摘要:

AbstractHeliothis virescensmaintained on a diet containing 50 ppm of thiazolylurea (5‐[[[2‐thiazolylamino]amino]carbonyl]‐1,3‐benzenedicarboxylic acid dimethyl ester) showed symptoms of juvenile hormone overdose in the last larval instar. Increases in development time and weight, as well as larval color changes, were similar in animals fed thiazolylurea or topically treated with the juvenile hormone mimic methoprene. The juvenile hormone esterase titer profile in hemolymph of animals fed thiazolylurea was much broader than in controls, and peaked 2 days later than in controls; the premolt ecdysteroid peak in hemolymph of animals fed thiazolylurea appeared 2 days earlier than in controls. These events are characteristic of high hemolymph JH titers throughout the last larval

ISSN:0739-4462    

DOI:10.1002/arch.940120202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractSex pheromone components of two species of arctiid moths,Estigmene acreaandPhragmatobia fuliginosa, were shown to be derived from linolenic acid. Female pupae were injected with radiolabeled malonic acid or an 18‐, 20‐, 21‐, or 22‐carbon triunsaturated fatty acid, and the pheromone components from emerged adults analyzed for radioactivity. The data support a biosynthetic pathway in which the 21‐carbon pheromone component,(Z, Z)‐3,6‐cis‐9,10‐epoxyheneicosadiene, of these moths is produced by chain elongation of linolenic acid to docosatrienoic acid with subsequent reductive decarboxylation. The 18‐carbon aldehyde components,(Z, Z)‐9,12‐octadecadienal and (Z, Z, Z)‐9,12,15‐octadecatrienal, ofE. acreaare produced from linoeic and linolenic acids directly. No detectable amounts of intermediate 20‐, 21‐, or 22‐carbon fatty acid precursors w

ISSN:0739-4462    

DOI:10.1002/arch.940120203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractApplication of methoprene to fourth (penultimate) instar larvae of the silkwormBombyx moriinduced the appearance of the feeding dauer larvae at the fifth (last) instar and prevented pupal metamorphosis. Methoprene also increased the protein concentrations of hemolymph last instar larvae by preventing sequestration of storage proteins by the fat body. Usually, the female‐specific storage protein 1 (SP1)* disappears from the male hemolymph at the time of the last larval instar. However, exposure of male larvae to methoprene at the penultimate instar enhanced the accumulation of SP1 in the hemolymph. The SP1 accumulated in males did not differ in molecular weight and immunoreactivity from the SP1 produced in female larvae. Both sexes of fourth instar larvae allatectomized on day 1 instantly accumulated SP1 in the hemolymph, and methoprene application after allatectomy suppressed the hemolymph accumulation of the SP1. In contrast, if allatectomy was carried out at a later stage of the fourth larval instar, SP1 concentration in hemolymph of fifth instar larvae did not increase, suggesting the different juvenile hormone action for regulation of SP1 synthesis in the penultimate instar larvae of silkworm

ISSN:0739-4462    

DOI:10.1002/arch.940120204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractProtein synthesis is cyclic during pupal diapause inSarcophaga crassipalpis. These cycles are in phase with infradian MO2cycles, which have a periodicity of about 4 days at 25°C. Mean incorporation of [35S]methionine by diapausing pupae was 5.4% during the 2 days of highest MO2but dropped to 1.7% during the 2 days of low MO2. Diapausing pupae treated with a juvenile hormone analog prior to pupariation had a constant high MO2similar to peak values observed in untreated pupae, and such pupae consistently incorporated [35S]methionine at a high rate (7.7%). [35S]Methionine incorporation by nondiapausing pupae and pharate adults was eightfold higher than the peak rates observed during diapause. Autoradiography of in vivo labeled proteins indicated quantitative and qualitative changes in the synthesis of proteins by diapausing pupae during different phases of the MO2cycle. Brains from diapausing pupae labeled in vitro showed higher incorporation at the peak of the MO2cycle than at the nadir of the cycle, but no such differences were detected for integument, fat body, or fat body supernatant. Theses differences in tissue response indicate that control of protein synthesis during diapause is not cell autonomous, but is a function of the metabolism of the intact organism

ISSN:0739-4462    

DOI:10.1002/arch.940120205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe fatty acids of the triacylglycerol reserves in the fat body and of the diacylglycerol of lipophorin in the hemolymph of non‐diapause and diapause larvae ofD. grandiosellawere compared. For both non‐diapause and diapause larvae palmitate, palmitoleate, oleate, and linoleate were the predominant fatty acids present in fatty body triacylglycerol, and palmitate, oleate, and linoleate were the predominant fatty acids present in lipophorin diacylglycerol. However, differences were detected in the relative amounts of oleate and linoleate present in lipophorin diacylglycerol of non‐diapause and diapause larvae. The relative amount of linoleate in lipophorin diacylglycerol declined during diapause, whereas that of oleate remained relatively high during diapause. The fatty acid profile of lipophorin diacylglycerol from non‐diapause larvae treated with a juvenile hormone analog to induce a diapause‐like state more closely matched that of diapause larvae than that of non‐diapause larvae. The differences detected in the fatty acid composition of lipophorin diacylglycerol in non‐diapause and diapause larvae appear to be due mainly to the different physiological states rather than to the different rearing temperatures employed. The results are discussed in relation to the essential role fatty acids, especially oleate, play in the survival of di

ISSN:0739-4462    

DOI:10.1002/arch.940120206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

ISSN:0739-4462    

DOI:10.1002/arch.940120201

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY