摘要:

ABSTRACTLysozyme was isolated from hairless mouse skin by extraction with 0.1 N acetic acid containing 0.9 percent NaCl. Crude enzyme was purified using successive chromatographic processes on carboxymethylcellulose and Sephadex G‐50. Purified hairless mouse skin lysozyme was found to have a homogeneous electrophoretic migration pattern on polyacrylamide gel.The amino acid composition and several physico‐chemical properties of hairless mouse skin lysozyme were similar to those of human skin lysozyme, but different from those of hen egg white lysozyme. The antigenic properties of hairless mouse skin lysozyme were also closely related to those of human skin lysozyme, but not to those of hen egg white lysoz

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1974.tb00921.x

年代:1974

数据来源: WILEY

摘要:

ABSTRACTCollagen‐like protein (CLP) was demonstrated in the serum of normal human individuals and of patients with connective tissue disorders. The average amount of CLP in normals was 9.1 ± 0.2 μg/ml, whereas in systemic scleroderma, circumscribed scleroderma, and systemic lupus erythematosus the amounts were significantly lower. Serum CLP levels were also found to be decreased in pseudoxanthoma elasticum, striae atrophicae, and impetigo herpetiformis, but variable in dermatomyositis, and within normal limits in pyoderma gangrenosum. There seemed to be a relationship between the behavior of collagen‐like protein and the severity of clinical manifestations in systemic scleroderma and systemic lupus erythema

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1974.tb00922.x

年代:1974

数据来源: WILEY

摘要:

ABSTRACTThe incidence of psoriasis was determined in 21,441 patients of dermatologic population, of which 142 were psoriasis, giving its incidence as 0.66 percent. The mean age in the males and females was computed as 33.1 and 33.7 years respectively, while the age at onset of the disease was almost similar in males and females. The emotional undertones were the major provocating factors, although trauma, parturition and infection were incriminated in a few cases. In 4.9 percent of psoriatics, the family history was suggestive. The duration of the disease was varying from a few days to a few years, more than 50 percent however reported before a years. Remissions and exacerbations were noticed in many patients with or without treatment, but seasonal variation had no significant influence on the course of the disease. Classical papulosquamous cutaneous rash and associated itching were the salient clinical features, although joint pains were featured in a few. Pitting, thickening and subungual hypertrophy were the main salient features in the nails. Very few patients had manifested clinical joint involvement, swelling of the distal interphalangeal joints being the commonest. Other associated diseases were rarely seen in these patients.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1974.tb00923.x

年代:1974

数据来源: WILEY

摘要:

ABSTRACTThe histochemical property of colloid was studied in two cases of colloid milium. Alcian blue, azure A, and periodic acid Schiff were used to stain mucopolysaccharides. Bromphenol blue was employed for detection of basic proteins. In a further attempt to identify various polyanions histochemically, staining was carried out with alcian blue containing various concentrations of electrolytes. Methylation, saponification, mild acid hydrolysis, and digestion with streptomyces or testicular hyaluronidase, or chondroitinase ABC were also employed. The results obtained indicate that the colloid in the present cases contains sulfated acid mucopolysaccharide, probably chondroitin sulfate C.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1974.tb00924.x

年代:1974

数据来源: WILEY