ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00989.x

年代:1976

数据来源: WILEY

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00990.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTIncorporation of some tritiated amino acids in normal and pathological human epidermis was studied by autoradiography. In the case of methionine, glycine and histidine, silver particles were detected in the cytoplasm of nucleated cells. In a test after 1–2 hours incubation the density of these particles was greater in the upper squamous cell layer as compared with the lower layer. In the case of tyrosine, phenylalanine, valine and leucine, the particles were distributed more densely in the lower squamous layer as compared with other layers. After 4–6 hours incubation, all of these amino acids were observed more uniformly in the entire epidermal layers except the horny layer, the labelling being highest in the basal layer.It may be interpreted here that the incorporation into the upper layers after 1–2 hours is related to the enzyme activity or energy of the epidermal cell, and that into the lower layers, especially the basal layer after 4–6 hours, to protein synthesis accompanied by cellular proliferation.In parakeratotic epidermis associated with psoriasis, chronic dermatitis, verruca vulgaris, etc., the radioactive particles of3H‐tyrosine, phenylalanine, valine and leucine were found in both the upper and lower squamous layers after 1–2 hours incubation, while they were observed only in the lower squamous layer in the normal epidermis. A similar tendency was found in epidermis with hyper‐ and parakeratosis induced by radiation with ultraviolet lights or stripping of the horny layer. The more the parakeratotic changes, the greater is the tendency.It is therefore suggested that protein synthesis or metabolism occuring in each level of the epidermis is accelerated by a shortened life‐span in the parakeratotic epidermis.In ichthyosis vulgaris, the so‐called keratogenous zone is distinctly seen as a narrow layer immediately beneath the horny layer, showing fairly intensive labelling from3H‐glycine.In the dyskeratotic cells of skin cancer and molluscum, a few silver particles were distributed wi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00981.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTThree cases of chromomycosis caused byFonsecaea pedrosoiwere treated by oral administration of 5‐fluorocytosine. Complete cure was achieved in 2 cases which had been symptomatically mild, while the third, with a long history, was not completely cured although it showed a transient improvement of clinical symptoms. No side effects were observed in all 3 cases during the treatment perio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00982.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTLeprosy has been regarded as a host dependent disease that presents an immunological spectrum. Various parameters ofin vivoandin vitrocell‐mediated and humoral responses in different forms of leprosy were studied. Lepromatous leprosy patients showed hyper gammaglobulinemia with significantly elevated levels of IgG and IgA while tuberculoid leprosy patients showed raised IgG levels.Cutaneous reactions to lepromin antigen were significantly depressed in patients with lepromatous leprosy while those to PPD were normal. Mitogenic response of peripheral leucocytes to phytoheamagglutinin was considerably depressed in untreated lepromatous leprosy and normal in tuberculoid leprosy. Dapsone‐treated patients with lepromatous leprosy showed a normal or enhanced response. Contributory factors were also found in plasma. T‐lymphocytes were significantly reduced in lepromatous leprosy with high bacillary load. B‐cells were increased in lepromatous patients. Tuberculoid leprosy patients had normal values.Phagocytic and bacteriolytic activity of blood derived macrophages was not altered in the lepromatous or tuberculoid p

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00983.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTA case of multicentric reticulohistiocytosis is presented, with typical clinical, roentgenologic, and histologic features. The characteristic giant cells were seen in skin nodules, mucosal nodules, synovial and bony lesions of the joints, and tenosynovial cysts. Corticosteroid therapy produced a subjective improvement, but the arthritis mutilans did not improve.Laboratory studies showed iron‐deficiency anemia, hypertriglyceridemia (hyperprebetalipo‐proteinemia), a low response to the unstimulated and stimulated nitroblue tetrazolium test of neutrophils, and a decreased activity in the glucose‐6‐phosphate dehydrogenase of polimorphonuclear leucocytes before corticosteroid therapy. The histochemical examination of the material in the giant cells showed a lipid (a phospholipid and a neutral fat), and a polysaccharide attached to a protein (a muco‐ or glycoprotein). Electron microscopic studies of the giant cells indicated that their cytoplasm contained numerous dense granules clustered in the vicinity of the Golgi apparatus. In some areas the granules appeared to be developing from the Golgi vesicles and cisternas.It seemed likely that the storage material was produced within the giant cells. We also discussed the pathogenesis of this

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00984.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTIn order to characterize the pemphigus antigen(s), the binding sites of phytohemagglutinins and pemphigus antibody were studied by the combined use of fluorescein‐labelled Concanavalin A (Con A‐FITC), Phytohemagglutinin‐P‐FITC (PHA‐P‐FITC) and pemphigus antibody. Reciprocal blocking test of PHA‐P and pemphigus antibody revealed that the binding sites of PHA‐P and pemphigus antibody were independent, whereas those of Con A and pemphigus antibody were very closely related though not identical. Pemphigus skin with deposition of IgG or with presumable saturation of the binding sites with high‐titred antibody showed capability to Con A binding. Treatment of normal skin sections with Hyaluronidase or Neuraminidase did not supply any clue to the histochemical analysis of pemphigus antigen(s), since the binding sites of pemphigus antibody were destroyed in the buffer, prior to the enzyme digestion. These results suggested that the binding sites of pemphigus antibody in the intercellular areas consist, at least, of carbohydrates or glycoproteins that contain alpha‐methyl‐D‐mannoside or a

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00985.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTA case of protothecosis affecting the skin of the face of a young Chinese girl due toPrototheca wickerhamiiis reported from Hong Kong. The disease persisted for eighteen years, was slowly progressive with local spread and extension to neighbouring conjunctival mucosa, and to the contralateral cheek by way of lymphatics, without systemic involvement.The source of infection could not be traced, and factors relating to the mode of onset were not known. There was no apparent defect in the immune mechanism in the otherwise healthy patient.Several antibiotics including polymyxin B, and amphotericin B given by various routes had failed to control the disease.The evolution of different patterns of protothecosis is discussed in the light of scanty case reports in the literature.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00986.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTSoluble epidermal glycoproteins reactive with Concanavalin A (Con A) were eluted by means of affinity chromatography using Con A‐conjugated Sepharose 4B. Studies on their immunogenicity and antigenicity revealed that the main components of pemphigus antigens are not identical with surface saccharides reactive with Con

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00987.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTThe effects of corticosteroid administration on the immune deposits in the skin of reversed passive Arthus reaction (RPAR) using horseradish peroxidase as antigen were studied. Immune deposits were seen on vascular walls, on collagen fibers, at the dermo‐epidermal junction, at perifollicular regions, on mononuclear cells and in PMN‐leukocytes. By corticosteroid administration the amount of these immune deposits was most obviously reduced within the first 30 minutes after the induction of the RPAR. At 1, 4, 8, 24 and 48 hours after induction of the reaction there were no significant differences in the amount and the extent of the immune deposits on the vascular walls, on the collagen fibers and on the mononuclear cells, between the sections treated by corticosteroid and those of non‐treated specimens. The infiltration of PMN‐leukocytes and their uptake of immune complexes were maximum at 4 hours after the injection and the number of these infiltrated PMN‐leukocytes and the amount of immune complexes which were taken by them were slightly reduced by corticosteroid admin

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb00988.x

年代:1976

数据来源: WILEY