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1. |
HETEROGENEITY OF EPIDERMAL PREKERATIN AND KERATIN BETWEEN SOLE AND BODY SKIN IN HUMANS |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 211-218
Yasuo Kitajima,
Hiroko Furuta,
Yoriko Tsuneda,
Kazufumi Yoneda,
Shunji Mori,
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摘要:
ABSTRACTRecently, heterogeneity of stratum corneum keratin (α‐fibrous protein) in various anatomic sites has been described in humans as well as cows and rats. In order to study this heterogeneity more clearly in humans, we compared acid soluble (citrate buffer at pH 2.65) prekeratin, acid insoluble (8 M urea‐2‐mercaptoethanol soluble) living cell keratin, and stratum corneum keratin from sole and body (nonsole‐nonpalm) skin using SDS‐polyacrylamide gel electrophoresis (PAGE), amino acid analysis, and immunological analyses.In prekeratin, no differences were seen between sole and body by SDS‐PAGE. Prekeratins from the two sources showed a 4‐band pattern with molecular weights of 55,000, 59,000, 62,000 and 69,000. In living cell keratin, the 69,000 band disappeared and a 49,000 band appeared in sole, but not in body epidermis. In stratum corneum keratin, a distinct difference was seen between sole and body skin. The sole stratum corneum keratin showed a 3‐band pattern (55,000, 59,000 and 62,000 mw) while that of body showed a two‐band pattern (59,000 and 66,000 mw).Despite the heterogeneity of the SDS‐PAGE profiles, no significant differences were seen in amino acid composition amongst these specimens.Immunologically, using double diffusion, antiserum against stratum corneum keratin from the sole principally produced two precipitin lines against the same keratin of sole origin and one precipitin line against prekeratin, living cell keratin, and stratum corneum keratin of body origin. This precipitin line fused with one of the two precipitin lines produced between antiserum and the sole stratum corneum keratin.These results indicate that anatomical‐site heterogeneity in α‐fibrous protein of human epidermis does not exist at the acid‐soluble prekeratin level, but does at the acid‐insoluble keratin level of living cell layer and stratum corneum when examined by SDS‐PAGE, and that some, but not all, keratin antigens are immunological
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01468.x
年代:1984
数据来源: WILEY
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2. |
STATISTICS OF NEVI IN 3‐YEAR‐OLD JAPANESE CHILDREN |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 219-224
Ichiro Kikuchi,
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摘要:
ABSTRACTA total of 1319 3‐year‐old Japanese children were examined for nevi. Moles (small nevocellular nevi) were found in 40% of those examined; blue nevi could not be found at this age. Common Mongolian spots (Mongolian spots over the back and buttocks) were found in 94.8% and aberrant Mongolian spots (Mongolian spots appearing in other areas) in 4.4%. Brown Mongolian spots, a phenomenon appearing in some of those in the disappearing phase of Mongolian spots, appeared in 15.8%. Salmon patches on the eyelids and forehead had disappeared by age 3, but some regressing strawberry marks could be observed at this
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01469.x
年代:1984
数据来源: WILEY
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3. |
THE USE OF A METAL HALIDE LAMP FOR THE INDUCTION OF PHOTOHEMOLYSIS IN PORPHYRIA |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 225-232
Tetsuzo Honda,
Shigeo Nonaka,
Taro Ohgami,
Fumio Murayama,
Hikotaro Yoshida,
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摘要:
ABSTRACTSeveral light sources, including sunlight, xenon lamps, fluorescent lamps, mercury lamps and carbon arc lamps, have been used to provoke skin changes for phototests in patients with porphyrias, or to induce experimental hemolysis of porphyrins for research. The metal halide lamp has a strong emission range between 400 nm and 450 nm, and a relatively low emission range in the ultraviolet region. For this reason, we explored the possibility that this lamp could be used to induce photohemolysis caused by hematoporphyrin (HP) and the radiation of light. Twenty to forty μg of HP‐HCl was added to 25 ml of a normal red blood cell suspension. The flask containing this red blood cell suspension was then irradiated using a metal halide lamp with 3.6 to 10.8 J/cm2of light. All of the irradiated red blood cell suspension was hemolyzed, but the non‐irradiated control showed very little hemolysis.Compared to natural sunlight, the energy emitted by the metal halide lamp is small. Therefore, a longer exposure from the metal halide lamp is required to obtain an amount of energy equivalent to sunlight. This type of light source covers a wide area for irradiation. From these results, we can speculate that a metal halide lamp source is of value when used experimentally to produce the chronic skin changes of porph
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01470.x
年代:1984
数据来源: WILEY
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4. |
EFFECTS OF SERUM ALBUMIN ON COAGULASE RELEASE FROMSTAPHYLOCOCCUS AUREUSDERIVED FROM BULLOUS IMPETIGO |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 233-235
Satoshi Dekio,
Joji Jidoi,
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摘要:
ABSTRACTThree strains ofStaphylococcus aureusderived from bullous impetigo were cultured in media either not containing or containing serum albumin. The coagulase titers in each medium were measured as a function of the cultivation time. In the early‐mid phase of growth, the coagulase titers were increased to the same degree in both media. After that, the coagulase titers were maintained at the high level in the medium containing serum albumin but decreased in the medium not containing serum albumin. It is proposed thatStaphylococcus aureusstrains capable of causing bullous impetigo infect the injured skin surface with accumulation of plasma‐like material, and then grow with continuous release of coagulase. Under the protection of the fibrin membranes formed by the coagulase, the bacteria are established, and then the bullous lesions are for
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01471.x
年代:1984
数据来源: WILEY
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5. |
DISTURBED MITOTIC PROCESSES OF STROMA CELLS IN A PATIENT WITH TUBEROUS SCLEROSIS |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 236-252
Yasumasa Ishibashi,
Yukiko Inoue,
Kazuhiko Takehara,
Masutaka Furue,
Atsushi Kukita,
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摘要:
ABSTRACTSkin fragments of a giant molluscum pendulum and so‐called adenomata sebacea (AS) in the face of a 21‐year‐old female with Pringle's disease were explanted and the cell divisions of outgrown and subcultured non‐epithelial cells (NECs) were observed at suitable intervals with a phase contrast microscope. In both tissues, a number of NECs, especially large and medium sized ones, showed distinct chromosome dysarrangements, including segmental arrangement, eccentric arrangement, and arrangement failure, when chromosomes were arranged in the central area of individual cells in metaphase, causing extreme lagging in the subsequent cell division.These cells showed varying disturbances such as 1) the formation of elevated humps of varying sizes, followed by division after some of them were eliminated, 2) division into two cells of different sizes, 3) division into three after microcells had formed, 4) division into several, which was followed by restitution into two by fusion, 5) division failure, and 6) multinucleation after division, all of which suggested C‐mitosis.These findings indicate that the distributing mechanism of chromosomes,i.e.the centromere‐microtubules‐centriole system, particularly the function of microtubules, might be disturbe
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01472.x
年代:1984
数据来源: WILEY
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6. |
LABORATORY STUDIES OF COLLAGEN WOUND DRESSING (CAS) |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 253-258
Shinjiro Murata,
Masao Fujigaki,
Koshiro Umemura,
Masanori Izawa,
Uichi Shibata,
Tadahiko Fujii,
Masaru Nishizawa,
Kuniharu Iwamoto,
Katsumi Matsumoto,
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摘要:
ABSTRACTIn order to elucidate the behavior of collagen wound dressing (CAS) after application, the transport of radioactivity from3H‐CAS applied to rabbit skin excision was determined.Neutral soluble collagen fraction was extracted from the skin of 30 rats given 30 mCi of3H‐glycine, followed by crosslinking with glutaraldehyde. The resulting mixture was then used as a binder of unlabelled collagen web to form3H‐CAS.Skin, 2.5 cm square and 0.02 inch deep, was excised with a dermatome on bilateral sites of the back of 6 rabbits.Then,3H‐CAS sheet, 3.5 cm square, was applied to each excision with 20 pieces of gauze and an elastic bandage. Each animal was kept separately in a metabolic cage to collect urine and feces every 24 hours. After the animals were sacrificed on the 8th day,3H‐CAS, gauze, elastic bandage, and the skin near the treatment area were collected, and the radioactivity was determined by a liquid scintillation counter after combustion in a sample oxidizer.More than 98.2% of radioactivity was recovered from3H‐CAS after 8 days.Release of radioactivity from3H‐CAS by exudate was negligible and percutaneous transport of collagen was not observed.No radioactivity was detected in rabbit serum, ur
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01473.x
年代:1984
数据来源: WILEY
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7. |
POSSIBILITY OF PASSIVE TRANSFER OF COLD URTICARIA TO GUINEA PIGS |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 259-262
Ichiro Katayama,
Toshiaki Doi,
Kiyoshi Nishioka,
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摘要:
ABSTRACTSerum from one out of six patients with idiopathic acquired cold urticaria produced a short lived edematous reaction in the pinna of guinea pigs 24 hours after intradermal injection of the patient's serum and subsequent cold challenge. No relationship was observed between the transfer activity and IgE titer or atopic status.
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01474.x
年代:1984
数据来源: WILEY
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8. |
INITIAL STAGE OF PROCESSING FROM PREKERATIN INTO KERATIN MOLECULES IN HUMAN EPIDERMISIN VITRO |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 263-268
Yohtaro Katagata,
Kazuo Aso,
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摘要:
ABSTRACTIn order to clarify the initial formation of keratin from prekeratin molecules, we undertook a pulse‐chase experiment using [U‐14C]‐glycine labelled prekeratin subunit mixturein vitro.The experiment was carried out at 37°C for 3 hours using a specimen (3 mm2×0.1 mm) in Dulbecco minimum essential medium (MEM) containing14C‐glycine. Incorporation of14C‐glycine into the prekeratin molecules was exhibited in six prekeratins (M.W. about 71, 69, 62, 55, 52 and 49 K, respectively), and the highest incorporated molecule among them was the 55K‐prekeratin molecule. After 30 minutes at 37°C in the above medium, the intact specimens were transferred into a comparable medium free of14C‐glycine, and further incubated for 4 hours. The 52K‐ and 62K‐prekeratin molecules had increased at 60 minutes after being chased, as compared with the starting radioactive value. From this result, it was suggested that the two prekeratin molecules might be intermediates in the initial stage of keratinization. Moreover, the human epidermal homogenate was more effective in converting the prekeratin into keratin molecules. Some factor(s) may be present in the fraction which convert the prekeratin in
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01475.x
年代:1984
数据来源: WILEY
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9. |
LOCALIZED SCLERODERMA (MORPHOEA) |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 269-276
Jørgen Serup,
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摘要:
ABSTRACTThe function of eccrine sweat glands was studied in 12 patients with morphoea by two methods: paper‐disc collection of sweat following pilocarpine iontophoresis, and microscopical counting of sweat pores in a rubber base imprint taken with a material with a comparatively long cure time (Coe‐flexRregular). Control samples were obtained from normal appearing skin of the same individuals. In morphoea plaques, the secretion of sweat per 30 minutes as well as the number of sweat pores per cm2were reduced (p<0.01, p<0.01). The correlation between the two methods was poor. After pilocarpine iontophoresis, the sweat pore count decreased in scleroderma skin (p<0.01) indicating gland fatigue. Concentrations of sodium and potassium in sweat appeared normal in the plaques as well as in the control sites. Results of the experiments were, altogether, in agreement with the histology showing sweat gland atrophy.Skin microtopography microscopy showed absence of the normal crease pattern to be an obligatory finding in morphoea plaques, sometimes with longitudinal orientation of creases in surrounding skin.Microscopy of skin surface imprints may be valuable as a supplementary method for assessment of skin and sweat gland affliction in sclerode
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01476.x
年代:1984
数据来源: WILEY
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10. |
COMPARISON OF THEIN VITROANTIFUNGAL ACTIVITIES OF CLOTRIMAZOLE, MICONAZOLE, ECONAZOLE AND EXALAMIDE AGAINST CLINICAL ISOLATES OF DERMATOPHYTES |
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The Journal of Dermatology,
Volume 11,
Issue 3,
1984,
Page 277-281
Toshio Kusunoki,
Seiichi Harada,
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摘要:
ABSTRACTThein vitroantifungal activities of clotrimazole, miconazole nitrate, econazole nitrate, and exalamide against 64 clinical isolates of various dermatophyte species obtained from out‐patients seen at the Nippon Medical School Hospital were simultaneously compared using a liquid microculture method. The rank order of activity against the 64 isolates of dermatophytes was clotrimazole>econazole nitrate>miconazole nitrate>exalamide. For a given antimycotic, the minimal inhibitory concentration was affected by the dermatophyte specie
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1984.tb01477.x
年代:1984
数据来源: WILEY
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