002/arch.940210102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractTrypsin modulating oostatic factor (TMOF), a decapeptide (H‐YDPAPPPPPP‐OH) that signals the termination of trypsinlike enzyme biosynthesis in the mosquito midgut, was covalently bound to Keyhole Limpet Hemocyanin using N‐hydroxysuccinimide and dicyclohexylcarbodiimide. Polyclonal antibodies raised in rabbits against this conjugate were used to develop specific RIA and enzyme linked immunosorbent assay (ELISA) to detect the peptide hormone in femaleAedes aegypti. TMOF and its analogs TMOF(B) (H‐DYPAPPPPPP‐OH), P4 (H‐YDPAPPPP‐OH), P1 (H‐YDPAP‐OH), and poly‐L‐proline were tested with the antiserum. The antiserum fully recognized TMOF and partially recognized P4. Using both RIA and ELISA, we report that the amount of TMOF in the mosquito ovary is 100 ± 20 ng (S.E.) and 96 ± 1.4 ng (S.E.), respectively, for each assay. Minute quantities of TMOF a thousandfold lower than in the ovary were found in the mosquito brain, indicating that the hormone is probably not neural but ovarian in origin.

ISSN:0739-4462    

DOI:10.1002/arch.940210103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractWe have studied two different established insect cell lines capable of synthesizing chitin, UM‐BGE‐4 from the cockroach and BRL‐AG‐2 from the boll weevil, for their ability to produce putative cuticle proteins. Mouse polyclonal antibodies were raised against the abdominal cuticle proteins extracted from the cuticle of nymphal and adult stage cockroaches, and larval, pupal, and adult stage cotton boll weevils. On Western blots, these antibodies detected several cross‐reacting proteins in untreated control cells and a variety of induced proteins in cells first exposed to a double pulse of the molting hormone, 20‐hydroxyecdysone (a low concentration followed by a higher concentration of hormone). The majority of these hormone induced proteins recognized by the anti‐cuticle protein antibodies were found to correspond in size to specific proteins observed in abdominal cuticle extracts. Electron microscopy utilizing colloidal gold to identify antibody binding sites indicated the antigenic proteins were present in the extracellular material found on the surface of the cultured cockroach and boll weevil cells. The evidence presented suggests that these cell lines can produce cuticle proteins. © 1992 W

ISSN:0739-4462    

DOI:10.1002/arch.940210104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractAn insect selective neurotoxic polypeptide from venom of the scorpionAndroctonus australis(AalT, Mr8,000) was shown to cross the midgut of the flesh flySarcophaga falculata, using assays of oral toxicity, column chromatography, and microscopic autoradiography of the native and radioiodinated toxin. AalT induced paralysis of flies within 1–2 h after oral administration, with a lethal dose (LD50) of 10 μg/100 mg of body weight. Oral toxicity was about 0.14% of toxicity by injection. Hemolymph collection 70–85 min after feeding flies with [125l]AalT showed that 5% of ingested radioactivity appeared in hemolymph. Most of this represented degradation products, but included about 0.3% of the chromatographically intact toxin. In contrast, hemolymph of identically treated lepidopterous larvae (Manduca, Helioverpa[=Heliothis]) contained degradation products but no intact toxin. [125l]AalT was shown to cross the midgut ofSarcophagathrough a morphologically distinct segment of the midgut previously shown to be permeable to a cytotoxic, positively charged polypeptide of similar molecular weight. These results suggest thatSarcophagamidgut contains a morphologically and functionally distinct segment that transports small peptides, and that employment of neurotoxic polypeptides for insect control may be feasible. Activity might be greatly improved through modification and metabolic stabilization of active peptides. © 1992 Wiley‐Li

ISSN:0739-4462    

DOI:10.1002/arch.940210105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractEgg maturation in the Indianmeal moth,Plodia interpunctella(Hübner), is initiated during adult metamorphosis. The temporal sequence for the initiation of vitellogenin (Vg) synthesis in fat body, the accumulation of Vg in hemolymph, and the accumulation of yolk proteins in terminal follicles during adult development are described. On the basis of quantitation by rocket immunoelectrophoresis, the amount of Vg in hemolymph prior to 80 h after pupation was below 50 ng/μl hemolymph. At 83 h after pupation, Vg was detectable at 0.4 μg/μl hemolymph and increased to over 20 μg/μl within 24 h after adult eclosion. Using immunofluorescent histochemical staining for Vg subunit yolk polypeptide 1 (YP1), the production of Vg was observed to increase rapidly in fat body between 96 and 100 h after pupation. Western blot analysis showed that YP2 was the first YP to accumulate in terminal follicles appearing at 96 h after pupation while the other three major YPs were first observed at 116 h. These findings demonstrate that initiation of vitellogenesis in terminal follicles begins around 96 h after pupation and involves the temporal coordination of fat body and follicle activities that provide Vgs before terminal follicles achieve competency to accumulate yolk proteins. © 1992 Wiley‐L

ISSN:0739-4462    

DOI:10.1002/arch.940210106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractThe soluble enzyme phenoloxidase (tyrosinase) from the larval cuticle ofLymantria disparhas been partially purified using Ultrogel ACA 34, and the activity has been determined using phenolic substrates. The enzyme exhibited more activity toward O‐diphenolic substrates and monophenolic substrates. The enzyme is inhibited by diethyl dithiocarbamate, phenylthiourea, and thiourea. The enzyme has been localized in the 7% slab and disc PAGE as an intense band. The enzyme is suggested to be involved in wound healing. © 1992 Wiley‐Liss,

ISSN:0739-4462    

DOI:10.1002/arch.940210107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

ISSN:0739-4462    

DOI:10.1002/arch.940210108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

ISSN:0739-4462    

DOI:10.1002/arch.940210101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY