摘要:

AbstractCirculating anti‐basement membrane zone (BMZ) antibodies in a patient with cicatricial pemphigoid (CP) were examined using an indirect immunofluorescence test, indirect immunoperoxidase electron microscopy, and Western blot analysis. An indirect immunofluorescence test on salt‐split skin revealed that the anti‐BMZ antibodies reacted solely to the dermal side at the separating epidermal‐dermal interface, and indirect immunoelectron microscopy on intact skin indicated localization of the corresponding antigens (CP antigens) over the lamina densa and within the lower half of the lamina lucida; there were no CP antigens beneath a melanocyte. Indirect immunoelectron microscopy on salt‐split skin demonstrated that the CP antigens were partly dissociated from, but restricted to, the lamina densa. Western blot analysis showed no differences in molecular weight between the CP antigens and bullous pemphigoid (BP) antigens. CP antigens, as detected by this patient's serum, appear to be constituted of molecules quite similar to BP antigens, but with different epitopes. CP antigens may be shed from basal cells and locate in the area of anchoring filaments, where they play a role in connecting basal cells to the underlying lam

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03260.x

年代:1992

数据来源: WILEY

摘要:

AbstractIn vivoexperiments have shown that magnesium deficiency elicits characteristic skin lesions in the hairless rat. However, the mechanism of the involvement is not clarified. From the results of previous studies, a product derived from arachidonic acid, but notviathe cyclooxygenase pathway, has been considered as an etiological cause.In this study, the inhibitory effect of newly produced leukotriene B4(LTB4) receptor antagonist (LTBRA) against these skin manifestation was examined in hairless mutants given a hypomagnesic diet. Control animals with treatment of LTBRA developed eruptions on their bodies with increasing serum levels of LTB4, but LTBRA‐treated animals did not suffer from the cutaneous disorder. Increased serum levels of LTB4in control rats were significantly higher than those in the pre‐treated condition. The diminution of skin lesions by LTBRA strongly indicated that magnesium deficiency dermatitis may involve a lipoxygenase‐mediated metabolite of arachidonic acid, presumably LTB4, and that the cutaneous changes in hairless rats fed with hypomagnesic diet might provide an easily identifiable indicator of LTB4‐mediated der

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03261.x

年代:1992

数据来源: WILEY

摘要:

AbstractIn vivostudies in rodents suggest that prostaglandins and/or leukotrienes are involved in the epidermal induction of ornithine decarboxylase (ODC). Recently, we have shown that, in human epidermis, prostaglandins are not involved in this process. Here we report the role of leukotrienes in epidermal ODC induction in human skin. Topical flufenamic acid (DignodolinR), vehicle, or nothing was applied under plastic occlusion to three sites on the backs of healthy volunteers. This was followed 1 h later by SellotapeRstripping. After renewed application and occlusion for 8 h, biopsies were carried out for the estimation of ODC levels. There were no significant differences in the levels of ODC between the flufenamic acid treated and control sites. To confirm this finding, test sites were irradiated with 3 MED of UVB. This was immediately followed by the application of flufenamic acid, vehicle, or nothing to the three irradiated sites. After 8 h, biopsies were taken, and the levels of ODC were again similar in the flufenamic acid‐ and the vehicle‐treated sites. The data indicate that, following SellotapeRstripping or UVB irradiation, neither lipoxygenase nor cyclooxygenase products contribute to thein vivoinduction of ODC in human epider

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03262.x

年代:1992

数据来源: WILEY

摘要:

AbstractSun protection factors (SPFs) were evaluated with three light sources (sunlight, a xenon arc solar simulator, and fluorescent lamps) in indoor and outdoor studies. Two types of light, UV‐A+B and UV+Visible, were obtained from the solar simulator. The untanned backs of twenty‐four healthy male volunteers were used as test sites. A broad spectrum sunscreen containing SPF 6, according to the manufacturer, was used. The sunscreen tested was applied at 2 mg/cm2. The actual SPF values were 4.8 with sunlight, 6.0 with UV‐A+B, 4.9 with UV+Visible, and 11.8 with fluorescent lamps. There were no significant differences between the SPF values with sunlight and those with the solar simulator; the SPF value for fluorescent lamps was significantly higher. The SPF with UV‐A+B of the solar simulator was similar to that with sunlight; the use of this light served to reduce pain on tested subjects. Therefore, UV‐A+B from the solar simulator seems to be the most appropriate artificial light source for evaluating s

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03263.x

年代:1992

数据来源: WILEY

摘要:

AbstractWe conducted a statistical review of 114 cases of dermatomyositis (DMS) treated primarily at the Department of Dermatology at Nagoya University Hospital over 27 years from 1965 to 1991 in order to determine the primary characteristics of juvenile DMS with the following results.1)Juvenile DMS was found slightly more often in males than females; the male‐to‐female ratio was 1.4: 1. Therefore, unlike adult DMS with its preponderance of females, there was no clear gender predominance.2)Muscular manifestations tended to follow the appearance of cutaneous manifestations, but the frequency of minor muscular manifestations was high over the entire course of the disease.3)Laboratory findings showed increases in serum aldolase and serum creatine kinase with significant frequency when compared with adult patients (p<0.01 and p<0.05, respectively). Elevated serum aldolase most often occurred prior to or at the time of the appearance of muscular manifestations, suggesting its usefulness in early diagnosis. The positive rates for the antinuclear antibody on HEp‐2 cells and anti‐DNA antibody were significantly lower in children than in adults (p<0.001 and p<0.05, respectively).4)There were no cases of juvenile DMS complicated by malignant tumors, interstitial pneumonia, or pulmonary fibrosis. There were also no deaths, and the rate of “remission or improvement” was significantly higher than in adult DMS cases (p<0.05). Adult cases which remained the same or worsened usually presented with intractable muscular manifestations. In children, however, the cutaneous manifestations were more difficult to treat.The above conclusions from this statistical study suggest the possibility that juvenile DMS is an entity separate fro

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03264.x

年代:1992

数据来源: WILEY

摘要:

AbstractThis is the first report of a Japanese girl with Proteus syndrome. She presented with growth acceleration and precocious development of the left breast as well as macrodactyly, hemihypertrophy, a subcutaneous preaxillary mass, portwine stains, connective tissue nevi, and a depigmented macule. All these abnormalities were confined to the left side of her body. Although most of the manifestations fit those of Proteus syndrome, the presence of the portwine stains and hemihypertrophy also suggested Klippel‐Trenaunay‐Weber syndrome. The findings in our patient suggest that the most important characteristic distinguishing Proteus syndrome from Klippel‐Trenaunay‐Weber syndrome isthepresence of functional abnormalities such as a growth spurt and precocious breast development. Proteus syndrome may be genetically different from the Klippel‐Trenaunay‐Web

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03265.x

年代:1992

数据来源: WILEY

摘要:

AbstractA 34‐year‐old Japanese male had leg pain, edema of the legs, hypohidrosis, whorl‐like opacities of the bilateral cornea, bilateral subcapsular cataracts, and chest discomfort on exercise. He had no characteristic angiokeratomas but did have telangiectases. The electrocardiogram revealed high voltage. The echocardiogram revealed mild mitral regurgitation. The α‐galactosidase A activity in cultured lymphoblasts was deficient (0.5 nmol/h/mg protein). Electron microscopic examination of the skin revealed lamellar cytoplasmic inclusions in the endothelial cells, pericytes, and fibroblasts. He had a G—A transition at nucleotide 982 in the coding sequence of the α‐galactosidase A gene which resulted in a glycine to arginine amino acid substitution at residue 328. His uncle also had leg pain, edema of the legs, hypohidrosis, and chest pain on exercise. He had no characteristic angiokeratomas but did have telangiectases. Cardiovascular examination revealed hypertrophic cardiomyopathy and stenoses of coronary arteries. Electron microscopic examination of the skin revealed lamellar cytoplasmic inclusions in the endothelial cells, pericytes, an

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03266.x

年代:1992

数据来源: WILEY

摘要:

AbstractA 35‐year‐old woman exhibited bluish‐brown discoloration of her buccal mucosa suggesting malignant melanoma. Histopathological examination revealed that the pigment was not melanin but caused by metal deposits. Electron microscopically, metallic particles were located on the lamina densa of basal laminae at mucosal epithelium, nerve fibers, and bloodvessels and on the microfilaments of elastic fibers as well as in macrophages and fibroblasts. Electron microscopic point X‐ray microanalysis revealed that these metallic particles were composed of Ag, Se, Fe, Co, Cu, and S. Analysis suggests that these metals were derived from dental amalgam and that the discoloration was caused by amalgam

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03267.x

年代:1992

数据来源: WILEY

摘要:

AbstractTwo cases of eccrine hidrocystoma, one of which is a “classic” Robinson type and the other a Smith type, were reported. The walls of both cysts consisted mainly of two layers of flat or low cuboidal epithelial cells with eosinophilic cytoplasm. Immunostaining for S‐100 protein was negative in the cells of the cyst wall of the Robinson type and only weakly positive in the inner luminal layer of the Smith type. Electron microscopically, the Smith type showed double‐layered cuboidal lining and cell membrane interdigitations as junctions of neighboring cells without any characteristics of the secretory segment of sweat glands, indicating substantial similarity to those of the intradermal portion of the eccrine swe

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03268.x

年代:1992

数据来源: WILEY

摘要:

AbstractA 48‐year‐old Japanese female who had had chronic ATL for 4 years suddenly developed vesicles on the palms and soles. Histologically, these bullae were specific lesions of ATL. After a tendency to ward improvement, a crisis appeared with increases in the following: peripheral white blood cells, atypical lymphocytes, CD25 positive cells, serum LDH, and soluble IL‐2R. Palmoplantar bullae, a rather rare finding, may be indicative of a following c

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1992.tb03269.x

年代:1992

数据来源: WILEY