摘要:

AbstractThe major ecdysteroids in large worker pupae of the leaf‐cutting antAcromyrmex octospinosuswere characterized at the peak ecdysteroid concentration by using high‐performance liquid chromatography, enzyme immunoassay, and mass spectrometry. In decreasing amounts, they were determined to be makisterone A, an unidentified C28ecdysteroid bearing a molecular weight of 494, 20‐hydroxyecdysone (ratio of 1 to 6 as compared to makisterone A), and putative but negligible ecdysone. The presence of both C28and C27ecdysteroids is discussed in relation to the content of 4‐desmethylsterols determined by gas chromatography and mass spectrometry to be ergosta‐5,7,24 (28)‐trien‐3β‐ol, ergosterol, ergosta‐5,7‐dien‐3β‐ol and ergosta‐7,24(28)‐dien‐3β‐ol for the main sterols, and

ISSN:0739-4462    

DOI:10.1002/arch.940160102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

摘要:

AbstractRH 5849 competes with3H‐ponasterone A for ecdysteroid binding sites inChironomus tentanscells with an about fourfold lower relative affinity as compared to 20‐OH‐ecydsone. It does not interfere with glucocorticoid and estrogen binding sites in vertebrates. It is also not recognized by an ecdysteroid antibody. RH 5849 exerts typical morphological and physiological effects ascribed to the action of 20‐OH‐ecdysone onC. tentanscells, namely an increase in acetylcholinesterase activity and an inhibition of chitin

ISSN:0739-4462    

DOI:10.1002/arch.940160103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

摘要:

AbstractThe accumulation and biosynthesis of cuticular and internal hydrocarbons in the Southern armyworm,Spodoptera eridania, were examined at closely timed intervals during larval and pupal development. Gas chromatography‐mass spectrometry (GC‐MS) was used to identifyn‐alkanes, monomethylalkanes, and dimethylalkanes ranging in chain length from 23 to 35 carbons. The amount of cuticular hydrocarbon stayed relatively constant during each stadium, while the amount of internal hydrocarbon increased dramatically during the first half of each larval stadium, presumably to replace the cuticular hydrocarbon lost on the shed cast skin with each molt. The accumulation of internal hydrocarbon was mirrored by large increases in the rate of incorporation of labeled acetate into the hydrocarbon fraction. Hydrocarbon production fell to very low rates during the latter part of the fourth and fifth larval stadia. Relatively high rates of hydrocarbon production were observed during the first and last one‐third of the pupal stage and essentially all of the hydrocarbons produced during this stage remained internal. These data document large changes in the rates of hydrocarbon production during development inS. eridaniaand suggest that most of the hydrocarbon produced during each stage was stored internally and then transported to the cuticle of the nex

ISSN:0739-4462    

DOI:10.1002/arch.940160104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

摘要:

AbstractThe oxidation of 3,4‐dihydroxybenzyl alcohol, one of the sclerotizing precursors for the tanning of the ootheca of cockroachPeriplaneta americana, is reported for the first time. Mushroom tyrosinase catalyzed oxidation of 3,4‐dihydroxybenzyl alcohol generated the corresponding quinone which was found to be unstable and readily transformed to produce 3,4‐dihydroxybenzaldehyde as the stable product probably through the intermediary formation of a quinone methide. Phenoloxidase isolated from the left colleterial gland ofP. americanaalso catalyzed this new reaction. When the enzymatic oxidation of 3,4‐dihydroxybenzyl alcohol was performed in the presence of a test protein such as lysozyme, the reactive species formed, caused the oligomerization of test protein. Similar studies with colleterial gland proteins, failed to generate oligomers, but produced insoluble polymeric proteins. The probable fate of 3,4‐dihydroxybenzyl alcohol for the tanning of cockroach ootheca is

ISSN:0739-4462    

DOI:10.1002/arch.940160105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

摘要:

AbstractA segment of the house fly(Musca domestica)homologue of thepara (paralytic)sodium channel gene ofDrosophila melanogasterwas isolated by using mixed sequence oligonucleotide primers in the polymerase chain reaction (PCR). The specificity of the procedure was demonstrated by genomic Southern analysis using the housefly PCR amplification product as a probe and by DNA sequence analysis. The latter showed structural homology to theparagene, but not to the corresponding region ofDSC1, anotherD. melanogastergene with structural similarity to vertebrate sodium channel genes.

ISSN:0739-4462    

DOI:10.1002/arch.940160106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

摘要:

AbstractRibosomal subunits have been prepared from larvae ofCeratitis capitataat two different developmental stages. The subunits are stable and pure on the basis of recentrifugation experiments and characterization of the RNA contained in them. When ribosomal subunits were allowed to combine, they reassociated to form an 80S ribosome, which is active in poly(U)‐directed polyphenylalanine synthesis. The proteins derived from these subunits were characterized by one and two dimensional electrophoresis and the patterns obtained from 6‐day old larvae and white prepupae were compa

ISSN:0739-4462    

DOI:10.1002/arch.940160107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

ISSN:0739-4462    

DOI:10.1002/arch.940160108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY

ISSN:0739-4462    

DOI:10.1002/arch.940160101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1991

数据来源: WILEY