ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01158.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTInvolved and noninvolved epidermis from 15 psoriatic patients, and epidermis from 12 normal individuals were grown under various conditionsin vitroas cell and organ cultures. In the organ cultures of normal or noninvolved psoriatic skin, epidermal cells migrated around the dermis and differentiated. In psoriatic organ cultures, progressive necrotic changes were observed in the spinous cells including gradual detachment of the spinous layer from the dermis. In spite of these necrotic changes, newly‐grown basal cells which appeared on the dermal tissue in the 9 to 12‐day psoriatic explants clearly incorporated3H‐thymidine into nuclear DNA. By electron microscopy these cells were observed to have numerous ribosomes, a few mitochondria, tonofilament‐like structures in the cytoplasm, and no hemi‐desmosomes. Dissociated epidermal cells from the involved psoriatic skin failed to grow on plastic dishes. When these cells were plated on dishes coated with increasing amounts of collagen, the rate of cellular attachment was proportional to the thickness of the collagen coating, but they were unable to grow and form epidermal monolayers. In contrast, normal and noninvolved psoriatic epidermal cells attached to the dishes regardless of the presence of collagen and formed differentiated epidermal m

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01159.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTSerum levels of Etretinate (ET, Ro 10‐9359) and its metabolite (AM, Ro 10‐1670) were monitored during and after cessation of ET treatment in 12 patients with acquired disorders of keratinization who underwent the ET therapy at initial doses of 30 to 60 mg/day. Studies were made of the possible correlations between ET and AM serum levels, the appearance and disappearance of side effects, and elimination time profiles. Side effects appeared 1 to 2 weeks after the initiation of ET administration in all cases, although serum ET and AM levels at this time varied considerably, indicating individual differences in ET and AM susceptibility in the appearance of side effects. Serum ET and AM levels at the time of disappearance of side effects were lower than those at the time of appearance, suggesting some correlation of side effects with serum levels of ET, AM, or both. Serum level curves for ET and AM after cessation of ET administration were diverse. The maximum ET and AM elimination times from serum were 3 and 6 months, respectiv

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01160.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe stratum corneum obtained from seven cases of bullous congenital ichthyosiform erythroderma (BCIE) and one case of Vörner type keratosis palmaris et plantaris (KPP) was fractionated into keratin fibers, soluble proteins, and cell membranes. To study the fractions, SDS polyacrylamide gel electrophoresis was used for the keratin fibrous and soluble fractions and amino acid analysis was employed for the membraneous fraction. In the electrophoresis of the keratin fibrous fractions, the 55,000 dalton band was either absent or greatly reduced in BCIE, whereas almost normal patterns were obtained in Vörner type KPP. The soluble fractions from BCIE demonstrated abnormal patterns in different cases on electrophoresis. Amino acid compositions of the keratin fibrous and membraneous fractions in BCIE were identical to those of the normal control. It is suggested that, as the 55,000 dalton constituent of keratin fibrous protein is absent or greatly reduced, this is a specific characteristic of BCIE, and that biochemical changes differ for BCIE and Vörner type KPP, which have been thought to be essentially identical due to their histological and electron‐microscopic simila

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01161.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTSilver stains are well known as reliable for demonstrating Treponema pallida (T.P.) in the tissues, but sometimes it is difficult to distinguish T.P. from the tissue elements in tissue sections. We now present a new method for demonstrating T.P. in the tissues using FTA‐ABS complement staining and scanning electron microscopy in the same materials embedded in paraffi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01162.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA comprehensive survey of onchocerciasis was made at San Vicente Pacaya, Guatemala, in 1978 to determine whether a relationship exists between general cutaneous changes and specific ones in Guatemalan onchocerciasis, and whether the depigmentation on the lower extremities is an onchocercal specific change or not. A total of 1185 people, 623 males and 562 females, were examined. The prevalence rate of onchocerciasis in the area surveyed was 41.6% in males and 17.3% in females. The prevalence rate of nodules was 20.1% in males and 6.8% in females. There was a similarity in the age distribution curves between the prevalence rate of nodules and that of onchocerciasis. The prevalence rate of the depigmentation on the lower extremities was 4.8% in males and 6.4% in females. There was no correlation between the age distribution curve and the prevalence rate of onchocerciasis. Symptoms coincident to the so called “pretibial depigmentation” were seen in 5 of 66 depigmented cases. The prevalence rate of the cicatricial depigmentation on the lower extremities was 31.3% in males and 28.3% in females. The rate of this depigmentation was considerably higher than the non‐cicatricial depigmentation of the lower extremities.The prevalence rate of lymphadenopathy was 59.8% in males and 9.8% in females. “Hanging groin” and elephantiasis were not seen in this region of Guatemala. The prevalence rate of all eczematous dermatitis was 11.3% in males and 9.8% in females. There were six cases with generalized eczematous dermatitis among our series of 128 cases. Five cases were seen in patients with onchocerciasis. Except for lymphadenopathy, our data shows the prevalence rate of cutaneous changes to be relatively low when compared to the prevalence rate of Guatemalan onchocerciasis. The prevalence rate of the generalized eczematous dermatitis, which may be similar to onchocercal dermatitis, is extremely low, and the depigmentation on the lower extremities, which may be similar to the pretibial depigmentation, is not commonly seen in this region of Guatemala. However, similar mild conditions not coinciding with the typical case showed a considerably higher prevalence rate in the same surveyed region. The correlation between the prevalence rate of the lymphadenopathy and the onchocerciasis infection rate appeared to be real. From these results, we feel that palpation of lymph nodes is useful for the determination of clinical information in onchocerciasis, without causing any significant discomfort to the patients being

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01163.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTFrom the present experiments in which sensitization was induced by painting contactants on the same skin of the same mouse over time, the following findings were observed.When mice were sensitized first with PCl, then with DNFB, and finally with OX, the animals showed a normal contact hypersensitivity response to PCl, but abnormal responses to both DNFB and OX, suggesting some suppressor phenomenon.This suppression was shown to be partly due to hapten (DNFB)‐specific suppressor cells, and it was suggested that other suppressor cells different from suppressor T cells in nature might be involved.It was considered that induction of suppressor cells might be due to depressed function of the epidermal Langerhans cell affected by potent sensitizers as well as by unknown mechanism

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01164.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTTwenty warts includingTinea versicolorlike lesions of epidermodysplasia verruciformis were examined for cell membrane associatedβ2microglobulin by the use of the direct immunofluorescence and indirect immunoperoxidase technique. The human papilloma virus infected keratinocytes in the granular layer and the uppermost stratum spinosum lack immunoreactiveβ2microglobulin on the cell surface. This finding indicates that the human papilloma virus does alter the cell membrane properties of the infected target keratinocyte, and the failure to detectβ2microglobulin on cell surface does not necessarily mean that these cells have undergone malignant transformati

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01165.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe interaction between chlorpromazine (CPZ) and riboflavin binding protein (RBP) was studied by the spectrophotometric and spectrofluorometric methods.CPZ combined with RBP in a 1:1 molar ratio and the dissociation constant was determined to be 3.8 × 10–5M at pH 7, 25°C. Flavins such as riboflavin (Rf) and lumiflavin were released from the complexes with RBP by the addition of CPZ. The mechanism of flavin‐release was mainly competitive binding between CPZ and a isoalloxazine ring of flavins to RBP.The mechanism of some biological phenomena induced by CPZ was discussed based on the flavin‐CPZ inte

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01166.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTBCG, PHA, PWM, PPD and LPS‐Su all enhanced the natural killer‐like cytotoxicity of the nylon wool‐treated human lymphocyte fraction against a human skin squamous cell line (HSC‐1)in vitro. The cytotoxicity was measured by51Cr release from the target cells (HSC‐1) when they were mixed with lymphocytes preincubated with or without the stimulators for three days. The activity of BCG and PPD in enhancing the lymphocyte cytotoxicity was stable after sonication and heating, although it was slightly lowered after treatment of BCG with streptomycin.The K‐562 cell line, which has been supposed to be specifically sensitive to the natural killer activity, proved to be also sensitive to the cytotoxic effect of the nylon wool‐treated lymphocyte fraction enhanced by BCG and PHA as w

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01167.x

年代:1983

数据来源: WILEY